Oct4, Sox2, Klf4, c-My (OSKM) gene therapy in the hypothalamus prolongs fertility and ovulation in female rats.

In middle-aged (MA) female rats, we have demonstrated that intrahypothalamic gene therapy for insulin-like growth factor-I (IGF-I) extends the regular cyclicity of the animals beyond 10 months (the age at which MA rats stop ovulating). Here, we implemented long-term OSKM gene therapy in the hypothalamus of young female rats. The main goal was to extend fertility in the treated animals.

Stress and telomere length in leukocytes: Investigating the role of GABRA6 gene polymorphism and cortisol.

Telomere length (TL) is considered a biomarker of aging, and short TL in leukocytes is related to age and stress-related health problems. Cumulative lifetime stress exposure has also been associated with shorter TL and age-related health problems, but the mechanisms are not well understood. We tested in 108 individuals whether shorter TL in leukocytes is observed in individuals with the GABRA6 TT genotype, which has been associated with dysregulation of hypothalamic-pituitary-adrenal axis activity (the main biological stress system) compared to the CC genotype.

Oral health-related quality of life in primary Sjögren's Syndrome.

Background: Primary Sjögren Syndrome (pSS) is an autoimmune disease that usually affects salivary glands. Research about the impact of oral health in quality of life of patients with pSS is scarce.

Objectives: to describe the characteristics of oral involvement in patients with pSS; To assess quality of life related to oral health (QOL-OH); to determine association between QOL-OH and saliva production, disease activity, and damage.

Point-of-care ultrasound in the diagnosis of hepatic gas gangrene.

Hepatic gas gangrene (HGG) is a rare but life-threatening condition typically caused by anaerobic bacteria such as Clostridium perfringens, though Gram-negative bacteria like Escherichia coli and Klebsiella species have also been implicated. Traditionally diagnosed via computed tomography (CT), point-of-care ultrasound (POCUS) has emerged as a valuable tool in critical care settings for its non-invasive, bedside utility. We report the case of a 51-year-old female with choledochal syndrome secondary to cholangiocarcinoma who developed HGG following left extended hepatectomy and biliary reconstruction.

Proteomic analysis of Trichoderma harzianum secretome and their role in the biosynthesis of zinc/iron oxide nanoparticles.

The fungal green synthesis of nanoparticles (NPs) has gained great interest since it is a cost-effective and easy handling method. The process is simple because fungi secrete metabolites and proteins capable of reducing metal salts in aqueous solution, however the mechanism remains largely unknown. The aim of this study was to analyze the secretome of a Trichoderma harzianum strain during the mycobiosynthesis process of zinc and iron nanoparticles.

Current level, sources, and risk of human exposure to PAHs, PBDEs and PCBs in South American outdoor air: A critical review.

This study provides comprehensive overview of the current level, sources and human exposure risk to hazardous polycyclic aromatic hydrocarbons (PAHs), polybrominated diphenyl ethers (PBDEs), and polychlorinated biphenyls (PCBs) in South American outdoor air. Research documents were obtainable for only 6 countries within the target period (2014 - 2024). For all contaminants, urban concentrations exceeded that of rural/remote locations.

Safety and efficacy of satralizumab in patients with generalised myasthenia gravis (LUMINESCE): a randomised, double-blind, multicentre, placebo-controlled phase 3 trial.

Background: Evidence from preclinical studies suggests that IL-6 signalling has the potential to modulate immunopathogenic mechanisms upstream of autoantibody effector mechanisms in patients with generalised myasthenia gravis. We aimed to assess the safety and efficacy of satralizumab, a humanised monoclonal antibody targeting the IL-6 receptor, in patients with generalised myasthenia gravis.

Methods: LUMINESCE was a randomised, double-blind, placebo-controlled, multicentre, phase 3 study at 105 sites, including hospitals and clinics, globally.

Radiofrequency evoked potentials: A new window into the nociceptive system.

Objective: To describe the cortical evoked potentials in response to radiofrequency stimulation (RFEPs) in human volunteers.

Methods: Seventeen healthy volunteers participated in an experimental session in which radiofrequency (RF) and electrical (ES) stimulation were applied to the dorsum of the hands and feet. EEG was recorded to evaluate evoked responses for each stimulus modality and stimulation site.

Specific Rosetta-based protein-peptide prediction protocol allows the design of novel cholinesterase inhibitor peptides.

The search for novel cholinesterase inhibitors is essential for advancing treatments for neurodegenerative disorders such as Alzheimer's disease (AD). In this study, we employed the Rosetta pepspec module, originally developed for designing peptides targeting protein-protein interactions, to design de novo peptides targeting the peripheral aromatic site (PAS) of acetylcholinesterase (AChE) and butyrylcholinesterase (BChE). A total of nine peptides were designed for human AChE (hAChE), T.

Exposure to environmentally relevant levels of DEHP during development modifies the distribution and expression patterns of androgen receptors in the anterior pituitary in a sex-specific manner.

DEHP is a prevalent phthalate with wide industrial applications and well-documented endocrine-disrupting effects, including the potential disruption of AR signaling in different tissues. The present study aimed to investigate the effects of gestational and lactational exposure to environmentally relevant DEHP concentrations on AR expression and subcellular localization in the pituitary gland, the master endocrine organ, with a focus on gonadotroph cells by in vivo and in vitro approaches. After DEHP exposure during gestation and lactation, a sex-specific modulation was detected in AR-positive pituitary cells and AR protein expression as assessed through flow cytometry and western blot.

Optimal Bilio-Pancreatic Limb (BPL) Length in One Anastomosis Gastric Bypass (OAGB) Surgery.

Background: One Anastomosis Gastric Bypass (OAGB) is a modification of Mason's loop bypass procedure, which has become a well-established procedure in the field of Bariatric and Metabolic surgery (BMS). However, the optimal length of Biliopancreatic Limb (BPL) in OAGB remains an ongoing debate.

Objective: This review aims to analyse the current trends and evidence regarding different BPL lengths in OAGB and their impact on outcomes.

Current State of Research on Health-Promoting Functional Properties in Berry-Based Foods.

Purpose Of Review: This review aims to consolidate recent findings on the development and functional validation of berry-based foods while proposing guidelines for future advancements.

Recent Findings: Current investigations on berry-based functional foods (dairy and bakery products, snacks, etc.) emphasize their potential health benefits, including antioxidant effects, glycemic control, enzyme modulation, among others.

Evaluation of the evidence-based practices for the management of PCOS in the Latin America context: the consensus of the Latin American Association of Gynecological Endocrinology (ALEG).

Objectives: Polycystic Ovary Syndrome (PCOS) is a complex condition affecting approximately 1 in 10 women of reproductive age. However, limited data are available regarding the specific characteristics and needs of women with PCOS in Latin America. This consensus sought to evaluate the evidence-based practices for the management of PCOS for Latin American populations, consolidate regional insights, identify eventual gaps in implementation and identify key research opportunities.

RNA Virus Discovery Sheds Light on the Virome of a Major Vineyard Pest, the European Grapevine Moth ().

The European grapevine moth () poses a significant threat to vineyards worldwide, causing extensive economic losses. While its ecological interactions and control strategies have been well studied, its associated viral diversity remains unexplored. Here, we employ high-throughput sequencing data mining to comprehensively characterize the virome, revealing novel and diverse RNA viruses.

Neuroprotective Effect of Melatonin Loaded in Human Serum Albumin Nanoparticles Applied Subconjunctivally in a Retinal Degeneration Animal Model.

Background/objectives: Neurodegenerative ocular diseases, such as age-related macular degeneration (AMD) and glaucoma, represent growing public health concerns. Oxidative stress plays a key role in their development, damaging retinal cells and accelerating disease progression. Melatonin (Mel) is a potent antioxidant with neuroprotective properties; however, it faces limitations such as low solubility.

3D-Printed Tablets of Nifurtimox: In Vitro and In Vivo Anti- Studies.

: Chagas disease is a neglected tropical disease caused by infection with the parasite . Benznidazole and nifurtimox are the only approved drugs for treating this condition, but their low aqueous solubility may lead to erratic bioavailability. This work aimed for the first time to formulate tablets of nifurtimox by hot melt extrusion coupled with 3D printing as a strategy to increase drug dissolution and the production of tablets with dosage on demand.

Development and Characterization of Trihexyphenidyl Orodispersible Minitablets: A Challenge to Fill the Therapeutic Gap in Neuropediatrics.

Trihexyphenidyl (THP) has been widely used for over three decades as pediatric pharmacotherapy in patients affected by segmental and generalized dystonia. In order to achieve effective and safe pharmacotherapy for this population, new formulations are needed. The aim of this work is the development of trihexyphenidyl orodispersible minitablets (ODMTs) for pediatric use.

Alkaloid Profile, Anticholinesterase and Antioxidant Activities, and Sexual Propagation in (Amaryllidaceae).

, a recently described endemic species from southern Peru, belongs to the Amaryllidaceae family and is known for its diversity of alkaloids. Amaryllidoideae have been studied for their diverse biological activities, particularly for their properties in treating neurodegenerative diseases. This work examines the alkaloidal profile using GC-MS and UPLC-MS/MS of alkaloid-enriched extracts obtained from the leaves and bulbs of and their inhibitory activity against acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) enzymes.

Herbicidal Formulations with Plant-Based Compounds to Control , and Weeds.

Numerous studies have shown the potential effect of bioactive agents against weeds. In this study, we developed two binary formulations with nonanoic acid, citral, or thymoquinone as herbicides and evaluated their physicochemical properties. The presence of the bioactive compounds in the formulations was confirmed through FTIR spectroscopy.

Brid., a Bryophyta from Northwest Argentina as a Source of Antioxidants and Antimicrobials.

In recent years, numerous studies have emerged on the biological activities of bryophytes and their potential for therapeutic use. However, mosses appear to be a relatively overlooked group. The objective of this study was to conduct a phytochemical analysis of one hydroalcoholic extract of and to evaluate its potential as an antioxidant and antimicrobial agent.

Jurassic Osmundaceous Landscapes in Patagonia: Exploring the Concept of Ecological Stasis in the Deseado Massif, Argentina.

Herein, we report the presence of a plant paleocommunity, dominated by ferns of the family Osmundaceae, structurally preserved from the only known Mesozoic, fossiliferous geothermal deposits, from the La Matilde Formation (Middle-Upper Jurassic) in the Deseado Massif of Southern Patagonia, Argentina. A total of 13 siliceous chert blocks sampled in an area of approximately 250 m, preserving a monotypic assemblage dominated by Osmundaceae embedded within its original swampy substrate, are documented. Additional Osmundaceae and fewer ferns and conifers are present in the stratigraphically continuous, adjacent chert levels.

Cellulose-Based Materials and Their Application in Lithium-Sulfur Batteries.

Lithium-sulfur (Li-S) batteries are promising candidates for next-generation energy storage due to their high energy density, cost-effectiveness, and environmental friendliness. However, their commercialization is hindered by challenges, such as the polysulfide shuttle effect, lithium dendrite growth, and low electrical conductivity of sulfur cathodes. Cellulose, a natural, renewable, and versatile biopolymer, has emerged as a multifunctional material to address these issues.

2,4-Dichlorophenoxyacetic Acid in the Gas and Crystal Phases and Its Intercalation in Montmorillonite-An Experimental and Theoretical Study.

Many properties of 2,4-dichlorophenoxyacetic acid (2,4-D) depend on its molecular environment, such as whether it is an isolated molecule, a dimer, or in a crystalline state. The molecular geometry, conformational analysis, and vibrational spectrum of 2,4-D were theoretically calculated using Density Functional Theory (DFT) methods. A new slightly more stable conformer was found, which is different to those previously reported.