Construction of scFv Antibodies against the Outer Loops of the Microsporidium ATP/ADP-Transporters and Selection of the Fragment Efficiently Inhibiting Parasite Growth.

Traditional sanitation practices remain the main strategy for controlling infections caused by microsporidia . This actualizes the development of new approaches to increase the silkworm resistance to this parasite. Here, we constructed a mouse scFv library against the outer loops of ATP/ADP carriers and selected nine scFv fragments to the transporter, highly expressed in the early stages of the parasite intracellular growth.

SSR Variability of Stem Rust Pathogen on Spring Bread Wheat in Russia.

Wheat stem rust, caused by f. sp. , which used to be a harmful disease of winter wheat in the southern part of Russia, has been largely affecting the yield of spring bread wheat in the territories of the temperate climate zone since 2009.

The Effect of Bean Seed Treatment with Entomopathogenic Fungus on Soil Microarthropods (Acari, Collembola).

The treatment of agricultural crops with entomopathogenic fungi may disturb the structure of soil microarthropod communities, which can have an adverse impact on soil fertility and, ultimately, on the yield. The effect of the treatment of broad bean ( L.) seeds, with the entomopathogenic fungus , on the abundance and community structure of soil microarthropods in the rhizosphere was assessed in different phases of plant vegetation in a two-year experiment.

A new genus and species of the aphid parasitoids (Hymenoptera: Braconidae: Aphidiinae) from Baltic amber.

A new genus and species, of aphid parasitoid, Protoctonus groehni Davidian, gen. et sp. nov.

Comparative Analysis of Epicuticular Lipids in and : A Possible Role in Susceptibility to Entomopathogenic Fungi.

Cuticular lipids protect insects from desiccation and may determine resistance to fungal pathogens. Nonetheless, the trade-off between these lipid functions is still poorly understood. The migratory locust and the Italian locust have dissimilar hygrothermal preferences: inhabits areas near water bodies with a reed bed, and exploits a wide range of habitats and prefers steppes and semideserts with the predominance of sagebrushes.

Draft Genome Sequences of Prototrophic and Biotin-Auxotrophic Fusarium langsethiae Strains Isolated from an Oat Grain in the Northern Region of Russia.

Fusarium langsethiae is a suspected plant-pathogenic fungus causing cereal contamination with trichothecene mycotoxins. Here, we report the genome sequences of two strains, MFG217701 (a prototroph) and MFG217702 (a biotin auxotroph), isolated from a grain of oat harvested in Russia.

Distinction of Sect. Strains among Other Fungi from Cereals.

Species of the genus are ubiquitous and frequently isolated from various plants, including crops. There are two phylogenetically and morphologically close sections: the relatively well-known and the rarely mentioned . Currently, the latter includes at least seven species that are less studied and sometimes misidentified.

species infecting sunflower () in Russia, with the description of two new species.

Phomopsis stem canker is economically important sunflower disease that caused by multiple species. Recent investigations resulted in the resolution that there are at least 13 species that can infect sunflower. A comprehensive analysis of the biodiversity and geographic distribution of species in Russia, particularly those that infect sunflower, has not been undertaken.

Heterologous expression of scFv fragment against Vairimorpha (Nosema) ceranae hexokinase in Sf9 cell culture inhibits microsporidia intracellular growth.

Secretion of hexokinase (HK) by microsporidia into infected cells suggests an important role for this enzyme for the intracellular development of parasites. To verify whether the expression of HK-specific antibodies in the host cell cytoplasm can suppress the growth of microsporidia, we constructed an immune library of recombinant scFv fragments against the enzyme of the honey bee pathogen Vairimorpha (Nosema) ceranae (VcHK) with a representativeness of about 5 million bacterial transformants. Two variants of VcHK-specific recombinant antibodies were selected by library panning and expressed in lepidopteran Sf9 cell line.

A new species of the genus Dendrolasiophilus Nomura, 2010 from the south of the Russian Far East (Coleoptera, Staphylinidae, Pselaphinae).

Dendrolasiophilus subitus sp. nov. from the Russian Far East is described and illustrated.

Monograph on the Cillaeinae (Coleoptera: Nitidulidae) from the Australian Region with comments on the taxonomy of the subfamily.

General review of the taxonomy and composition of the subfamily Cillaeinae with description of Conotelini trib. nov. (type genus: Conotelus Erichson, 1843 with some necessary notes on the position and rank of some recent and fossil taxa are given.

A New Genus of Tachydromiinae from South Africa and Turkmenistan (Diptera: Empidoidea: Hybotidae).

gen. nov. is described with the inclusion of two new species from South Africa (.

Current State of Hyperspectral Remote Sensing for Early Plant Disease Detection: A Review.

The development of hyperspectral remote sensing equipment, in recent years, has provided plant protection professionals with a new mechanism for assessing the phytosanitary state of crops. Semantically rich data coming from hyperspectral sensors are a prerequisite for the timely and rational implementation of plant protection measures. This review presents modern advances in early plant disease detection based on hyperspectral remote sensing.

A prebreeding study of introgression spring bread wheat lines carrying combinations of stem rust resistance genes, Sr22+Sr25 and Sr35+Sr25.

The Sr22, Sr35, and Sr25 genes attract the attention of bread wheat breeders with their effectiveness against Puccinia graminis f. sp. tritici race Ug99 and its biotypes.

Resistance of oat breeding lines to grain contamination with Fusarium langsethiae and T-2/HT-2 toxins.

Fusarium disease of oats reduces yield quality due to decreasing germination that is caused by the contamination of grain with mycotoxins produced by Fusarium fungi. The aim of this study was to characterize the resistance of naked breeding lines of oats to fungal grain infection and to contamination with T-2 and HT-2 toxins. Thirteen naked oat breeding lines and two naked varieties, Nemchinovsky 61 and Vyatskiy, as well as a husked variety Yakov, were grown under natural conditions in the Nemchinovka Federal Research Center in 2019-2020.

Complex resistance of spring and winter bread wheat lines to biotic and abiotic stresses.

An original initial material of spring and winter bread wheat with group resistance to stem and leaf rust was developed using new donors of resistance to stem rust: winter soft wheat GT 96/90 (Bulgaria) and accession 119/4-06rw with genetic material of the species Triticum migushovae and (Aegilops speltoides and Secale cereale), respectively, a line of spring wheat 113/00i-4 obtained using the species Ae. triuncialis and T. kiharae, as well as spring accession 145/00i with genetic material of the species Ae.

[New genetic tools for plant defense against parasitic nematodes].

. Nematodes belong to economically important pests. Here we reviewed the recent data on molecular mechanisms of plant resistance to cyst and gall nematodes including the most devastating Globodera rostochiensis, G.

Potato spindle tuber viroid.

Viroids belong to a very interesting class of molecules attracting researchers in phytopathology and molecular evolution. Here we review recent literature data concerning the genetics of Potato spindle tuber viroid (PSTVd) and the mechanisms related to its pathological effect on the host plants. PSTVd can be transmitted vertically through microspores and macrospores, but not with pollen from another infected plant.

Analysis of Host-Specific Differentiation of in the South and North-West of the European Part of Russia.

Yellow (stripe) rust, caused by Westend. (), is a major disease of cereals worldwide. We studied virulence phenotypes on , , and triticale in three geographically distant regions of the European part of Russia (Dagestan and Krasnodar in North Caucasus, and Northwest) with different climate and environmental conditions.

Fungal Metagenome of Chernevaya Taiga Soils: Taxonomic Composition, Differential Abundance and Factors Related to Plant Gigantism.

The Chernevaya taiga of Western Siberia is a unique and complex ecosystem, distinguished by the unusually large sizes of herbaceous plants, the reasons for which are poorly understood. Here, we explored the fungal diversity of the Chernevaya taiga soils in the Tomsk regions of Western Siberia in comparison with other soil types. The soil biomes of Chernevaya taiga and the control regions were investigated using Illumina ITS rRNA sequencing, and taxonomic analysis revealed a predominance of fungal phyla in the different soils.

Wild grasses as the reservoirs of infection of rust species for winter soft wheat in the Northern Caucasus.

Common winter wheat is the main grain crop cultivated in the North Caucasus. Rust disease damage is one of the factors limiting wheat productivity. There are three species of rust in the region: leaf (Puccinia triticina), stem (P.

Draft Genome Sequence of Pseudomonas brassicacearum Strain UTMN3, a Biological Control Agent from the Rhizosphere of Pisum sativum.

Here, we announce and describe the draft genome sequence of Pseudomonas brassicacearum UTMN3, which contains 40 contigs comprising 6,658,810 bp, with a GC content of 60.9%. The genome contains 5,825 protein-coding genes and 65 RNA-coding genes.

Fusarium head blight in the Russian Far East: 140 years after description of the 'drunken bread' problem.

The first appearance of Fusarium head blight (FHB)-and the beginning of scientific research of this disease-occurred the Far East region of Russia at the end of the 19th century. In the summer of 2019, in the Amur region, which comprises 60-70% of grain production in the Russian Far East, flooding caused a state of emergency. The quality of wheat and barley grains grown under natural conditions of FHB outbreaks, including grain infection, fungal species composition, DNA content of and chemotypes, and the presence of various mycotoxins, was studied.