[The function of helper plasmids from HEP-Flury strain rabies virus on encapsidating the full-length genome of CTN strain].

Objective: To identify the helper plasmids from HEP-Flury strain rabies virus that could encapsidate the full-length genome of CTN strain.

Methods: Four overlapped fragments covering the full-length genome of rabies virus CTN strain were cloned into expression vector. A recombinant full-length genome plasmid (pCTN-GFP) contained the full-length genome of the CTN strain expect for psi gene which was replaced by GFP gene was then constructed using restriction enzyme cleavage and ligation in vitro.