Potential of a newly isolated lytic bacteriophage to control Pseudomonas coronafaciens pv. garcae in coffee plants: Molecular characterization with in vitro and ex vivo experiments.

Traditionally, control of coffee plant bacterial halo blight (BHB) caused by the phytopathogen Pseudomonas coronafaciens pv. garcae (Pcg) involves frequent spraying of coffee plantations with non-environmentally friendly and potentially bacterial resistance-promoting copper products or with kasugamycin hydrochloride. In this study we report a leap forward in the quest for a new ecofriendly approach, characterizing (both physicochemically and biologically) and testing both in vitro and ex vivo a new lytic phage for Pcg.

Polyphenolic Profile and Antioxidant Activity of Whole Grape Juices from and Brazilian Hybrid Grapes in Two Training Systems.

The phenolic profile and antioxidant activity of whole grape juices from and Brazilian hybrids in two training systems were analyzed. Genotypes of ('Bordô' and 'Isabel') and Brazilian hybrids ('IAC 138-22 Máximo' and 'BRS Violeta') were grafted onto the rootstock 'IAC 766 Campinas' (106-8 'Mgt' × ) and trained on low and high trellis. After harvest, the grapes were destemmed and the berries macerated in a roller crusher.

Black Crust Complex: Influence of Temperature and Period of Wetness on the Development of Fungi in .

The objective of this work was to evaluate the development of sp. and its asexual form, sp., under different environmental conditions in the rubber tree ().

Mix-method toolbox for monitoring greenhouse gas production and microbiome responses to soil amendments.

In this study, we adopt an interdisciplinary approach, integrating agronomic field experiments with soil chemistry, molecular biology techniques, and statistics to investigate the impact of organic residue amendments, such as vinasse (a by-product of sugarcane ethanol production), on soil microbiome and greenhouse gas (GHG) production. The research investigates the effects of distinct disturbances, including organic residue application alone or combined with inorganic N fertilizer on the environment. The methods assess soil microbiome dynamics (composition and function), GHG emissions, and plant productivity.

Differential amplification of the subtelomeric satellite DNA JcSAT1 in the genus Jatropha L. (Euphorbiaceae).

Satellite DNAs (satDNAs) are highly repetitive sequences that occur in virtually all eukaryotic genomes and can undergo rapid copy number and nucleotide sequence variation among relatives. After chromosomal mapping of the satDNA JcSAT1, it was found a large accumulation at subtelomeres of Jatropha curcas (subgenus Curcas), but an absence of these monomers in J. integerrima (subgenus Jatropha).

CRISPR technology towards genome editing of the perennial and semi-perennial crops citrus, coffee and sugarcane.

Gene editing technologies have opened up the possibility of manipulating the genome of any organism in a predicted way. CRISPR technology is the most used genome editing tool and, in agriculture, it has allowed the expansion of possibilities in plant biotechnology, such as gene knockout or knock-in, transcriptional regulation, epigenetic modification, base editing, RNA editing, prime editing, and nucleic acid probing or detection. This technology mostly depends on tissue culture and genetic transformation/transfection protocols, which sometimes become the major challenges for its application in different crops.

Carbon and Nutrients from Organic Residues Modulate the Dynamics of Prokaryotic and Fungal Communities.

Inputs of carbon (C) and nutrients from organic residues may select specific microbes and shape the soil microbial community. However, little is known about the abiotic filtering of the same residues with different nutrient concentrations applied to the soil. In our study, we explored how applying organic residue, vinasse, as fertilizer in its natural state (V) versus its concentrated form (CV) impacts soil microbiota.

Multi-experiment assessment of soil nitrous oxide emissions in sugarcane.

Unlabelled: Soil nitrous oxide (NO) fluxes comprise a significant part of the greenhouse gas emissions of agricultural products but are spatially and temporally variable, due to complex interactions between climate, soil and management variables. This study aimed to identify the main factors that affect NO emissions under sugarcane, using a multi-site database from field experiments. Greenhouse gas fluxes, soil, climate, and management data were obtained from 13 field trials spanning the 2011-2017 period.

Newly isolated phages preying on Pseudomonas syringae pv. garcae: In vitro and ex vivo inactivation studies in coffee plant leafs.

Coffee canker, or bacterial halo blight (BHB) of coffee, is a disease caused by the phytopathogenic bacterium Pseudomonas syringae pv. garcae (Psg), having been found for the first time in 1955, in the Garça region (State of São Paulo), and which has stood out in the Brazilian coffee plantations in recent years, leading to severe economic losses that seriously affect coffee trade. The treatments available are still scarce, involving frequent spraying of coffee plantations with either copper derivatives or the antibiotic kasugamycin.

New trends in sugarcane fertilization: Implications for NH volatilization, NO emissions and crop yields.

Recycling nutrients helps to reduce the environmental impact of agriculture and contributes to alleviating the effects of global climate change. A recent trend in sugarcane cultivation is the application of concentrated vinasse (CV) combined with fertilizers into an organo-mineral formulation to improve logistics, reduce costs and foster the circular economy. However, the implications of the application of such organo-mineral formulation in sugarcane fields are unclear.

Marker-assisted introgression of wild chromosome segments conferring resistance to fungal foliar diseases into peanut ( L.).

Introduction: Fungal foliar diseases can severely affect the productivity of the peanut crop worldwide. Late leaf spot is the most frequent disease and a major problem of the crop in Brazil and many other tropical countries. Only partial resistance to fungal diseases has been found in cultivated peanut, but high resistances have been described on the secondary gene pool.

ROOT parameters of sugarcane and soil compaction indicators under deep strip tillage and conventional tillage.

Soil tillage and agricultural traffic generate changes in soil physical attributes and affect the growth of the roots. This study evaluates the impact of system soil tillage on compaction and sugarcane root growth. The experiment was carried out on a Rhodic Kandiudox with two soil tillages (Deep Strip Tillage and Conventional Tillage) and two positions (beds or traffic lane and no traffic lane), totaling four treatments (DST-beds + no traffic lane, DST-traffic lane, CT-no traffic lane and CT-traffic lane).

Taxonomically Restricted Genes Are Associated With Responses to Biotic and Abiotic Stresses in Sugarcane ( spp.).

Orphan genes (OGs) are protein-coding genes that are restricted to particular clades or species and lack homology with genes from other organisms, making their biological functions difficult to predict. OGs can rapidly originate and become functional; consequently, they may support rapid adaptation to environmental changes. Extensive spread of mobile elements and whole-genome duplication occurred in the group, which may have contributed to the origin and diversification of OGs in the sugarcane genome.

Phytotonic effects of thiamethoxam on sugarcane managed with glyphosate as a ripener.

Background: Thiamethoxam and glyphosate are widely used in sugarcane production as an insecticide and ripener, respectively. In this study, the potential of these chemical products to also elicit phytotonic effects and enhance the physiological development and yield of sugarcane was evaluated. In field experiments, thiamethoxam and glyphosate were applied to sugarcane individually or in combination, and the effects of these chemical management strategies on sugarcane biometric and technological parameters were assessed.

Monitoring Colonization and Reproduction in Different Rubber Tree Clones.

Anthracnose, caused by fungi of the genus , is present in the major rubber tree crop areas in Brazil, especially in São Paulo, Mato Grosso do Sul, Paraná, Minas Gerais, Espírito Santo, and northern states. This disease can affect different tissues of the rubber tree, leading to production losses. Thus, a better understanding of the pathosystem x rubber tree can provide evidence to subsequent epidemiological research and phytosanitary management studies of this disease in the field.

Dynamics of Macronutrient Uptake and Removal by Modern Peanut Cultivars.

The productive potential of new peanut cultivars has increased over the years in relation to old cultivars, especially when compared with ones with upright growth habit. Thus, the requirement for macronutrients for these new cultivars may also have increased, making the existing fertilizer recommendation tables obsolete, thus increasing the need for further studies measuring the real macronutrient requirements of these new peanut cultivars. Our study aimed to evaluate the growth patterns and the macronutrient absorption rate throughout the biological cycle of three modern runner peanut cultivars, as well as the potential for producing dry matter, pods, and kernels, and their respective macronutrient accumulations.

Genome-wide approaches for the identification of markers and genes associated with sugarcane yellow leaf virus resistance.

Sugarcane yellow leaf (SCYL), caused by the sugarcane yellow leaf virus (SCYLV) is a major disease affecting sugarcane, a leading sugar and energy crop. Despite damages caused by SCYLV, the genetic base of resistance to this virus remains largely unknown. Several methodologies have arisen to identify molecular markers associated with SCYLV resistance, which are crucial for marker-assisted selection and understanding response mechanisms to this virus.

The Wild Sugarcane and Sorghum Kinomes: Insights Into Expansion, Diversification, and Expression Patterns.

The protein kinase (PK) superfamily is one of the largest superfamilies in plants and the core regulator of cellular signaling. Despite this substantial importance, the kinomes of sugarcane and sorghum have not been profiled. Here, we identified and profiled the complete kinomes of the polyploid (Ssp) and (Sbi), a close diploid relative.

Structural and Biochemical Aspects Related to Resistance and Susceptibility of Rubber Tree Clones to Anthracnose.

The aim of the present study was to evaluate structural and biochemical aspects related to the interaction of resistant (RRIM 937, IAC 502 and 507) and susceptible (RRIM 600) rubber tree clones with . For such analysis, ultrathin sections of the leaf limb were embedded in historesin and differently stained to verify structural alterations and presence of starch grains, arginine, lipids, tannins and lignins. The total proteins and activity of the enzymes peroxidase and (PAL) were quantified.

Machine learning approaches reveal genomic regions associated with sugarcane brown rust resistance.

Sugarcane is an economically important crop, but its genomic complexity has hindered advances in molecular approaches for genetic breeding. New cultivars are released based on the identification of interesting traits, and for sugarcane, brown rust resistance is a desirable characteristic due to the large economic impact of the disease. Although marker-assisted selection for rust resistance has been successful, the genes involved are still unknown, and the associated regions vary among cultivars, thus restricting methodological generalization.

Dynamics and resilience of soil mycobiome under multiple organic and inorganic pulse disturbances.

Disturbances in soil can cause short-term soil changes, consequently changes in microbial community what may result in long-lasting ecological effects. Here, we evaluate how multiple pulse disturbances effect the dynamics and resilience of fungal community, and the co-occurrence of fungal and bacterial communities in a 389 days field experiment. We used soil under sugarcane cultivation as soil ecosystem model, and organic residue (vinasse - by-product of sugarcane ethanol production) combined or not with inorganic (organic residue applied 30 days before or together with mineral N fertilizer) amendments as disturbances.

Sorghum Growth Promotion by and : Putative Mechanisms Revealed by Genomics and Metagenomics.

Bacteria from the genera and can promote the growth of , but the underlying mechanisms are not yet known. In a pot experiment, sorghum plants grown on sterilized substrate were inoculated with strain IAC/BECa 135 and strain IAC/BECa 152 under phosphate-deficient conditions. These strains significantly increased cultivar SRN-39 root and shoot biomass.