The study focused on a diffusion chamber (DC) system combined with a scaffold to culture cells in vivo, reducing the risk of contamination.
The experiment involved using a PLGA sponge and rat bone marrow cells, which were placed inside a DC and implanted in the abdominal cavities of rats.
After four weeks, the results showed the formation of fibrous and calcified tissue around the sponge, demonstrating that the PLGA sponge effectively supported three-dimensional tissue formation in vivo with minimal need for special technologies post-grafting.