3,923 results match your criteria: "Animal Research Center[Affiliation]"

With a multitude of HCC mouse models available, choosing the one that most closely resembles human HCC can be challenging. This study addresses this gap by conducting a comprehensive transcriptomic similarity analysis of widely used HCC mouse models. In this study, RNA-seq was performed on a model induced by double knockout of P53 and Pten via CRISPR/Cas9 in HBV-transgenic mice.

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Malnutritional stress during gestation is a well-established driver of metabolic disfunction in offspring. Extended exposure to malnutrition requires metabolic plasticity as the animal shifts toward a catabolic state. In this paper we demonstrate the influence of malnutrition throughout gestation on uterine artery hemodynamics and the metabolism of the dam and neonate.

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One-carbon metabolism (OCM) is a series of connected pathways involving the methionine-folate cycles, transsulfuration, polyamine synthesis, nucleotide synthesis, free-radical scavenging, and energy metabolism. These pathways functionally depend upon amino acids (methionine, glycine, and serine), vitamins (folate, B, B, and B), and minerals (sulfur, cobalt, and zinc). Growing bodies of research indicate that in beef cattle, physiological stage, nutritional plane, diet, species (Bos taurus vs.

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Combined Treatment Methods for Removal of Antibiotics from Beef Wastewater.

ACS Omega

December 2024

U.S. Meat Animal Research Center, Agricultural Research Service, United States Department of Agriculture, Clay Center, Nebraska 68933, United States.

Use of antibiotics is common practice in agriculture; however, they can be released into the environment, potentially causing antimicrobial resistance. Naturally mined diatomaceous earth with bentonite was tested as a remediation material for tylosin, chlortetracycline, and ceftiofur in wastewater from a beef cattle feedlot. Langmuir binding affinity in 10 mM sodium phosphate buffer at pH 6.

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The second form of gonadotropin-releasing hormone (GnRH-II) and its receptor (GnRHR-II) are abundantly produced within the porcine testis and immunolocalize within the seminiferous tubules, suggesting a role in spermatogenesis and/or sperm function. The objective of this study was to quantify GnRH-II and GnRHR-II abundance within boar reproductive tract tissues and examine their role in porcine sperm function. Immunoblotting revealed GnRHR-II abundance was 12-fold greater (P < 0.

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Nanoplatform for synergistic therapy constructed via the co-assembly of a reduction-responsive cholesterol-based block copolymer and a photothermal amphiphile.

Mater Today Bio

December 2024

Department of Polymer Materials, School of Materials Science and Engineering, Shanghai University, Nanchen Street 333, Shanghai, 200444, China.

The goal of combination cancer therapy, including chemo-phototherapy, is to achieve highly efficient antitumor effects while minimizing the adverse reactions associated with conventional chemotherapy. Nevertheless, enhancing the contribution of non-chemotherapeutic strategies in combination therapy is often challenging because this requires multiple active ingredients to be encapsulated in a single delivery system. However, most commonly used photothermal reagents are challenging to be loaded in large quantities and have poor biocompatibility.

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Mpox is a zoonotic disease caused by the Monkeypox virus (MPXV), and since May 2022, tens of thousands of cases have been reported in non-endemic countries. We aimed to evaluate the suitability of different sample types for mpox diagnostic and assess the temporal dynamics of viral load. We evaluated 1914 samples from 953 laboratory-confirmed cases.

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Microbiome and metabolomics reveal the effect of gut microbiota on liver regeneration of fatty liver disease.

EBioMedicine

December 2024

Division of Hepatobiliary and Pancreatic Surgery, Department of Surgery, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310003, China; NHC Key Laboratory of Combined Multi-Organ Transplantation, Hangzhou, 310003, China; Department of Hepatobiliary and Pancreatic Surgery, Shulan (Hangzhou) Hospital, Zhejiang Shuren University School of Medicine, Hangzhou, 310015, China; Jinan Microecological Biomedicine Shandong Laboratory, Jinan, 250117, China. Electronic address:

Background: Metabolic dysfunction-associated fatty liver disease (MAFLD) is associated with impaired regenerative capacity and poor postoperative prognosis following hepatectomy. Previous research has highlighted the importance of the gut-liver axis in the physiological and pathological processes of the liver. However, the contribution of gut bacteria to the regeneration of livers with MAFLD and its metabolic regulatory mechanisms remain elusive.

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Effect of curcumin regulated memory Th7 cells in mice with DSS-induced colitis.

Int Immunopharmacol

January 2025

School of Nursing, Jiangxi University of Chinese Medicine, Nanchang 330004, Jiangxi Province, China; Formula-Pattern Research Center of Jiangxi University of Chinese Medicine, Nanchang 330004, Jiangxi Province, China. Electronic address:

Abnormal activation or dysfunction of memory helper T (mTh) cells is closely associated with the development of ulcerative colitis (UC). Curcumin (Cur), the main component of turmeric, plays a critical role in the treatment of UC due to its favorable anti-inflammatory and immunomodulatory bioactivities. However, whether Cur modulates mTh7 cells to alleviate UC is unknown.

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Context Antral follicle count (AFC) is reflective of ovarian reserve and linked to reproductive performance in mammalian females. We previously demonstrated earlier upregulation of endometrial oxytocin receptor transcription in heifers with low AFC, indicating an earlier onset of luteolysis. Aims We aimed to support the earlier onset of luteolysis in non-pregnant heifers with a low number of antral ovarian follicles (Open Low AFC) and hypothesized a reduced abundance of luteinizing hormone/choriogonadotropin receptor (LH/CG-R ) and increased abundance of thrombospondin-1 (THBS1 ) in luteal tissue of those heifers.

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Ultraflexible neural electrodes have shown superior stability compared with rigid electrodes in long-term recordings, owing to their low mechanical mismatch with brain tissue. It is desirable to detect neurotransmitters as well as electrophysiological signals for months in brain science. This work proposes a stable electronic interface that can simultaneously detect neural electrical activity and dopamine concentration deep in the brain.

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Article Synopsis
  • Enterohemorrhagic Escherichia coli (EHEC) strains, linked to red meat outbreaks, pose serious global food safety issues, especially since current detection methods often yield false positives due to non-pathogenic strains.
  • This study focused on developing and validating four new hydrolysis probe assays specifically designed to detect pathogenic E. coli serogroups O26, O103, O111, and O121, showing an impressive accuracy of 94.8% in identifying virulent strains.
  • The newly standardized assays offer reliable detection tools for food laboratories worldwide, helping to mitigate waste and financial losses from incorrect positive test results associated with harmless non-pathogenic E. coli.
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SLC25A1 regulates placental development to ensure embryonic heart morphogenesis.

Development

November 2024

MOE Key Laboratory of Model Animal for Disease Study, Model Animal Research Center State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University Medical School, Nanjing 210093, China.

22q11.2 deletion syndrome (22q11.2DS) is the most common chromosomal microdeletion syndrome.

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The length of ewe productive life (LPL), defined as the number of days between the first and last lambing, is a key indicator of ewe longevity and is directly related to the sustainability of the sheep industry. Therefore, the primary objective of this study was to investigate systematic effects influencing LPL in Katahdin sheep. The LPL of 10,474 Katahdin ewes (69.

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Testosterone deficiency aggravates diet-induced non-alcoholic fatty liver disease by inducing hepatocyte ferroptosis via targeting BMAL1 in mice.

Int Immunopharmacol

January 2025

School of Pharmacy, Zhejiang Chinese Medical University, Hangzhou 310053, China; Laboratory Animal Research Center, Academy of Chinese Medical Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, China; Zhejiang Key Laboratory of Blood-Stasis-Toxin Syndrome, Zhejiang Chinese Medical University, Hangzhou 310053, China. Electronic address:

Background: Testosterone deficiency is linked to an increased prevalence of non-alcoholic fatty liver disease (NAFLD), although the mechanisms underlying this association are not fully understood. Ferroptosis, a regulated cell death pathway driven by iron-dependent lipid peroxidation, has been suggested to play a role in NAFLD pathogenesis. Since testosterone deficiency is associated with lipid disorders and iron deposition, we hepothesize that ferroptosis may be involved in the pathogenesis of diet-induced NAFLD exacerbated by testosterone deficiency.

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Objective: To evaluate the therapeutic effects and explore the mechanisms behind caloric restriction achieved through time-restricted feeding (CR) in inhibiting mouse tumors, providing a theoretical basis and data support for future CR diet-assisted anticancer treatment protocols.

Methods: C57BL/6 and BALB/c mice were divided into four cell line groups. Each group was further split into normal diet (ND) and a CR diet groups.

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CRISPR Diagnostics for Quantification and Rapid Diagnosis of Myotonic Dystrophy Type 1 Repeat Expansion Disorders.

ACS Synth Biol

December 2024

Division of Animal Genetics, Laboratory Animal Research Center, Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan.

Repeat expansion disorders, exemplified by myotonic dystrophy type 1 (DM1), present challenges in diagnostic quantification because of the variability and complexity of repeat lengths. Traditional diagnostic methods, including PCR and Southern blotting, exhibit limitations in sensitivity and specificity, necessitating the development of innovative approaches for precise and rapid diagnosis. Here, we introduce a CRISPR-based diagnostic method, REPLICA (peat-rimed ocating of nherited disease by s3), for the quantification and rapid diagnosis of DM1.

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Bovine viral diarrhea virus (BVDV) infection during pregnancy is a significant contributor to reproductive failures in cattle. The bovine receptor for BVDV (CD46) was previously edited with a six amino acid substitution (G82QVLAL to A82LPTFS) and shown to have significantly reduced BVDV susceptibility in a Gir heifer calf. Since a role for CD46 has been proposed in mammalian fertilization, our objective was to assess the edited heifer's fertilization rates, early embryonic development, and germline transmission conformation of the edit.

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TSG101 depletion dysregulates mitochondria and PML NBs, triggering MAD2-overexpressing interphase cell death (MOID) through AIFM1-PML-DAXX pathway.

Cell Death Dis

November 2024

National Resource Center for Mutant Mice, MOE Key Laboratory of Model Animals for Disease Study, Model Animal Research Center, Medical School of Nanjing University, Nanjing, 210061, China.

Article Synopsis
  • * The process of MOID is influenced by the release of mitochondrial AIFM1 mediated by proteins PML and DAXX, indicating a complex interplay between mitochondrial function and cell survival mechanisms.
  • * Both MAD2 and TSG101 interact at PML nuclear bodies during interphase, and specific phosphorylation states of TSG101 are essential for this localization, emphasizing a vital regulatory pathway in controlling cell death and survival in cancer contexts.
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Direct reprogramming of fibroblasts into spiral ganglion neurons by defined transcription factors.

Cell Prolif

November 2024

MOE Key Laboratory of Model Animal for Disease Study, Department of Otolaryngology Head and Neck Surgery, Jiangsu Provincial Key Medical Discipline (Laboratory), The Affiliated Drum Tower Hospital of Medical School and the Model Animal Research Center of Medical School, Nanjing University, Nanjing, China.

Degeneration of the cochlear spiral ganglion neurons (SGNs) is one of the major causes of sensorineural hearing loss and significantly impacts the outcomes of cochlear implantation. Functional regeneration of SGNs holds great promise for treating sensorineural hearing loss. In this study, we systematically screened 33 transcriptional regulators implicated in neuronal and SGN fate.

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Deficiency of myeloid NPC1 exacerbates liver injury and fibrosis by impairing macrophage efferocytosis.

J Adv Res

November 2024

Laboratory Animal Research Center, School of Medicine, Chongqing University, Chongqing 400044, China. Electronic address:

Article Synopsis
  • Niemann-Pick C1 (NPC1) is a cholesterol transport protein linked to liver issues such as hepatomegaly and acute liver failure, but its exact role in these conditions is not understood.
  • The study investigates the role of NPC1 in liver injury, focusing on how its presence in macrophages affects liver health and inflammation.
  • Findings reveal that increased NPC1 protects against liver damage by enhancing macrophage cleanup of dead cells, while a lack of NPC1 worsens inflammation and tissue damage in liver injury models.*
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The fat mass and obesity associated (FTO) gene, previously identified as a pivotal genetic locus associated with adiposity, has recently been linked to various cancers. In this study, we established an FTO knockout (KO) cell line in porcine iliac artery endothelial cells (PIECs) utilizing CRISPR/Cas9 technology to systematically investigate the gene's function and effect through transcriptomic and metabolomic analysis. Our results revealed significant gene expression and metabolic profiles differences between the FTO KO and wild-type (WT) cells.

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