Backbone resonance assignments of PhoCl, a photocleavable protein.

PhoCl is a photocleavable protein engineered from a green-to-red photoconvertible fluorescent protein by circular permutation, and has been used in various optogenetic applications including precise control of protein localization and activity in cells. Upon violet light illumination, PhoCl undergoes a β-elimination reaction to be cleaved at the chromophore, resulting in spontaneous dissociation into a large empty barrel and a small C-terminal peptide. However, the structural determinants and the mechanism of the PhoCl photocleavage remain elusive, hindering the further development of more robust photocleavable optogenetic tools.

PRDX2 induces tumor immune evasion by modulating the HDAC3-Galectin-9 axis in lung adenocarcinoma cells.

Background: PRDX2 is significantly expressed in various cancers and is associated with the proliferation of tumor cells. Nonetheless, the precise mechanism of PRDX2 in tumor immunity remains incompletely understood. This study aims to investigate the impact of PRDX2, which is highly expressed in lung adenocarcinoma, on T cells in the tumor immune microenvironment, and its immune action target to promote the immune escape of lung cancer cells, to provide a theoretical basis for lung adenocarcinoma treatment with PRDX2 as the target.

Balanced dung beetle optimization algorithm based on parameter substitution and escape strategy.

Dung Beetle algorithm is an intelligent optimization algorithm with advantages in exploitation ability. However, due to the high randomness of parameters, premature convergence and other reasons, there is an imbalance between exploration and exploitation ability, and it is easy to fall into the problem of local optimal solution. The purpose of this study is to improve the optimization performance of dung beetle algorithm and explore its engineering application value.

Optimal dose and type of exercise improve walking velocity in adults with Parkinson's disease: a systematic review and Bayesian network meta-analysis.

To examine the dose-response relationship between specific types of exercise for improving walking velocity in Parkinson's disease (PD). This systematic review and network meta-analysis included searches of PubMed, Medline, Embase, PsycINFO, Cochrane Library, and Web of Science were searched from inception until February 18th, 2024. Data analysis was performed using R software with the MBNMA and RJAGS packages.

Serum autoantibody-based biomarkers for prognosis in early-stage lung cancer patients with surgical resection.

Background: Lung cancer is the cancer with the highest morbidity and mortality in the world. With the increasing diagnosis rate of patients with early-stage lung cancer, surgery treatment becomes an option for more patients. However, there is a lack of effective indicators to assess the risk of recurrence after lung cancer surgery.

Crepidamycins A-E, pyranonaphthoquinones from endophytic Streptomyces sp. MG-F-1 of Dendrobium crepidatum by the co-culture strategy.

On the basis of the co-culture strategy, five previously undescribed S-bridged pyranonaphthoquinones, crepidamycins A-E (1-5) and five known analogues (6-10) were isolated from a medicinal plant endophytic Streptomyces sp. MG-F-1 in Dendrobium crepidatum with Bacillus cereus MG-1. The structures and absolute configurations of 1-5 were elucidated by the interpretation of data from detailed spectroscopic analysis and electronic circular dichroism spectra, together with consideration of the biogenetic origins.

Bonding Features of Period 3 and Period 1 Two-Dimensional Superatoms.

Despite being studied for almost two centuries, aromaticity has always been a controversial concept. We previously proposed a unified aromatic rule for π-conjugated systems by two-dimensional (2D) superatomic-molecule theory, where benzenoid rings are treated as period 2 2D superatoms (3π-N, 4π-O, 5π-F, 6π-Ne) and, further, bond to form 2D superatomic molecules. Herein, to build a 2D periodic table, we further extend the theory to period 3 (7π-P, 8π-S, 9π-Cl, 10π-Ar) and period 1 (1π-H, 2π-He) elements.

Aucubin Promotes BMSCs Proliferation and Differentiation of Postmenopausal Osteoporosis Patients by Regulating Ferroptosis and BMP2 Signalling.

Postmenopausal osteoporosis (PMOP) is a chronic systemic bone metabolism disorder. Promotion in the patterns of human bone marrow mesenchymal stem cells (hBMSCs) differentiation towards osteoblasts contributes to alleviating osteoporosis. Aucubin, a natural compound isolated from the well-known herbal medicine Eucommia, was previously shown to possess various pharmacological effects.

Defects Calculation and Accelerated Interfacial Charge Transfer in a Photoactive MOF-Based Heterojunction.

Photocatalytic hydrogen production is currently considered a clean and sustainable route to meet the energy and environmental issues. Among, heterojunction photocatalysts have been developed to improve their photocatalytic efficiency. Defect engineering of heterojunction photocatalysts is attractive due to it can perform as electron trap and change the band structure to optimize the interfacial separation rate of photogenerated electron-hole pairs.

Functional Polyolefins and Composites.

Polyolefins are simple hydrocarbons that require additional chemical modifications or functional additives to give them custom functions. Recent research in the development of functional polyolefins has surpassed the traditional approach of simply improving surface properties by incorporating polar moieties. Creating custom functionalized polyolefins by using specific functional units has attracted increasing attention.

HCTTI: High-Performance Heterogeneous Computing Toolkit for Tissue Image Stain Normalization.

Whole slide imaging (WSI) has transformed diagnostic medicine, particularly in the field of cancer diagnosis and treatment. The use of deep learning algorithms for predicting WSIs has opened up new avenues for advanced medical diagnostics. Additionally, stain normalization can reduce the color and intensity variations present in WSI from different hospitals.

Comprehensive Review and Meta-Analysis of Psychological and Pharmacological Treatment for Intermittent Explosive Disorder: Insights From Both Case Studies and Randomized Controlled Trials.

Intermittent explosive disorder (IED) is characterized by sudden, disproportionate outbursts of anger that can severely impact individuals' quality of life, causing difficulties in maintaining relationships, issues at work or school and potential legal troubles. This study aimed to systematically review and meta-analyse the effectiveness of psychological and pharmacological treatments for IED, drawing insights from both case studies and randomized controlled trials (RCTs). A total of 12 RCTs and 14 case studies were included in this comprehensive analysis.

Cardiovascular protective effects of natural flavonoids on intestinal barrier injury.

Natural flavonoids may be utilized as an important therapy for cardiovascular diseases (CVDs) caused by intestinal barrier damage. More research is being conducted on the protective properties of natural flavonoids against intestinal barrier injury, although the underlying processes remain unknown. Thus, the purpose of this article is to present current research on natural flavonoids to reduce the incidence of CVDs by protecting intestinal barrier injury, with a particular emphasis on intestinal epithelial barrier integrity (inhibiting oxidative stress, regulating inflammatory cytokine expression, and increasing tight junction protein expression).

Genetic analyses identify brain functional networks associated with the risk of Parkinson's disease and drug-induced parkinsonism.

Brain functional networks are associated with parkinsonism in observational studies. However, the causal effects between brain functional networks and parkinsonism remain unclear. We aimed to assess the potential bidirectional causal associations between 191 brain resting-state functional magnetic resonance imaging (rsfMRI) phenotypes and parkinsonism including Parkinson's disease (PD) and drug-induced parkinsonism (DIP).

Time-resolved spectroscopy uncovers deprotonation-induced reconstruction in oxygen-evolution NiFe-based (oxy)hydroxides.

Transition-metal layered double hydroxides are widely utilized as electrocatalysts for the oxygen evolution reaction (OER), undergoing dynamic transformation into active oxyhydroxides during electrochemical operation. Nonetheless, our understanding of the non-equilibrium structural changes that occur during this process remains limited. In this study, utilizing in situ energy-dispersive X-ray absorption spectroscopy and machine learning analysis, we reveal the occurrence of deprotonation and elucidate the role of incorporated iron in facilitating the transition from nickel-iron layered double hydroxide (NiFe LDH) into its active oxyhydroxide.

Mechanism of - for treatment of primary liver cancer: analysis with network pharmacology, molecular docking and validation.

Objectives: To investigate the active ingredients in Hedyotis diffusa-Scutellaria barbata D. Don and the main biological processes and signaling pathways mediating their inhibitory effect on primary hepatocellular carcinoma (HCC).

Methods: The core intersecting genes of HCC and the two drugs were screened from TCMSP, Uniport, Genecards, and String databases using Cytoscape software, and GO and KEGG enrichment analyses of the intersecting genes were conducted.

Inhibiting miR-155-5p promotes proliferation of human submandibular gland epithelial cells in primary Sjogren's syndrome by negatively regulating the PI3K/AKT signaling pathway PIK3R1.

Objectives: To investigate the mechanism mediating the regulatory effect of miR-155-5p on proliferation of human submandibular gland epithelial cells (HSGECs) in primary Sjogren's syndrome (pSS).

Methods: Dual luciferase reporter assay was used to verify the targeting relationship between miR-155-5p and the PI3K/AKT pathway. In a HSGEC model of pSS induced by simulation with TRAIL and INF-γ, the effects of miR-155-inhibitor-NC or miR-155 inhibitor on cell viability, cell cycle, apoptosis and proliferation were evaluated using CKK8 assay, flow cytometry and colony formation assay.

scRNA-seq reveals involvement of monocytes in immune response in SLE patients.

Background: Systemic Lupus Erythematosus (SLE) is a typical autoimmune disease characterized by a complex pathogenesis and a strong genetic predisposition. The study of inflammatory response in SLE monocytes is not very clear, and exploring the inflammatory factors of monocytes is beneficial to discover new diagnostic targets.

Results: Using scRNA-seq technology, we obtained the quantitative changes in circulating immune cells and various cellular immune metabolic profiles between SLE patients and healthy volunteers.

Ubiquitination in diabetes and its complications: A perspective from bibliometrics.

Background: Diabetes has a substantial impact on public health, highlighting the need for novel treatments. Ubiquitination, an intracellular protein modification process, is emerging as a promising strategy for regulating pathological mechanisms. We hypothesize that ubiquitination plays a critical role in the development and progression of diabetes and its complications, and that understanding these mechanisms can lead to new therapeutic approaches.

Descriptions of four species of Polyxenida Verhoeff, 1934 (Diplopoda, Penicillata) from China, including one new species and one new record.

This study describes a new species of Polyxenida from China, , along with a species newly recorded from China: (Miyosi, 1947), and provides additional descriptions of Ishii & Liang, 1990 and Ishii & Liang, 1990. The study conducted mitochondrial cytochrome oxidase subunit I (COI) sequencing for all four species and constructed a phylogenetic tree based on the molecular data. The comprehensive morphological descriptions and molecular analyses confirm the addition of one new species and one newly recorded species for the Polyxenida fauna of China, elevating the total number of known Polyxenida species in the country from 10 to 12.

Hypoxia-responsive nanoparticles for fluorescence diagnosis and therapy of cancer.

Hypoxia, caused by rapid tumor growth and insufficient oxygen supply, is a defining characteristic of numerous solid tumors and exerts a significant influence on tumor growth, metastasis, and invasion. Early diagnosis and effective killing of tumor cells are crucial for cancer treatment. In recent years, the emergence of nanomaterials has overcome the difficulties in the delivery of chemotherapeutic drugs and contrast agents to tumor area.

Efficient CH oxidation to C1/C2 oxygenates over cluster-dispersing Rh decorated ZSM-5.

Crafting highly dispersed active metal sites on catalysts is an optimal method for improving the catalytic reactivity and stability, as it would improve atomic utilization efficiency, enhance reactant adsorption and activation ability through unique geometric and electronic properties. In this study, two synthesis methods were employed (ammonia evaporation (AE) and the impregnation method (IM)) to load Rh species onto the ZSM-5 support in order to attain tunable dispersivity, during which a 1.25-fold increase in the total yield of liquid oxygenated products (32 433.

Bearing characteristics and damage rules of regenerated rock mass.

This study investigates the bearing characteristics and damage evolution of regenerative rock masses formed under varying geological conditions through uniaxial loading tests, numerical simulations, and theoretical derivations. Regenerative rock mass samples with different water-cement ratios and cementing materials were prepared, and the mechanical behavior during the loading process was analyzed. The results indicate that the secondary damage process can be divided into three stages: pre-peak, weakening, and friction.

Tetrahedral AlO Cage: A Superchalcogen Atom.

Superatoms are stable clusters that mimic the chemical behavior of individual atoms in the periodic table. Many endeavors have been devoted to the design and characterization of various superatoms, while engineering superatoms to mimic the chemistry of chalcogens remains a challenge. In this paper, we present a new superchalcogen by evaluating a hollow tetrahedral AlO cluster with theoretical calculations.