Lung-Selective Delivery of mRNA-Encoding Anti-MERS-CoV Nanobody Exhibits Neutralizing Activity Both In Vitro and In Vivo.

: The Middle East Respiratory Syndrome Coronavirus (MERS-CoV) is a highly pathogenic virus causing severe respiratory illness, with limited treatment options that are mostly supportive. The success of mRNA technology in COVID-19 vaccines has opened avenues for antibody development against MERS-CoV. mRNA-based antibodies, expressed in vivo, offer rapid adaptability to viral mutations while minimizing long-term side effects.

Material Visual Perception and Discharging Robot Control for Baijiu Fermented Grains in Underground Tank.

Addressing the issue of excessive manual intervention in discharging fermented grains from underground tanks in traditional brewing technology, this paper proposes an intelligent grains-out strategy based on a multi-degree-of-freedom hybrid robot. The robot's structure and control system are introduced, along with analyses of kinematics solutions for its parallel components and end-effector speeds. According to its structural characteristics and working conditions, a visual-perception-based motion control method of discharging fermented grains is determined.

A Spatiotemporal Feature-Based Approach for the Detection of Unlicensed Taxis in Urban Areas.

Unlicensed taxis seriously disrupt the transportation market order, and threaten passenger safety. Therefore, this paper proposes a method for identifying unlicensed taxis based on travel characteristics. First, the vehicle mileage and operation time are calculated using traffic surveillance bayonet data, and variance analysis is applied to identification indicators for unlicensed taxis.

Style Transfer of Chinese Wuhu Iron Paintings Using Hierarchical Visual Transformer.

Within the domain of traditional art, Chinese Wuhu Iron Painting distinguishes itself through its distinctive craftsmanship, aesthetic expressiveness, and choice of materials, presenting a formidable challenge in the arena of stylistic transformation. This paper introduces an innovative Hierarchical Visual Transformer (HVT) framework aimed at achieving effectiveness and precision in the style transfer of Wuhu Iron Paintings. The study begins with an in-depth analysis of the artistic style of Wuhu Iron Paintings, extracting key stylistic elements that meet technical requirements for style conversion.

Prediction of SPT-07A Pharmacokinetics in Rats, Dogs, and Humans Using a Physiologically-Based Pharmacokinetic Model and In Vitro Data.

SPT-07A, a D-borneol, is currently being developed in China for the treatment of ischemic stroke. We aimed to create a whole-body physiologically-based pharmacokinetic (PBPK) model to predict the pharmacokinetics of SPT-07A in rats, dogs, and humans. The in vitro metabolism of SPT-07A was studied using hepatic, renal, and intestinal microsomes.

Light-Powered Self-Translation of an Asymmetric Friction Slider Using a Liquid Crystal Elastomer String Oscillator.

In recent years, there have been many studies focused on improving the performance of active materials; however, applying these materials to active machines still presents significant challenges. In this study, we introduce a light-powered self-translation system for an asymmetric friction slider using a liquid crystal elastomer (LCE) string oscillator. The self-translation system was composed of a hollow slide, two LCE fibers, and a mass ball.

Density Functional Theory Insight in Photocatalytic Degradation of Dichlorvos Using Covalent Triazine Frameworks Modified by Various Oxygen-Containing Acid Groups.

Dichlorvos (2,2-dichlorovinyl dimethyl phosphate, DDVP) is a highly toxic organophosphorus insecticide, and its persistence in air, water, and soil poses potential threats to human health and ecosystems. Covalent triazine frameworks (CTFs), with their sufficient visible-light harvesting capacity, ameliorated charge separation, and exceptional redox ability, have emerged as promising candidates for the photocatalytic degradation of DDVP. Nevertheless, pure CTFs lack effective oxidative active sites, resulting in elevated reaction energy barriers during the photodegradation of DDVP.

Application of Phosphate-Based Binders for the Stabilization and Solidification of Metal-Contaminated Soil: Mechanisms and Efficacy Evaluation.

At present, contamination due to toxic metals is a global concern. The management of problems caused by heavy metals relies on stabilization/solidification, which is the most effective technique for the control of metal pollution in soil. This study examined the immobilization efficiency of various phosphate-based binders (NaPO, NaHPO, NaHPO), in addition to ordinary Portland cement (OPC), MgO, and CaO, for the stabilization of multi-metal-contaminated soils.

Advances in Soil Amendments for Remediation of Heavy Metal-Contaminated Soils: Mechanisms, Impact, and Future Prospects.

Heavy metal contamination is a critical factor contributing to soil degradation and poses significant environmental threats with profound implications for ecosystems and human health. Soil amendments have become an effective strategy to address these challenges by reducing heavy metal hazards and remediating contaminated soils. This review offers a comprehensive analysis of recent advancements in soil amendments for heavy metal-contaminated soils, with a focus on natural, synthetic, natural-synthetic copolymer, and biological amendments.

Regulation of Isoleucine on Colonic Barrier Function in Rotavirus-Infected Weanling Piglets and Analysis of Gut Microbiota and Metabolomics.

Rotavirus (RV) is a significant contributor to diarrhea in both young children and animals, especially in piglets, resulting in considerable economic impacts on the global pig industry. Isoleucine (Ile), a branched-chain amino acid, is crucial for regulating nutrient metabolism and has been found to help mitigate diarrhea. This study aimed to assess the impact of isoleucine supplementation in feed on colonic barrier function, colonic microbiota, and metabolism in RV-infected weanling piglets.

(Curtis) P. Karst. Immunomodulatory Protein Has the Potential to Improve the Prognosis of Breast Cancer Through the Regulation of Key Prognosis-Related Genes.

: Breast cancer in women is the most commonly diagnosed and most malignant tumor. Although luminal A breast cancer (LumA) has a relatively better prognosis, it still has a persistent pattern of recurrence. (Curtis) P.

Identification of a Potential PGK1 Inhibitor with the Suppression of Breast Cancer Cells Using Virtual Screening and Molecular Docking.

Background/objectives: Breast cancer is the second most common malignancy worldwide and poses a significant threat to women's health. However, the prognostic biomarkers and therapeutic targets of breast cancer are unclear. A prognostic model can help in identifying biomarkers and targets for breast cancer.

An Improved Algorithm to Extract Moiré Fringe Phase for Wafer-Mask Alignment in Nanoimprint Lithography.

This paper proposes an improved algorithm based on the phase extraction of the Moiré fringe for wafer-mask alignment in nanoimprint lithography. The algorithm combines the strengths of the two-dimensional fast Fourier transform (2D-FFT) and two-dimensional window Fourier filtering (2D-WFF) to quickly and accurately extract the fundamental frequencies of interest, eliminate noise in the fundamental frequency band by using the threshold of the local spectrum, and effectively suppress spectral leakage by using a Gaussian window with outstanding sidelobe characteristics while overcoming their limitations, such as avoiding the time-consuming parameter adjustment. The phase extraction accuracy determines the misalignment measurement accuracy, and the alignment accuracy is enhanced to the nanometer level, which is 15.

Assembly and Valence Modulation of Ordered Bimetallic MOFs for Highly Efficient Electrocatalytic Water Oxidation.

Metal synergy can enhance the catalytic performance, and a prefabricated solid precursor can guide the ordered embedding, of secondary metal source ions for the rapid synthesis of bimetallic organic frameworks (MM'-MOFs) with a stoichiometric ratio of 1:1. In this paper, containing well-defined binding sites was synthesized by mechanical ball milling, which was used as a template for the induced introduction of Fe ions to successfully assemble the ordered bimetallic (where denotes template-directed synthesis of MOF-74). Its electrocatalytic performance is superior to that of the conventional one-step-synthesized (where denotes one-step synthesis of MOF-74), and the ratio of the two metal sources, Co and Fe, is close to 1:1.

New Species and Records of Townes, 1965 (Hymenoptera, Ichneumonidae, Campopleginae), from China.

Campopleginae, the third largest subfamily of Ichneumonidae, has been one of the most investigated ichneumonids from the perspective of biological control. Despite their importance as potential biological control agents, species of campoplegine are among the most poorly studied insect groups. In this study, one new species of the genus Townes, 1965, sp.

Optimizing Yeast Homologous Recombination for Splicing Large Coronavirus Genome Fragments.

Reverse genetics is a useful tool for studying viruses and developing vaccines for coronaviruses. However, constructing and manipulating the coronavirus genome in can be time-consuming and challenging due to its large size and instability. Homologous recombination, a genetic manipulation mechanism found in organisms, is essential for DNA repair, gene recombination, and genetic engineering.

Plasma-Activated Medium Inhibited the Proliferation and Migration of Non-Small Cell Lung Cancer A549 Cells in 3D Culture.

Lung cancer is the most common type of malignant tumor worldwide. Plasma-activated medium (PAM) is an innovative cancer treatment method that has received considerable scientific attention. The objective of this study is to evaluate the effects of PAM on the anti-tumor characteristics of non-small cell lung cancer (NSCLC) cells in two-dimensional (2D) and three-dimensional (3D) cultures.

ZFP36L2 Is a Potential Prognostic Marker of IL1β Osteosarcoma.

Osteosarcoma stands as the predominant bone malignancy afflicting children and young adults. Despite strides in treatment, the enduring reality is that the long-term survival rates for patients grappling with recurrences and metastases linger at a mere 30%. This underscores the pressing demand for novel prognostic markers and therapeutic avenues to improve outcomes and offer hope to those battling this formidable disease.

Research on the Effect Mechanism of Career-Specific Parental Support Promoting Meaning in Life of Chinese Higher Vocational College Students.

Adolescent students' meaning in life is an important topic of research in positive psychology and educational psychology. Meaning in life is of outstanding value to the whole career development of Chinese higher vocational college students (CHVCS), and parental behaviors have a profound impact on CHVCS' meaning in life. In order to explore the mechanism of the role of career-specific parental support in promoting CHVCS' meaning in life from the perspective of career, this study was based on career construction theory (CCT) and investigated 2498 CHVCS.

Multi-Element Fingerprinting Combined with Chemometrics for Identification of Seaweeds and Innovative Risk-Benefit Assessment.

Seaweeds are one of the major marine foods with high values. The diversity of seaweed species significantly impacts their quality and is closely linked to their purity and safety. For the first time, this study established a model to discriminate seaweed species using a multi-element fingerprinting approach for species identification.

Mass Spectrometry-Based Metabolomics Investigation on Two Different Seaweeds Under Arsenic Exposure.

Arsenic is a common toxic heavy metal contaminant that is widely present in the ocean, and seaweeds have a strong ability to concentrate arsenic, posing a potential risk to human health. This study first analyzed the arsenic content in two different seaweeds and then used an innovative method to categorize the seaweeds into low-arsenic and high-arsenic groups based on their arsenic exposure levels. Finally, a non-targeted metabolomic analysis based on mass spectrometry was conducted on seaweed from different arsenic exposure groups.

Developing a Simple and Feasible Process for the Crude Extraction of Livetins and Phosvitin from Egg Yolk.

Due to imbalanced demand favoring egg whites, the egg industry faces a surplus of egg yolk, limiting overall growth. This study designed a feasible process for the crude extraction of livetins and phosvitin (PV) and revealed the related separation mechanisms. Our method utilized a 1:9 egg yolk dilution at pH 6.

Joint User Association, Power Allocation and Beamforming for NOMA-Based Integrated Satellite-Terrestrial Networks.

This paper investigated a non-orthogonal multiple access (NOMA)-based integrated satellite-terrestrial network (ISTN), where each user can select to access a terrestrial base station (BS) or the satellite according to the capacity of BS and their individual transmission requirements. A two-stage algorithm is proposed to solve the achievable sum rate maximizing resource optimization problem. In the first stage, user associations are determined based on individual preference lists and the backhaul capacities of the access points (APs).

Characteristic Muscle Quality Parameters of Male Largemouth Bass () Distinguished from Female and Physiological Variations Revealed by Transcriptome Profiling.

Male largemouth bass () are often overlooked because females grow faster. We explored the value of male largemouth bass by comparing muscle nutrition, texture, and transcriptomes between males and females. Females grew faster than males ( < 0.

Effects of on Growth Performance, Carcass Quality, Serum Indicators, Intestinal Morphology, and Digestive Enzymes in Broilers.

The purpose of this experiment is to investigate how different doses of powder affect the growth performance, carcass quality, serum biochemical indexes, serum antioxidant and immunological index, intestinal morphology, and digestive enzyme activity of broilers. Four hundred chicks of a similar weight (1 day old) are randomly assigned to four groups of five replicates of 20 chicks each (half males and half females). The control group is fed a basal ration, and the experimental groups T1, T2, and T3 are supplemented with 6.