Silencing of lncRNA Gm26917 Attenuates Alveolar Macrophage-mediated Inflammatory Response in LPS-induced Acute Lung Injury Via Inhibiting NKRF Ubiquitination.

The inflammatory response mediated by alveolar macrophages plays a crucial role in the development of acute lung injury. Numerous studies have reported that lncRNAs are highly expressed in acute lung injury in mouse models and cell lines, and acute lung injury (ALI) can be effectively alleviated by targeting these lncRNAs. The aim of this study was to explore the mechanism by LncRNA Gm26917 regulates the inflammatory response in alveolar macrophages during acute lung injury mouse model.

Cryo-EM reveals cholesterol binding in the lysosomal GPCR-like protein LYCHOS.

Cholesterol plays a pivotal role in modulating the activity of mechanistic target of rapamycin complex 1 (mTOR1), thereby regulating cell growth and metabolic homeostasis. LYCHOS, a lysosome-localized G-protein-coupled receptor-like protein, emerges as a cholesterol sensor and is capable of transducing the cholesterol signal to affect the mTORC1 function. However, the precise mechanism by which LYCHOS recognizes cholesterol remains unknown.

Transcriptome and DNA methylation analysis of the goat pineal gland during puberty.

Previous studies have confirmed that methylation regulates gene transcription in the hypothalamus-pituitary-gonadal axis during puberty initiation, but little is known about the regulation of DNA methylation on gene expression in the pineal gland. To screen pineal gland candidate genes related to the onset of goat puberty and regulated by genome methylation, we collected pineal glands from prepubertal and pubertal female goats, then, determined the DNA methylation profile by whole genome bisulfite sequencing and the transcriptome by RNA sequencing on Illumina HiSeqTM2500. We analyzed differentially expressed genes between the Pre group and Pub group using the DESeq2 software (version 1.

Optimal dose and type of exercise improve walking velocity in adults with Parkinson's disease: a systematic review and Bayesian network meta-analysis.

To examine the dose-response relationship between specific types of exercise for improving walking velocity in Parkinson's disease (PD). This systematic review and network meta-analysis included searches of PubMed, Medline, Embase, PsycINFO, Cochrane Library, and Web of Science were searched from inception until February 18th, 2024. Data analysis was performed using R software with the MBNMA and RJAGS packages.

NKAPL facilitates transcription pause-release and bridges elongation to initiation during meiosis exit.

Transcription elongation, especially RNA polymerase II (Pol II) pause-release, is less studied than transcription initiation in regulating gene expression during meiosis. It is also unclear how transcription elongation interplays with transcription initiation. Here, we show that depletion of NKAPL, a testis-specific protein distantly related to RNA splicing factors, causes male infertility in mice by blocking the meiotic exit and downregulating haploid genes.

Manipulating Charge Distribution at Organic-inorganic Interface via Optimizing Substituents for Sustainable Water Electrolysis.

The space charge effect induced by high-quality heterojunctions is essential for efficient electrocatalytic processes. Herein, we delicately manipulate intermolecular charge transfer by modifying substituents (-g = -CH3, -H, -NO2) with various electron donating/withdrawing capabilities in CoPc-g/CoS organic-inorganic heterostructures. CoPc-CH3, as a typical electron donor, transfers more electrons to CoS due to the presence of -CH3, forming the strongest space electric field and thus regulating the dual active sites at the interface.

Serum autoantibody-based biomarkers for prognosis in early-stage lung cancer patients with surgical resection.

Background: Lung cancer is the cancer with the highest morbidity and mortality in the world. With the increasing diagnosis rate of patients with early-stage lung cancer, surgery treatment becomes an option for more patients. However, there is a lack of effective indicators to assess the risk of recurrence after lung cancer surgery.

Hydrochemical fingerprints and health risk assessment of groundwater contamination in the Bohai Sea region, China.

Seawater intrusion and human activities have significantly impacted coastal groundwater quality in many regions worldwide. This study systematically assessed groundwater chemistry, its suitability for drinking and irrigation (sample size, n = 3034), and exposure risks (n = 2863) across three key sub-regions of the Bohai Sea area: Bohai Bay, Liaodong Bay, and Laizhou Bay. Significant seasonal variations observed in groundwater chemistry at different depths in Bohai Bay region, with severe contamination from salinity-alkalinity and nitrogen-fluoride.

Analysis of potential human accumulation differences and mechanisms of environmental new flame retardants: Based on in vitro experiments and theoretical calculations.

Hundreds of new flame retardants (NFRs) are widely used, causing environmental pollution and threating human health. In this study, based on the interaction of NFRs and human serum albumin (HSA), we assessed the differences in potential human accumulation of 8 NFRs including 1,2-Dibromo-4-(1,2-dibromoethyl)cyclohexane (TBECH), tetrabromobisphenol A bis(dibromopropyl ether) (TBBPA-DBPE), 2,4,6-tribromophenol (TBP), pentabromophenol (PBP), tri-n-butyl phosphate (TnBP), triphenyl phosphate (TPP), Tri(2-chloroethyl) phosphate (TCEP), and Tri(1,3-dichloro-2-propyl) phosphate (TDCP). All NFRs could bind to HSA and cause slight damage to its structure, suggesting their potential human accumulation ability.

Unpacking the green potential: How does supply chain digitalization affect corporate carbon emissions? - Evidence from supply chain innovation and application pilots in China.

The impact of supply chain digitalization (SCD) on carbon dioxide emissions is an emerging area of research, particularly in China, which is the world's largest carbon emitter. This study uses micro-level data on listed companies from 2010 to 2021 to systematically verify the impact and mechanism of SCD on corporate carbon emissions (CCE) through the difference-in-differences model. We determined that SCD can significantly reduce CCE and its implementation path involves three aspects: promoting technological innovation, reducing financing constraints, and increasing market attention.

Adiponectin regulates proliferation and differentiation of chicken skeletal muscle satellite cells via ERK1/2 and p38 signaling pathways.

Skeletal muscle satellite cells (SMSCs) are critical for postnatal skeletal muscle growth and regeneration. Adiponectin plays a pivotal role in regulating muscle glucose uptake and fatty acid metabolism. However, its function in the proliferation and differentiation of chicken SMSCs remains poorly understood.

Anti-colorectal cancer actions of Glycyrrhiza uralensis Fisch. and its underlying mechanism via HPLC integration and network pharmacological approaches.

Background: The therapeutic and prognostic outcomes for colorectal cancer (CRC) remain unsatisfactory. Among multiple reported bioactive functionalities of Glycyrrhiza uralensis Fisch. one vital recently reported activity is its therapeutic role against numerous cancers but limited information is available related to its underlying key mechanisms and therapeutically active ingredients, especially against CRC treatment.

Glaesserella parasuis serotype 5 promotes pyroptosis via degrading Caveolin-1 in 3D4/21 cells.

Glaesserella parasuis (G. parasuis) is an important pathogen, which can cause systemic inflammatory response in pigs and bring huge economic losses to the global swine industry. G.

IFN-mediated lncRNA-ISL promotes SVV infection through G1P3.

lncRNAs play important regulatory roles in almost every aspect of physiological processes. However, the mechanisms by which animal-encoded lncRNAs regulate the interaction of viral infection with host antiviral immunity are unknown. To explore the mechanisms of lncRNA regulation of SVV infection and interferon responses.

Sensitive and Deep Coverage Phosphoproteome Detection Method Reveals Spleen as a More Sensitive Organ than Liver in Early Detection of Liver Fibrosis-Related Signaling Protein Dysregulation.

Although cathepsin S is transported from the spleen to the liver, where it cleaves collagen XVIII to produce endostatin and plays a critical role in the onset of early liver fibrosis, the relationship between liver fibrosis and spleen function remains underexplored. Given the roles of phosphorylation in disease, understanding its regulatory mechanism in early liver fibrosis is crucial. Despite advances in mass spectrometry enhancing phosphoproteomics, its application is limited by small clinical samples and subtle protein changes.

The transcription factor SlLBD40 regulates seed germination by inhibiting cell wall remodeling enzymes during endosperm weakening.

Uniform seed germination is crucial for consistent seedling emergence and efficient seedling production. In this study, we identified a seed-expressed protein in tomato (Solanum lycopersicum), lateral organ boundaries domain 40 (SlLBD40), that regulates germination speed. CRISPR/Cas9-generated SlLBD40 knockout mutants exhibited faster germination due to enhanced seed imbibition, independent of the seed coat.

LARP3 inhibits the apoptosis of hepatocellular carcinoma via the ROS/PI3K/c-Fos axis.

Primary hepatocellular carcinoma (PHC) is the sixth most common cancer and the third leading cause of cancer death worldwide. Hepatocellular carcinoma (HCC) accounts for 75%-85% of PHC. LARP3 is aberrantly expressed in multiple cancers.

Aucubin Promotes BMSCs Proliferation and Differentiation of Postmenopausal Osteoporosis Patients by Regulating Ferroptosis and BMP2 Signalling.

Postmenopausal osteoporosis (PMOP) is a chronic systemic bone metabolism disorder. Promotion in the patterns of human bone marrow mesenchymal stem cells (hBMSCs) differentiation towards osteoblasts contributes to alleviating osteoporosis. Aucubin, a natural compound isolated from the well-known herbal medicine Eucommia, was previously shown to possess various pharmacological effects.

Aberrant acetylation caused by a CDYL splicing mutation contributes to thin mid-piece related asthenoteratozoospermia and male infertility.

Objectives: Acetylated tubulin is a hallmark of flagellar stability in spermatozoa, and studies have demonstrated the ability of CDYL to function as a tubulin acetyltransferase in spermatozoa. Of note, germline conditional knockout of Cdyl can lead to asthenoteratozoospermia and infertility in male mice. However, the role of CDYL gene in human fertility remains uncharacterized.

Neural underpinnings of a two-phase memory suppression process in the neural response to self-related and observed perspective views.

Individuals often actively suppress intrusive memories to alleviate the distress they cause and maintain mental well-being. However, those with post-traumatic stress disorder (PTSD) often exhibit difficulties particularly in inhibiting or suppressing negative memories compared to individuals without PTSD. These memories can involve a physical threat either to the individual themselves or to others.

Genome-wide analyses of the NAC transcription factor gene family in provide valuable insights into the natural process of leaf senescence.

is a deciduous shrub or small tree. It is a popular ornamental plant because of its beautiful leaves, which change colour in autumn. This study revealed 116 genes within the genome of .

Serum metabolites and risk of aortic dissection: a two-sample Mendelian randomization study.

Purpose: Acute aortic dissection is a serious cardiovascular emergency with a high mortality rate. Its pathogenesis is complex and remains unclear. This study aimed to assess the connection between the levels of genetically predicted circulating metabolites and the risk of aortic dissection.

Defects Calculation and Accelerated Interfacial Charge Transfer in a Photoactive MOF-Based Heterojunction.

Photocatalytic hydrogen production is currently considered a clean and sustainable route to meet the energy and environmental issues. Among, heterojunction photocatalysts have been developed to improve their photocatalytic efficiency. Defect engineering of heterojunction photocatalysts is attractive due to it can perform as electron trap and change the band structure to optimize the interfacial separation rate of photogenerated electron-hole pairs.

Enhancing Photocatalytic CORR by Modulating the Active Sites of COF-Based Catalysts.

The catalytic conversion of CO into valuable chemicals using metalized covalent organic frameworks (COFs) as catalysts is a promising method for reducing atmospheric CO levels. Herein, a aldehyde-amine COF (TAPT-Tp) at room temperature and pressure and their metallized results is synthesized, Ni-TAPT-Tp and Ti-TAPT-Tp. The photocatalytic results indicate that the CO to CO reduction rate is 6182.

In-Situ Formation of Three-Dimensional Network Intrinsic Microporous Ladder Polymer Membranes with Ultra-High Gas Separation Performance and Anti-Trade-Off Effect.

The global quest for clean energy and sustainable processes makes advanced membrane extremely attractive for energy-intensive industrial gas separations. Here, we disclosed a series of ultra-high-performance gas separation membranes (PIM-3D-TB) from novel network polymers of intrinsic microporosity (PIM) that combine the advantages of solution processible PIM and small pore size distribution (PSD) of porous organic polymers (POP), which was synthesized by in situ copolymerization of triptycene-2,6-diamine as linear part and triptycene-2,6,13(14)-triamine (TTA) as crosslinker. The resulting PIM-3D-TB membranes demonstrated outstanding separation properties that outperformed the latest trade-off lines for H/CH and O/N.