Characterization of the Normal Portal and Hepatic Blood Flow of Adult Holstein-Friesian Cows.

In the past, hepatic blood flow in cows was invasively characterized to investigate different pathologies and physiological conditions. However, hepatic blood flow can be easily evaluated with transabdominal Doppler ultrasound. Sixteen healthy adult non-lactating, non-pregnant Holstein-Friesian cows were examined using B-mode and Doppler ultrasound between the right flank and 9 intercostal space to establish the best approach to the different parts of the portal and hepatic vein systems, and determine normal blood flow characteristics.

Variation in trace element content between liver lobes in cattle. How important is the sampling site?

The aim of the present study was to establish the pattern of lobular distribution of trace elements in the liver of cattle. The objective was to determine which part of the liver would provide accurate estimation of the trace element content of the whole organ. Liver samples were obtained from 10 Holstein-Friesian (HF), 10 Galician Blond (GB) and 10 GBxHF crosses (all aged 10 months) at slaughter.

Validation of a simple sample preparation method for multielement analysis of bovine serum.

Here we propose a single acid digestion (SAD) sample preparation method for ICP-MS analysis of animal serum samples to determine trace element contents. The method was evaluated in comparison with a commonly used procedure involving dilution of samples in an alkaline solution (AKD). In the SAD procedure, aliquots (1 mL) of bovine serum samples were treated at low temperature with a mixture of concentrated nitric acid and hydrogen peroxide.

Morphological and immunohistochemical study of the rabbit vomeronasal organ.

The characterization of the rabbit mammary pheromone, which is sensed by the main olfactory system, has made this species a unique model for the study of pheromonal communication in mammals. This discovery has brought attention to the global understanding of chemosensory communication in this species. Chemocommunication is mediated by two distinct organs located in the nasal cavity, the main olfactory epithelium and the vomeronasal organ (VNO).

Importance of breed aptitude (beef or dairy) in determining trace element concentrations in bovine muscles.

The aim of this study was to determine the concentrations of various trace elements in muscles with different oxidative/glycolytic profiles (cardiac [CA]; diaphragm [DI], as oxidative; trapezius [TR], as intermediate oxidative/glycolytic; and semimembranosus [SM], as glycolytic muscle) of ten dairy-aptitude (Holstein-Friesian, HF), ten beef-aptitude (Galician Blonde, GB) and ten cross-breed (GBxHF) calves. The type of muscle was a highly significant factor in relation to the concentrations of all elements, whereas breed was only significant for Fe, Mn and Zn in the SM muscle. The concentrations of the main trace elements (Cu, Fe, Se and Zn) were significantly lower in GB and GBxHF than in HF, that were mainly associated with differences in the oxidative/glycolytic profile, probably due to the muscular hypertrophy characteristic of heavily muscled breeds.

Trace Element Concentrations in Beef Cattle Related to the Breed Aptitude.

Animal feed has traditionally been supplemented with trace elements at dietary concentrations well above physiological needs. However, environmental concerns have led to calls for better adjustment of mineral supplementation to actual physiological needs and, in this context, consideration of breed-related differences in trace element requirements. The aim of this study was to analyze trace element concentrations in the main breeds used for intensive beef production in northern Spain (Holstein-Friesian [HF], Galician Blonde [GB], and GB × HF cross).

Subcellular distribution of hepatic copper in beef cattle receiving high copper supplementation.

Previous studies of intensively reared cattle in NW Spain have reported significantly higher copper (Cu) accumulation in the liver in Holstein-Friesian (HF) animals than in Galician Blonde (GB) or GBxHF crosses when receiving a diet supplemented at the maximum Cu concentrations allowed in the EU legislation (35mg/kg). The present study aimed to evaluate whether this difference is due to the pattern of subcellular accumulation of Cu in the liver. For this purpose, liver samples from 10 GB, 9 HF and 10 GBxHF young bulls were analysed to determine the content of metallothionein (MT) and Cu and zinc (Zn) (in the liver (Cu-liver and Zn-liver) and bound to metallothionein (Cu-MT and Zn-MT)).

Saturated salt solution: a further step to a formaldehyde-free embalming method for veterinary gross anatomy.

In the field of veterinary anatomy, most of the specimens used in practical sessions are perfused with fixatives. Thus, they can be used for a longer time reducing the number of animals for educational purposes. Formalin is the most commonly used fixative, consisting of a 37% formaldehyde solution.

Hepatic concentrations of copper and other metals in dogs with and without chronic hepatitis.

Objectives: Defects in copper metabolism have been described in several dog breeds, and recently, it has been suggested that changes in other essential trace elements could be involved in the pathogenesis of hepatic disease. This study measured hepatic copper accumulation and its interactions with other essential trace and toxic metals in dogs diagnosed with chronic hepatitis.

Methods: Liver samples of 20 chronic hepatitis and 20 healthy dogs were collected.