Measurement uncertainty for <711> Dissolution tests using Monte Carlo methods - Uncertainties arising from sampling and analytical steps.

Background: The dissolution test is a critical quality control method in the pharmaceutical industry, primarily used to assess drug bioavailability and ensure the consistency of manufactured batches. This test simulates the release of the active ingredient in the body and verifies compliance with specifications through multiple stages (e.g.

Sensitive and quick electrochemiluminescence biosensor for the detection of reactive oxygen species in seminal plasma based on the valence regulation of gold nanoclusters.

Background: Gold nanoclusters (AuNCs) obtained by electroreduction have excellent electrochemiluminescence (ECL) properties, and its ECL intensity is regulated by the valence state. In addition, their ECL signals can be rapidly quenched by reactive oxygen species (ROS). Based on this observation, a sensitive ROS biosensor was designed based on valence regulation of AuNCs.

Enhanced photocurrents for photoelectrochemical immunoassay of alpha-fetoprotein with Pt-functionalized BiOS nanoflowers.

Background: Designing heterojunctions with efficient electron-hole separation holds great promise for improving photoelectric response.

Results: Herein, we reported a multifunctional Pt co-catalyst-modified BiOS nanoflowers (BOS NFs) photocatalytic component for achieving an efficient photoelectric chemistry (PEC) immunosensor for alpha-fetoprotein (AFP). Briefly, the Pt co-catalyst improved the intrinsic band gap structure of BOS on the one hand, and on the other hand, it was able to achieve a rapid decomposition of hydrogen peroxide to hydroxyl radicals, which led to the improvement of electrochemical half-responses during the amplification of target immunosignals.

Self-validating photoelectrochemical/photoelectrochromic visual sensing platform for ciprofloxacin precise detection in milk.

Background: In the process of food production, ciprofloxacin (CIP), a highly prescribed fluoroquinolone antibiotic, is often excessively used to reduce the risk of bacterial infection. However, this overuse can cause severe harm to human health, including allergic responses, gastrointestinal complications, and potential renal dysfunction. The development of a robust and precise detection method for CIP is crucial, given the interconnection between food security and human health.

A nanopore-based label-free CRISPR/Cas12a system for portable and ultrasensitive detection of zearalenone.

Background: Food safety has become a serious global concern. Therefore, there is a need for effective detection technologies in this field. Currently, the development of effective on-site detection techniques is extremely important for food safety.

A ratiometric fluorescent biosensing platform based on CDs and AuNCs@CGO for patulin detection.

Background: Patulin (PAT) is a mycotoxin, usually found in fruit and their products, that can potentially be harmful to human health. In order to achieve rapid detection of mycotoxins and ensure the safety of food. This study reported a novel ratiometric fluorescent aptasensor for PAT detection.

A heterotypic tumor-on-a-chip platform for user-friendly combinatorial chemotherapeutic testing.

Background: Three-dimensional (3D) tumor microdevices are promising platform for biomimetic antitumor prediction and high-throughput chemotherapeutic screening and play crucial roles in the exploration of cancer-associated pharmaceutics and therapeutics. Traditional cell manipulation tools (e.g.

A liquid crystal-decorated aptasensing gadget for rapid monitoring of A549 cells: Future portable test kit for lung cancer diagnosis.

Background: Presented here is a straightforward detection system designed to track non-small cell lung cancer (specifically A549 cells) using a combination of liquid crystals (LCs) and aptamer sequences, marking a pioneering approach in this field. A change in the alignment of LCs from perpendicular to random status by the aptamer-cell complex altered the murky polarized background of the aptasensor to multicolored.

Results: The LC-designed aptasensor could determine A549 cancerous cells in the range of 2.

Lab-on-a-chip paper-based electrochemically assisted solid-phase microextraction and ion selective sensor for determination of naproxen in biological fluid samples.

Background: The integration of paper-based analytical devices with lab-on-a-chip technology has opened up new possibilities for miniaturized, rapid, low cost and portable diagnostic testing in resource limited settings. This work introduces an integrated lab-on-a-chip platform coupling paper-based analytical devices (LOC-PADs) for dual usage: electrochemically controlled solid-phase microextraction (EC-SPME) and determination of naproxen (NAP) by potentiometric ion-selective electrode (ISE) in biological samples.

Results: Conductive papers were successfully fabricated by a chemical procedure.

An ingenious chemiluminescence sensing strategy for recalcitrant triphenyl phosphate based on oxidant-free UV-activated MIL-100(Fe) gel system.

Background: Organophosphate flame retardants (OPFRs) are notorious emerging contaminants threatening the environment and human health. Triphenyl phosphate (TPHP), which has an extremely serious biotoxicity, is a typical harmful OPFR. Due to its wide use, TPHP has been discovered in various environmental mediums.

A novel and facile oxygen-activated time-temperature indicator with wide temperature monitoring range and good stability based on the laccase-like nanozyme.

Background: Time-Temperature Indicator (TTI) is an indicator device for real-time monitoring of the thermal history of the product. Due to the enzymatic reactions are affected by both time and temperature, enzymatic TTIs have been extensively studied and developed in recent years. However, enzymatic TTIs contain biologically active molecules (enzymes), which require high storage and use conditions.

A selective dual-signal electrochemical paper-based device using imprinted sensors for voltammetric and impedance analysis of 4-NQO and carcinoembryonic antigen (CEA).

Background: Carcinoembryonic Antigen (CEA) and 4-nitroquinoline-N-oxide (4-NQO) are cancer markers that play a crucial role in tumor risk assessment and early cancer diagnosis. Therefore, it is in demand to develop a fast, accurate, simple, and cost-effective method to detect these cancer markers for quick and early stage-cancer diagnosis and treatment.

Results: Herein, we report a dual signaling approach for direct and indirect signal transduction of cancer biomarker binding on molecularly imprinted-electrodes integrated on an ePAD, enabling sensitive and selective quantitative analysis of 4-NQO and CEA.

Deep learning assisted femtosecond laser-ablation spark-induced breakdown spectroscopy employed for rapid and accurate identification of bismuth brass.

Background: Owing to its excellent machinability and less toxicity, bismuth brass has been widely used in manufacturing various industrial products. Thus, it is of significance to perform rapid and accurate identification of bismuth brass to reveal the alloying properties. However, the analytical lines of various elements in bismuth brass alloy products based on conventional laser-induced breakdown spectroscopy (LIBS) are usually weak.

Optimization of nanoparticle-enhanced laser-induced breakdown spectroscopy for the hyperspectral chemical mapping of solid samples.

Background: Nanoparticle-enhanced laser-induced breakdown spectroscopy (NE-LIBS) uses the plasmonic effect of metallic nanoparticles deposited on solid sample surfaces to achieve a significant signal enhancement by lowering the breakdown threshold and elevating plasma temperature. NE-LIBS has been used for localized analysis, but NE-LIBS mapping of solids has rarely been done, due to several challenges. In this study, we scrutinized the performance of NE-LIBS hyperspectral mapping of solid samples, when the controlled deposition of nanoparticles was done using magnetron sputtering.

3D printed microplasma optical emission spectrometry coupled with ZIF-8 based dispersive solid-phase extraction for field analysis of waterborne arsenic.

Background: Arsenic contamination of drinking water has become a public health challenge over the world, particularly in Bangladesh, India, and China. Compared to the most used field test kits of waterborne arsenic, miniature microplasma atomic spectrometry retains advantages of accuracy, elemental specificity, and less matrix interference. Despite increased interest in arsenic detection by using miniature microplasma spectrometry, the improvements of its analytical performance, manufacturing cost and consistency still remain significant challenges.

Deep eutectic supramolecular polymers based HPLC stationary phase: Green synthesis strategy and promising application prospects.

Background: Deep eutectic solvents (DESs) have been widely and significantly applied in various fields due to their outstanding features such as low cost, easy preparation and good biodegradability. As novel derivatives of DESs, deep eutectic supramolecular polymers (DESPs) combine the macroscopic state of DESs with the covalent interactions of supramolecular polymers, which also possess the properties of DESs as multifunctional materials. Therefore, DESPs are believed to be promising candidates for separation science.

Simultaneous measurement of labile U(VI) concentration and (U/U) activity ratio using a Monophos®-based Diffusive Gradients in thin-films sampler.

Background: In a context of environmental monitoring around installations related to the nuclear fuel cycle, the Diffusive Gradient in Thin-films (DGT) technique captures the integrated concentration of U isotopes in their native environment, yielding comprehensive data on U origin (anthropogenic vs natural), total concentration, and mobility. However, for common deployment times (4-5 days) in moderately basic waters, none of the commercially available binding gels is adapted to measure the total U concentration. So, the development of novel DGT binding gels is timely.

A novel NIR fluorescent probe to image HNO during ferroptosis.

Background: As an important reactive nitrogen species (RNS), HNO has been identified as an essential signaling molecule in many physiological processes. Ferroptosis produces a large amount of reactive oxygen species and reactive nitrogen species. However, the detailed mechanism of HNO during process of ferroptosis is rarely reported, especially in the near-infrared range.

Oriental covalent immobilization of N-glycan binding protein via N-terminal selective modification.

Lectin affinity chromatography is one of powerful tools for the study of protein glycosylation. Different lectin proteins can recognize different structures of monosaccharides or oligosaccharide units, allowing for the selective separation of glycopeptides or glycoproteins containing different polysaccharide structures. However, the N-glycans were only partially captured by most of common lectins, reducing the coverage rate of identifying N-glycoconjugates.

Liquid-liquid phase separation-related signature predicts prognosis and therapeutic response in esophageal adenocarcinoma.

Background: Esophageal adenocarcinoma is a leading cause of mortality worldwide. New evidence indicates that liquid-liquid phase separation is related to malignancies. The current study aims at exploring the functions of liquid-liquid phase separation within esophageal adenocarcinoma.

Evaluation of analytical uncertainty in quantitative determination of elements - The case of boron.

Background: The uncertainty including accuracy and precision is the most vital factor that determines the overall quality of quantitative analysis. The uncertainty has been, however, evaluated relatively within the same analytical technique. Given this background, the present study evaluates the uncertainty on quantitative elemental analysis with a quasi-absolute approach.

Dual-emission rare-earth fluorescent nanomaterials for ratiometric and visual detection of N-acetylneuraminic acid and applications in information encryption and anti-counterfeiting.

N-acetylneuraminic acid (NANA) can be used as a biomarker for many types of cancers. Currently, there are various methods for detecting NANA but showing some shortcomings that limit the real-time diagnosis of cancer. In contrast, fluorescence analysis has obvious advantages such as low cost, fast response time, and easy operation, and it also enables visual detection for real-time cancer monitoring.