44 results match your criteria: "Agricultural University of China[Affiliation]"
Cell Reprogram
June 2014
1 College of Life Science, Northeast Agricultural University of China, Harbin, 150030, China .
Induced pluripotent stem cells (iPSCs) and nuclear transfer (NT) are two of the primary routes to reprogram differentiated cells back to the pluripotent state. However, it is still unknown whether there is any correlation between the reprogramming efficiency of iPSCs and NT if the same donor cells are employed. In this study, six porcine embryonic fibroblast (PEF) lines from Landrace (L1, L6, L9) or Congjiang local pigs (C4, C5, C6) were used for iPSC induction and NT.
View Article and Find Full Text PDFCell Stem Cell
March 2014
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; Key Laboratory of Translational Stem Cell Research, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China. Electronic address:
The rat is an important animal model in biomedical research, but practical limitations to genetic manipulation have restricted the application of genetic analysis. Here we report the derivation of rat androgenetic haploid embryonic stem cells (RahESCs) as a tool to facilitate such studies. Our approach is based on removal of the maternal pronucleus from zygotes to generate androgenetic embryos followed by derivation of ESCs.
View Article and Find Full Text PDFGenomics Proteomics Bioinformatics
October 2013
College of Life Science, Northeast Agricultural University of China, Harbin 150030, China.
The aim of this study was to optimize electrofusion conditions for generating porcine tetraploid (4n) embryos and produce tetraploid/diploid (4n/2n) chimeric embryos. Different electric field intensities were tested and 2 direct current (DC) pulses of 0.9 kV/cm for 30 μs was selected as the optimum condition for electrofusion of 2-cell embryos to produce 4n embryos.
View Article and Find Full Text PDFGenomics Proteomics Bioinformatics
October 2013
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; College of Life Science, Northeast Agricultural University of China, Harbin 150030, China.
Human induced pluripotent stem cells (iPSCs) hold great promise for regenerative medicine. Generating iPSCs from immunologically immature newborn umbilical cord blood mononuclear cells (UCBMCs) is of great significance. Here we report generation of human iPSCs with great efficiency from UCBMCs using a dox-inducible lentiviral system carrying four Yamanaka factors.
View Article and Find Full Text PDFJ Biol Chem
September 2013
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; Key Laboratory of Translational Stem Cell Research, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China. Electronic address:
The rat is an important animal model for human disease research. Using inhibitors of glycogen synthase kinase 3 and MAPK signaling pathways, rat embryonic stem cells and rat induced pluripotent stem cells (riPSCs) have been derived. However, unlike rat embryonic stem cells, germ line competent riPSCs have only been derived from Wistar rats at low efficiency.
View Article and Find Full Text PDFActa Pharmacol Sin
June 2013
College of Life Science, Northeast Agricultural University of China, Harbin 150030, China.
Stem cells can both self-renew and differentiate into various cell types under certain conditions, which makes them a good model for development and disease studies. Recently, chemical approaches have been widely applied in stem cell biology by promoting stem cell self-renewal, proliferation, differentiation and somatic cell reprogramming using specific small molecules. Conversely, stem cells and their derivatives also provide an efficient and robust platform for small molecule and drug screening.
View Article and Find Full Text PDFStem Cell Rev Rep
October 2013
College of Life Science, Northeast Agricultural University of China, Harbin, 150030, People's Republic China.
Pigs are valuable animal models in pre-clinical research due to their anatomical and similarity to human-beings. Little is known about porcine embryonic development and porcine pluripotent stem cells. Recently, porcine-induced pluripotent stem cells (piPSCs) have been generated with Oct4 (Pou5f1), Sox2, Klf4 and c-Myc (termed OSKM, 4 F).
View Article and Find Full Text PDFPseudoperonospora cubensis is a foliar pathogen of cucurbits. In cucumber, it produces chlorotic, angular lesions with dark sporangia on their under-surface. The distribution of pathotypes and mating types of P.
View Article and Find Full Text PDFJ Genet Genomics
December 2012
College of Life Science, Northeast Agricultural University of China, Harbin 150030, China.
Rat is a valuable model for pharmacological and physiological studies. Germline-competent rat embryonic stem (rES) cell lines have been successfully established and the molecular networks maintaining the self-renewing, undifferentiated state of rES cells have also been well uncovered. However, little is known about the differentiation strategies and the underlying mechanisms of how these authentic rat pluripotent stem cells give rise to specific cell types.
View Article and Find Full Text PDFPLoS One
December 2012
College of Life Science, Northeast Agricultural University of China, Harbin, China.
The arrest of meiotic prophase in mammalian oocytes within fully grown follicles is dependent on cyclic adenosine monophosphate (cAMP) regulation. A large part of cAMP is produced by the Gs-linked G-protein-coupled receptor (GPR) pathway. In the present study, we examined whether GPR3 is involved in the maintenance of meiotic arrest in porcine oocytes.
View Article and Find Full Text PDFGenet Mol Res
February 2012
College of Life Science, Northeast Agricultural University of China, Harbin, China.
Sequences proximal to transgene integration sites are able to regulate transgene expression, resulting in complex position effect variegation. Position effect variegation can cause differences in epigenetic modifications, such as DNA methylation and histone acetylation. However, it is not known which factor, position effect or epigenetic modification, plays a more important role in the regulation of transgene expression.
View Article and Find Full Text PDFYi Chuan
July 2011
College of Life Science, Northeast Agricultural University of China, Harbin 150030, China.
Uncompleted epigenetic reprogramming is attributed to the low efficiency of producing transgenic cloned animals. Histone modification associated with epigenetics can directly influence the embryo development and transgene expression. Trichostatin A (TSA), as an inhibitor of histone deacetylase, can change the status of histone acetylation, improve somatic cell reprogramming, and enhance cloning efficiency.
View Article and Find Full Text PDFCell Mol Biol (Noisy-le-grand)
June 2011
Northeast Agricultural University of China, College of Life Science, Harbin, China.
Transgenic technology has been used for years to study gene function, produce important proteins, and generate models for the study of human diseases. However, the efficiency of producing transgenic animal lines that retain normal function is extremely low. The low efficiency can be mainly attributed to the integrated transgene.
View Article and Find Full Text PDFPLoS One
October 2011
College of Life Science, Northeast Agricultural University of China, Harbin, China.
The low success rate of somatic cell nuclear transfer (SCNT) in mammalian cloning is largely due to imprinting problems. However, little is known about the mechanisms of reprogramming imprinted genes during SCNT. Parental origin-specific DNA methylation regulates the monoallelic expression of imprinted genes.
View Article and Find Full Text PDFYi Chuan
May 2011
College of Life Science, Northeast Agricultural University of China, Harbin 150030, China.
Transgenic technology is one of the most hotspots in biology. In the past decade, the progress in animal cloning has provided an alternative method to improve transgenic efficiency. Many kinds of transgenic animals have been successfully produced via the combination of transfection and nuclear transfer.
View Article and Find Full Text PDFMol Cell Biochem
September 2011
College of Life Science, Northeast Agricultural University of China, No. 59 Mucai Street, Harbin, Heilongjiang 150030, China.
Transgenic technology has greatly facilitated our understanding of gene function, producing pharmaceutical proteins, and generating models for the study of human diseases. However, epigenetic silencing is still the most major limitation. In this study, we employed DNA methyltransferase inhibitor 5-Aza-2'-deoxycytidine (5-Aza-dC) and histone deacetylase inhibitor Trichostatin A (TSA) to study the reactivation of silenced green fluorescent protein (GFP) transgene driven by the cytomegalovirus (CMV) promoter in three fibroblast cell lines from transgenic pigs (tPFs).
View Article and Find Full Text PDFPLoS One
August 2009
College of life science, Northeast Agricultural University of China, Harbin, People's Republic of China.
Transgenic animals have been used for years to study gene function, produce important proteins, and generate models for the study of human diseases. However, inheritance and expression instability of the transgene in transgenic animals is a major limitation. Copy number and promoter methylation are known to regulate gene expression, but no report has systematically examined their effect on transgene expression.
View Article and Find Full Text PDFGuang Pu Xue Yu Guang Pu Fen Xi
May 2009
The Institute of Grassland Research of Animal Scientific College of Agricultural University of China, Beijing, China.
Stratospheric ozone depletion occurs mainly over polar regions during the spring when the solar ultraviolet B-band (280-315 nm, UV-B) radiation is most intense in a year, but over the Qing Tibetan Plateau region, the intensity is highest from June to September when the amount of UV-B radiation reaching the regions is more than that in the adjacent areas lying in the same latitude by 10%. From June to September is just the time of plant's germination, development, and reproduction in the alpine region. The enhanced UV-B radiation may alter the rate of senescence in the forage plant, oats (Avena sativa), which plays the vital role in developing indigenous herdsman's animal husbandry industry.
View Article and Find Full Text PDFPlant Physiol Biochem
October 2008
Key Laboratory of Tea Biochemistry and Biotechnology, Ministry of Education and Ministry of Agriculture, Anhui Agricultural University of China, Hefei, Anhui 230036, China.
ATP sulfurylase, the first enzyme in the sulfate assimilation pathway of plants, catalyzes the formation of adenosine phosphosulfate from ATP and sulfate. Here we report the cloning of two cDNAs encoding ATP sulfurylase (APS1 and APS2) from Camellia sinensis. They were isolated by RT-PCR and RACE-PCR reactions.
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