474 results match your criteria: "Aquaculture Center[Affiliation]"

The microbiota of teleost fish has gained a great deal of research attention within the past decade, with experiments suggesting that both host-genetics and environment are strong ecological forces shaping the bacterial assemblages of fish microbiomes. Despite representing great commercial and scientific importance, the catfish within the family , specifically the blue and channel catfish, have received very little research attention directed toward their gut-associated microbiota using 16S rRNA gene sequencing. Within this study we utilize multiple genetically distinct strains of blue and channel catfish, verified via microsatellite genotyping, to further quantify the role of host-genetics in shaping the bacterial communities in the fish gut, while maintaining environmental and husbandry parameters constant.

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Clinostomum spp. (Digenea: Clinostomidae) are a group of trematodes commonly found in the buccal cavity and oesophagus of a variety of piscivorous birds. The metacercariae, colloquially known as "yellow grubs," have been reported from a diverse group of freshwater fishes worldwide.

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Efficacy of Benzocaine, Eugenol, and Menthol as Anesthetics for Freshwater Angelfish.

J Aquat Anim Health

September 2018

Campus Fernandópolis, Brazil University, Estrada Projetada F-1, s/n Fazenda Santa Rita, 15600-000, Fernandópolis, São Paulo, Brazil.

For the production and commercialization of ornamental fish species, it is indispensable to collect biometric data that facilitate the selection of animals for trade and genetic improvement of the stock. However, during the handling process, fish receive more stress if proper anesthetics are not used. Thus, application of appropriate anesthetics is an important tool for minimizing stress in animals.

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Teleostei present great plasticity regarding sex change. During sex reversal, the whole gonad including the germinal epithelium undergoes significant changes, remodeling, and neoformation. However, there is no information on the changes that occur within the interstitial compartment.

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Cytochemical features of the digestive tract mucosa of Hemisorubim platyrhynchos (Siluriformes: Pimelodidae).

J Fish Biol

June 2018

São Paulo State University - UNESP, Department of Biological Sciences, School of Sciences, 14-01 Engenheiro Luiz Edmundo Carrijo Coube Avenue, 17033-360, Bauru, SP, Brazil.

Membranous organelles, acid glycoconjugates and lipids were characterized in the digestive tract mucosa of Hemisorubim platyrhynchos by cytochemistry techniques. Two types of mucous-secreting cells were observed in the digestive tract epithelium: goblet cells in the oesophagus and intestine and epithelial cells in the stomach. These cells had a Golgi apparatus more developed than the other cell types.

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In this paper, we have described for the first time a semiquantitative method to evaluate histopathological damage, taking the degree of Neoechinorhynchus buttnerae attachment to the intestinal wall of the tambaqui (Colossoma macropomum), an important species in Brazilian aquaculture, into account. Twelve specimens of tambaqui were collected from a fish farm. Their bowels were removed and divided into seven morphologically distinct portions according to density and distribution of the parasite studies.

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Columnaris disease, caused by the Gram-negative bacterium , is one of the most prevalent fish diseases worldwide. An exceptionally high level of genetic diversity among isolates of has long been recognized, whereby six established genomovars have been described to date. However, little has been done to quantify or characterize this diversity further in a systematic fashion.

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SummaryThe aim of this study was to describe the effect of temperature on the fertilization, early developmental stages, and survival rate of two Neotropical catfishes Pimelodus maculatus and Pseudopimelodus mangurus. After fertilization, the eggs were incubated at 22°C, 26°C, and 30°C, which resulted in fertilization rates of 96.95 ± 1.

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The pirapitinga, (Characiformes, Serrasalmidae), is a fish from the Amazon basin and is considered to be one of the main native species used in aquaculture production in South America. The objectives of this study were: (1) to perform liver transcriptome sequencing of pirapitinga through NGS and then validate a set of microsatellite markers for this species; and (2) to use polymorphic microsatellites for analysis of genetic variability in farmed stocks. The transcriptome sequencing was carried out through the Roche/454 technology, which resulted in 3,696 non-redundant contigs.

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Tambaqui, , is the main native fish species produced in Brazil, and is an important species for genetic improvement in aquaculture. In addition, breeding studies on this species can be optimized with the use of molecular markers associated with productive phenotypes. The objective of the present study was to test the performance of growth traits and resistance to the bacteria, , in association with microsatellite markers in .

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Phosphorus (P) is an essential mineral for the development and maintenance of the vertebrate skeletal system. Modulation of P levels is believed to influence metabolism and the physiological responses of gene expression. In this study, we investigated the influence of dietary P on skeletal deformities and osteocalcin gene expression in zebrafish (), and sought to determine appropriate levels in a diet.

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In fish, many factors can affect reproduction during in vitro fertilization, therefore determination of the factors that affect affecting gamete quality is needed. However, few studies have focused on gamete quality and the ploidy status. This study was conducted to elucidate whether oocyte storage can affect ploidy status, survival, and embryo viability in the characid species Astyanax altiparanae.

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In fish with external fertilization, sperm must reach the oocyte through the micropyle to enter the cytoplasm. Fertilization success is then influenced by characteristics of oocytes or sperm. In this study, we evaluated oocyte morphology and sperm motility parameters and their effects on the inseminating dose in a teleost fish Astyanax altiparanae.

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Characterization of two channel catfish, Ictalurus punctatus, glucocorticoid receptors and expression following an acute stressor.

Comp Biochem Physiol A Mol Integr Physiol

February 2018

USDA/ARS Warmwater Aquaculture Research Unit, Thad Cochran National Warmwater Aquaculture Center, P.O. Box 38, Stoneville, MS 38776, USA.

Two channel catfish glucocorticoid receptor genes, ipGR1 (NR3C1_1) and ipGR2 (NR3C1_2) were partially characterized. Identification and analysis of the genomic organization of two channel catfish glucocorticoid (GC) receptors (GRs) revealed differences in the lengths of exons 1 and 2 and the addition of an extra 27-bp exon inserted after exon 2 in the GR1 gene, yielding a 9-aa insert in the receptor protein. Sequence of the 9-aa insert in ipGR1 (WRARQNTHG) is unique compared to other teleost fish GRs.

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The influence of midazolam on heart rate arises from cardiac autonomic tones alterations in Burmese pythons, Python molurus.

Auton Neurosci

December 2017

Aquaculture Center (CAUNESP), São Paulo State University (UNESP), Via Prof. Paulo Donato Castellane, n/n, Jaboticabal, SP 14884-900, Brazil; Department of Zoology and Botany, São Paulo State University (UNESP), Cristóvão Colombo Street, 2265, São José do Rio Preto, SP 15054-000, Brazil; National Institute of Science and Technology in Comparative Physiology (INCT - FAPESP/CNPq), Via 24A, 1515, Rio Claro, SP 13506-900, Brazil. Electronic address:

The GABA receptor agonist midazolam is a compound widely used as a tranquilizer and sedative in mammals and reptiles. It is already known that this benzodiazepine produces small to intermediate heart rate (HR) alterations in mammals, however, its influence on reptiles' HR remains unexplored. Thus, the present study sought to verify the effects of midazolam on HR and cardiac modulation in the snake Python molurus.

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The production of triploid yellowtail tetra is a key factor to obtain permanently sterile individuals by chromosome set manipulation. Flow cytometric analysis is the main tool for confirmation of the resultant triploids individuals, but very few protocols are specific for species. The current study has developed a protocol to estimate DNA content in this species.

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spp. are responsible for significant losses in important wild and cultured fish species worldwide. Recent phylogenomic investigations have determined that bacteria historically classified as actually represent three genetically distinct yet phenotypically ambiguous taxa with various degrees of pathogenicity in different hosts.

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Bayesian analysis improves experimental studies about temporal patterning of aggression in fish.

Behav Processes

December 2017

Universidade Estadual Paulista Júlio Mesquita Filho (UNESP/IBILCE), Zoology and Botany Department, R. Cristóvão Colombo, 2265, CEP 15054-000, São José do Rio Preto, SP, Brazil; Aquaculture Center of Sao Paulo State University (CAUNESP), Brazil. Electronic address:

This study aims to describe a Bayesian Hierarchical Linear Model (HLM) approach for longitudinal designs in fish's experimental aggressive behavior studies as an alternative to classical methods In particular, we discuss the advantages of Bayesian analysis in dealing with combined variables, non-statistically significant results and required sample size using an experiment of angelfish (Pterophyllum scalare) species as case study. Groups of 3 individuals were subjected to daily observations recorded for 10min during 5days. The frequencies of attacks, displays and the total attacks (attacks+displays) of each record were modeled using Monte Carlo Markov chains.

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Ultrastructure of spermatozoa of spider crabs, family Mithracidae (Crustacea, Decapoda, Brachyura): Integrative analyses based on morphological and molecular data.

J Morphol

December 2017

Departamento de Biologia Aplicada, Laboratório de Morfologia do Invertebrados (IML), Unesp Aquaculture Center (Caunesp) e IEAMar, Universidade Estadual Paulista (UNESP), Prof. Donato Castellane, S/N, Jaboticabal, SP, 14884-900, Brazil.

Recent studies based on morphological and molecular data provide a new perspective concerning taxonomic aspects of the brachyuran family Mithracidae. These studies proposed a series of nominal changes and indicated that the family is actually represented by a different number and representatives of genera than previously thought. Here, we provide a comparative description of the ultrastructure of spermatozoa and spermatophores of some species of Mithracidae in a phylogenetic context.

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This study aimed to examine the gonadal morphology of diploid and triploid fish through stereological analysis. Triploid individuals were obtained after temperature shock (40°C for 2 min) at 2 min post-fertilization and reared until 175 days post-fertilization (dpf). Intact eggs were used to obtain the diploids.

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Different β-glucans improve the growth performance and bacterial resistance in Nile tilapia.

Fish Shellfish Immunol

November 2017

São Paulo State University, Aquaculture Center of Unesp, Jaboticabal, Via de Acesso Prof. Paulo Donato Castelane, 14.884-900, Jaboticabal, São Paulo, Brazil.

The role of glucan as a biologically active immunomodulator has been well documented for more than 40 years. However, the wide diversity of β-glucan forms and the extraction process has implications for the benefits of these compounds. Biorigin developed two samples of β-glucans using different biotechnological processes.

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is a Gram-negative bacillus that has recently been implicated in disease outbreaks in tilapia and zebrafish. We report here the complete and annotated genome sequence of an isolate from a Nile tilapia (), which contains a chromosome of 3,630,639 bp and two plasmids.

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