Nanoparticle-mediated light-driven LAMP combined with test strips for sensitive and rapid visual detection of antibiotic resistance genes.

Antibiotic resistance genes (ARGs) are markers of drug-resistant pathogens, monitoring them contributes to prevent resistance to drugs. The detection methods for ARGs including PCR and isothermal amplification are sensitive and selective. However, it may take several hours or cannot be used on spot.

Nuclear receptor 4A1 facilitates complete Freund's adjuvant-induced inflammatory pain in rats by promoting ferroptosis in spinal glial cells.

Glial cell-induced neuroinflammation in the spinal cord is the critical pathology underlying complete Freund's adjuvant (CFA)-induced inflammatory pain. Previously, we showed that spinal glial cells undergo ferroptosis after CFA injection, which may contribute to the development of neuroinflammation and inflammatory pain. However, the mechanism underlying the occurrence of ferroptosis during inflammatory pain remains unclear.

Effects of Different Non-Ionic Polysaccharides on the Heat-Induced Gelling Properties of Curdlan.

Curdlan's application is constrained by high gelation concentration, poor water solubility, and incompatibility with other polysaccharides. To address these limitations, this study investigated the effects of different concentrations (0.05-0.

Insight into the relationship between cell wall, intracellular starch structure and physicochemical properties of cassava cells from cassava varieties.

Cassava cell flour can expand the food industrial availability of cassava resources. In this study, cassava cells were isolated from eight cassava varieties to analyze the composition, structure, and physicochemical properties. The smaller particle size in CS4 led to the lowest swelling power and viscosity, which further reduced the modulus (G', G") and shear stress of the cassava cell gel.

A stand-alone and point-of-care electrochemical immuno-device for Salmonella typhimurium testing.

The rapid development of accurate and point-of-care diagnostic tools for foodborne diseases has made a massive impact in global health. Salmonella typhimurium (S. typhimurium) exemplifies an enteric pathogen, being a gram-negative bacteria responsible for several gastrointestinal and systemic illnesses.

ADRB2 serves as a novel biomarker and attenuates alcoholic hepatitis via the SIRT1/PGC-1α/PPARα pathway: integration of WGCNA, machine learning and experimental validation.

Background: Alcoholic hepatitis is a severe inflammatory liver disease. In recent years, the incidence of AH has been on the rise, leading to an increasingly severe disease burden. Currently, there is a lack of specific biomarkers for the diagnosis and prognosis of AH in clinical practice.

Characteristics of the effects of Polygonati Rhizoma on gut microbiota and metabolites in vitro associated with poor dietary habits in pregnant women.

Poor dietary habits have been associated with dysbiosis and microbial imbalance in pregnant women. Such imbalances can pose health risks during pregnancy. This study aimed to explore the impact of Polygonati Rhizoma on the gut microbiota of pregnant women through In vitro simulated fermentation.

Visible light-induced PPh/MI-promoted δ-C(sp)-H chlorination and cyclization with -chloro-arylsulfonamides EDA complexes.

Triphenylphosphine, iodide and -chloro-arylsulfonamides could generate amidyl radicals EDA (Electron Donor-Acceptor) complexes under visible light irradiation, and this strategy enables the synthesis of valuable δ-chloro-arylsulfonamide and -arylsulfonylpyrrolidine motifs in moderate yields. This blue LED-induced method utilizes more readily available reagents, providing advantages in terms of cost efficiency, broad substrate scope, and functional-group compatibility.

Advancing the Validation of the Enrichment-Enhanced Detection Strategy with Au Nanoclusters for AChE Detection.

High-sensitivity fluorescent probes provide a powerful tool for understanding life processes and functioning mechanisms. Therefore, the development of a universal strategy to optimize probes holds substantial importance. Herein, we developed a novel strategy for common probe upgrades: rather than simply pursuing a higher fluorescence intensity of the probe itself, we tried to promote the detection sensitivity by enhancing the probe-substrate interactions.

Discovery of the First BRD4 Second Bromodomain (BD2)-Selective Inhibitors.

Pan-BD2 inhibitors have been shown to retain an antileukemia effect and display less dose-limiting toxicities than pan-BET inhibitors. However, it is necessary to consider the potential off-target toxicity associated with the inhibition of four BET BD2 proteins. To date, no BRD4 BD2 domain selective inhibitor has been reported.

Nutrient Content Prediction and Geographical Origin Identification of Bananas by Combining Hyperspectral Imaging with Chemometrics.

The nutritional quality of bananas and their geographical origin authenticity are very important for trade. There is an urgent need for rapid, non-destructive testing to improve the origin and quality assurance for importers, distributors, and consumers. In this study, 99 banana samples from a range of producing countries were collected.

Replacing traditional coffee appraisers with inductively coupled plasma - mass spectrometry (ICP-MS): From manual sensory evaluation to scientific analysis.

Scientific development provides opportunities to replace many traditional manual methods to achieve more accurate results and higher efficiency. To scientifically ascertain the geographical origin of coffee, this study develops a method for the rapid determination of 16 inorganic elements in coffee using microwave digestion combined with the ICP-MS internal standard method. Principal component analysis (PCA), Fisher discriminant analysis (FDA), and Partial least squares discriminant analysis (PLS-DA) are employed to analyze 40 coffee samples from three production areas of Pu'er, Baoshan, and Wanning.

Biosensors with vancomycin and polymetallic metal-organic frameworks for colorimetric-fluorescent dual-mode detection and sterilization of bacteria.

The development of a versatile platform for bacterial assay and elimination is urgently needed due to the danger that bacteria pose to human life. Here, we synthesized a trimetallic deposition and horseradish peroxidase (HRP)-embedded porous coordination network-224 hybrid nanozymes (PCN-224 @AuPdPt@HRP) with outstanding peroxidase activity and fluorescence quenching ability. On this basis, we designed a dual recognition strategy-driven colorimetric-fluorescence dual-mode detection platform using Listeria monocytogenes as a pattern analyte.

Effects of Essential Oil Blends Supplementation on Growth Performance, Meat Physiochemical Parameters, Intestinal Health and Lipid Metabolism of Weaned Bamei Piglets.

The benefits of plant essential oils (EO) on the health of animals have been frequently reported, but their alteration of lipid metabolism in obese pigs has yet to be explored. This study aimed to assess the impact of EO blends (oregano, cinnamon and lemon oils) on growth performance, meat physicochemical parameters, intestinal health and lipid metabolism in the small intestine of weaned Bamei (a kind of obese-type pig) piglets. One hundred and forty-four male 60-day-old weaned Bamei piglets were randomly assigned to three groups of six replicates each: CON (basal diet), T1 (basal diet + 250 mg/kg EO), and T2 (basal diet + 500 mg/kg EO) over 28 days.

Regioselective functionalization of cellulose nanomaterial for advanced application.

Cellulose nanomaterials (CNMs) with their remarkable properties and abundant natural sources have emerged as a versatile platform for material science. However, their widespread adoption to develop novel applications often hinges on precise control over their surface chemistry. Regioselective functionalization, i.

Esterified starches enhance short-term satiety in mice structural and physicochemical alterations.

Starch is the main carbohydrate in the human diet, of which resistant starch (RS) has positive effects on satiety. This study examined the impact of esterified starch with varying degrees of substitution on short-term satiety in mice. Three forms of esterified starch were investigated: phosphorylated starch (SP), acetylated starch (SA), and octenylsuccinic acid starch (OSA).

Thermally processed rice starch impacts glucose homeostasis in mice to different degrees via disturbing gut microbial structure and intestinal barrier function.

Long-term intake of thermally processed starch-based foods may impact glucose homeostasis, but the consistency of the effects of various thermal treatments and the reasons are not clear. In this study, thermal treatments, especially boiling, damaged the crystal structure and inter-molecular hydrogen bonds of starch-based blends, thus decreasing the structural order and stability. These thermally treated starch-based blends increased the appetite of mice, promoted food digestion, and enhanced postprandial glucose response.

Hydrovoltaic-Photoelectric Coupling Strategy Triggered a Robust Output Signal for High-Performance Self-Powered Electrochemical Sensing.

Hydrovoltaic self-powered electrochemical sensors hold significant potential for constructing wearable, portable, and real-time detection devices, but the low output signal due to the slow phase transition rate of water molecules and the intricate nature of integration limits their applications. In this work, a hydrovoltaic-photovoltaic coupling effect-enhanced self-powered electrochemical sensor was prepared by combining zinc oxide (ZnO) nanowire arrays with cerium-organometallic framework (Ce-MOF) materials, which greatly improved the electrical output of self-powered electrochemical systems and provided a new detection strategy for an efficient self-powered electrochemical sensing system. The heterojunction constructed by ZnO arrays and Ce-MOF could generate a built-in electric field under the action of light irradiation and promote the separation of the photocarriers.

Singlet oxygen storage and controlled release for improving photodynamic therapy against hypoxic tumor.

Photodynamic therapy (PDT) is considered to be a promising tumor treatment method due to its non-invasiveness and low risk. However, there are two factors that affect the efficacy of this therapy. One is the light source and the other is the tumor hypoxia.

Synthesized CuO-PEI-JE with 3D open-cell structure as an efficient heterogeneous activator of peroxodisulfate for phenol degradation.

The core of heterogeneous catalytic systems is to design high-performance heterogeneous catalysts. In the present study, CuO-PEI-JE was synthesized via in situ precipitation of CuO on the 3D structure of PEI-JE prepared by a cross-linking method. It possessed a macro-size and 3D open-cell structure, being easily separated and having super-performance for PDS activation.

A fully integrated wireless microfluidic immunosensing system for portable monitoring of Staphylococcus aureus.

The advanced devices that function fully without the need for external accessories are regarded as a pinnacle goal in the design and construction of modern ones. Staphylococcus aureus (S. aureus), a prominent human pathogen, is responsible for causing a wide variety of infections and chronic diseases.

Fine mapping of QGPC.caas-7AL for grain protein content in bread wheat.

A major stable QTL, QGPC.caas-7AL, for grain protein content of wheat, was narrowed down to a 1.82-Mb inter on chromosome 7AL, and four candidate genes were predicated.

Boosting the Structural and Electrochemical Stability of Chloride-Ion-Conducting Perovskite Solid Electrolytes by Alkali Ion Doping.

The use of chloride-based solid electrolytes derived from Lewis acid‒base reactions enables the construction of various new rechargeable batteries, such as chloride ion batteries (CIBs). However, a critical problem with these electrolytes is their poor stability under low-temperature, moist, or electrochemical conditions, which can lead to deterioration of the phase structure and a loss of ion conduction. Herein, the robust cubic structure of tin-based perovskite chloride-a chloride ion conductor-is achieved by alkali ion doping at the tin site via direct mechanical milling.