Inhibition of triglyceride metabolism-associated enhancers alters lipid deposition during adipocyte differentiation.

Triglyceride (TG) metabolism is a complex and highly coordinated biological process regulated by a series of genes, and its dysregulation can lead to the occurrence of disorders in lipid metabolism. However, the transcriptional regulatory mechanisms of crucial genes in TG metabolism mediated by enhancer-promoter interactions remain elusive. Here, we identified candidate enhancers regulating the Agpat2, Dgat1, Dgat2, Pnpla2, and Lipe genes in 3T3-L1 adipocytes by integrating epigenomic data (H3K27ac, H3K4me1, and DHS-seq) with chromatin three-dimensional interaction data.

The SlWRKY42-SlMYC2 module synergistically enhances tomato saline-alkali tolerance by activating the jasmonic acid signaling and spermidine biosynthesis pathway.

Tomato (Solanum lycopersicum) is an important crop but frequently experiences saline-alkali stress. Our previous studies have shown that exogenous spermidine (Spd) could significantly enhance the saline-alkali resistance of tomato seedlings, in which a high concentration of Spd and jasmonic acid (JA) exerted important roles. However, the mechanism of Spd and JA accumulation remains unclear.

Synergistic antimicrobial activity of essential oils mixture of Moringa oleifera, Cinnamomum verum and Nigella sativa against Staphylococcus aureus using L-optimal mixture design.

The urgent need to address the growing problem of antimicrobial resistance in multidrug-resistant bacteria requires the development of pioneering approaches to treatment. The present study aims to evaluate the antimicrobial potential of the essential oils (EOs) of Moringa oleifera (moringa), Cinnamomum verum (cinnamon), and Nigella sativa (black seed) and the synergistic effect of the mixture of these oils against Staphylococcus aureus MCC 1351. Statistical modeling revealed cinnamon oil had the highest individual antimicrobial potency, followed by black seed oil.

Genetic analysis of growth traits in Salem Black goats under farm conditions.

In this study, data on pedigree, production traits for 19 years (2002-2022) of Salem Black goat from the Mecheri Sheep Research Station, Tamil Nadu, India was used. Various growth traits viz., birth weight (BW), weaning weight (WW), 6-month weight (6W), 9-month weight (9W), 12-month weight (YW), pre- and post-weaning average daily gain (ADG) (3-6, 6-9, 9-12 and 3-12 months) were analyzed.

Hydrogeochemical characterization of shallow and deep groundwater for drinking and irrigation water quality index of Kathmandu Valley, Nepal.

A comprehensive hydrogeochemical analysis of 156 groundwater samples (106 shallow and 50 deep) was conducted in the Kathmandu Valley, Nepal. This study addresses a significant research gap by focusing on the hydro-geochemical composition and contamination of groundwater in the Kathmandu Valley, an area with limited detailed assessments. The novelty of this work lies in its comprehensive analysis of both shallow and deep groundwater, particularly concerning the high concentration of contaminants like arsenic, microbial pathogens, and ammonium, which are critical for public health.

Performance of weighted genomic BLUP and Bayesian methods for Hanwoo carcass traits.

To improve the quality and yield of the Korean beef industry, selection criteria often focus on estimated breeding values for carcass weight (CWT), eye muscle area (EMA), backfat thickness (BF), and marbling score (MS). This study estimated genetic parameters and assessed the accuracy of genomic estimated breeding values (GEBVs) using SNP weighting methods. We compared the accuracy of these methods with the genomic best linear unbiased prediction (GBLUP) and various Bayesian approaches (BayesA, BayesB, BayesC, and BayesCPi) for the specified traits.

The effect of single versus group culture on cumulus-oocyte complexes from early antral follicles.

Purpose: This study aimed to evaluate the effectiveness of single versus group culture strategies for cumulus-oocyte complexes (COCs) derived from early antral follicles (EAFs), with the goal of optimizing culture conditions to increase oocyte availability for assisted reproductive technologies.

Methods: COCs isolated from EAFs (350-450 µm) from sheep ovaries were cultured in TCM199 medium supplemented with 0.15 µg/mL Zn as zinc sulfate, 10 IU/mL FSH, 10 ng/mL estradiol, 50 ng/mL testosterone, 50 ng/mL progesterone, and 5 µM Cilostamide.

Exploiting the efficient Exo:Cas12i3-5M fusions for robust single and multiplex gene editing in rice.

The development of a single and multiplex gene editing system is highly desirable for either functional genomics or pyramiding beneficial alleles in crop improvement. CRISPR/Cas12i3, which belongs to the Class II Type V-I Cas system, has attracted extensive attention recently due to its smaller protein size and less restricted canonical "TTN" protospacer adjacent motif (PAM). However, due to its relatively lower editing efficiency, Cas12i3-mediated multiplex gene editing has not yet been documented in plants.

Bioinformatics and immunoinformatics approaches in the design of a multi-epitope vaccine targeting CTLA-4 for melanoma treatment.

Melanoma, a highly aggressive skin cancer, remains a significant cause of mortality despite advancements in therapeutic strategies. There is an urgent demand for developing vaccines that can elicit strong and comprehensive immune responses against this malignancy. Achieving this goal is crucial to enhance the efficacy of immunological defense mechanisms in combating this disease.

The content and associated health risk assessment of toxic elements, micro-, and macrominerals in common carp, Wels catfish, and silver carp from the Danube River in Serbia.

Three fish species (common carp, Wels catfish, and silver carp) were collected from three locations along the Danube River in Serbia, and fish meat was analyzed for the content of toxic elements, micro- and macrominerals. Silver carp had the highest lead (Pb), arsenic (As), and cadmium (Cd) content, while Wels catfish had the highest level of mercury (Hg). Moreover, metal pollution index (MPI) ranged from 0.

Morphological identification and phylogenetic analysis of Eimeria coypi and Eimeria fluviatilis (Apicomplexa: Eimeriidae) isolated from nutrias (Myocastor coypus [Rodentia]) in Japan.

The nutria (Myocastor coypus) is a semiaquatic rodent that originally inhabited South America. However, the animals have spread to different continents as alien species, and their numbers are quickly increasing, especially in North America, Europe, and Eastern Asia including Japan. Although nutrias have been suggested to serve as reservoirs for pathogens, including parasites, there have been few reports on this subject.

Genetics of sorghum: grain quality, molecular aspects, and drought responses.

Sorghum kernel composition is a crucial characteristic that determines its functional qualities. The total protein content of sorghum grain increases under drought stress, but starch, protein digestibility, and micronutrient contents decrease. Sorghum (Sorghum bicolor L.

Simultaneous site-directed mutagenesis for soybean ß-amyrin synthase genes via DNA-free CRISPR/Cas9 system using a single gRNA.

We generated soybean mutants related to two ß-amyrin synthase genes using DNA-free site-directed mutagenesis system. Our results suggested that one of the genes is predominant in the soyasaponin biosynthesis. Soyasaponins, which are triterpenoid saponins contained in soybean [Glycine max (L.

Molecular docking and differential rbcl gene expression reveal variation in glyphosate herbicide tolerance of sugarcane varieties.

Background: Glyphosate is an extensively employed herbicide in agriculture, specifically for sugarcane cultivation. The situation is different with the extensive physiological and genetic effects exerted by this herbicide on a range of plant species, including sugarcane, whose model basis is still poorly characterized, although its primary mode of action, which acts on the EPSPS enzyme in the shikimic acid pathway, is completely elucidated. The current study was aimed at investigating the stability of glyphosate formulation, molecular interactions of glyphosate formulation with rbcL enzyme associated with chlorophyll metabolism, and its effects on varieties of sugarcane.

Effect of transgene on salt tolerance of tobacco.

To explore the effects of salt-tolerance gene accumulation on salt tolerance in transgenic plant, we used four types of plant expression vector (N27, N28, N29, and N30) carrying mtlD, mtlD + gutD, mtlD + gutD + BADH, mtlD + gutD + BADH + sacB genes respectively, to transform tobacco through Agrobacterium-mediated method. Transgenic lines were identified through polymerase chain reaction (PCR) detection. Transgenic lines and non-transgenic plant (CK) were subjected to 6‰ sodium chloride solution stress; then, fluorescence quantitative PCR (FQ-PCR) and salt tolerance indexes were used to assess characteristics.

ABC-type salt tolerance transporter genes are abundant and mutually shared among the microorganisms of the hypersaline Sambhar Lake.

To fish-out novel salt-tolerance genes, metagenomic DNA of moderately saline sediments of India's largest hypersaline Sambhar Lake was cloned in fosmid. Two functionally-picked clones helped the Escherichia coli host to tolerate 0.6 M NaCl.

Effects of variable mating opportunity, delay, and male mating experience on the lifespan, female reproductive traits, and offspring traits of Phytoseiulus persimilis (Acari: Phytoseiidae).

The balance between mating benefits and costs shapes reproductive strategies and life history traits across animal species. For biological control programs, understanding how mating rates influence life history traits is essential for optimising population management and enhancing predator efficacy. This study investigates the impact of mating opportunity availability, delayed mating, and male mating history (copulation frequency) on the lifespan (both sexes), female reproductive traits (duration of oviposition and of pre- and post-oviposition periods, and lifetime oviposition), and offspring quality (egg size and offspring survival) of the predatory mite Phytoseiulus persimilis Athias-Henriot (Acari: Phytoseiidae), an important biological control agent against spider mites.

Development and utilization of a multiplex PCR assay for detecting three feline enteroviruses.

Background: Feline diarrhea is a common digestive tract disease in clinical practice, with watery feces as the main clinical manifestation. There are numerous pathogenic factors causing feline diarrhea, among which viral infections are prevalent, and feline panleukopenia virus (FPV) is the most common pathogen. In recent years, a variety of novel viruses have been detected in the intestines of cats with diarrhea.

Spatial and temporal variability of soil moisture active and passive (SMAP) droughts and their impacts on vegetation in the Central Highlands of Vietnam.

Drought is a reoccurring natural phenomenon that presents significant challenges to agricultural production, ecosystem stability, and water resource management. The Central Highlands of Vietnam, a major region of industrial crops and vegetation ecosystems, has become increasingly vulnerable to drought impacts. Despite this vulnerability, limited research has explored the specific characteristics of drought and its seasonal effects on vegetation ecosystems in the region.

The status of pyrethroid resistance mutation frequencies in Varroa destructor populations in the most important beekeeping areas of Türkiye.

The Varroa destructor (hereafter referred to as Varroa) is a major pest of honeybees that is generally controlled using pyrethroid-based acaricides. However, resistance to these insecticides has become a growing problem, driven by the acquisition of knockdown resistance (kdr) mutations in the mite's voltage-gated sodium channel (vgsc) gene. Resistance mutations in the vgsc gene, such as the L925V mutation, can confer resistance to pyrethroids like flumethrin and tau-fluvalinate.

Electrochemical capacitance-based aptasensor for HER2 detection.

The overexpression of Human Epidermal Growth Factor Receptor 2 (HER2) protein is specifically related to tumor cell proliferation in breast cancers. Its presence in biological serum samples indicates presence or progression of cancer, becoming a promise biomarker. However, their detection needs a simple and high accuracy platform.

In silico screening and immunogenic features of putative tick cement protein PA107 from Ixodes ricinus tick.

Tick salivary proteins are crucial for efficient and successful tick feeding. Most of them are still uncharacterized, especially those involved in the formation of tick cement. Tick salivary protein PA107 is a putative cement protein, which is transcribed in salivary glands during the initial phase of tick feeding.

Cytokinin negatively regulates tomato fruit ripening by influencing the ethylene pathway.

Reducing endogenous CK levels accelerates fruit ripening in tomato by regulating ethylene biosynthesis and signalling pathway. Tomato is a typical climacteric fruit and is recognized as one of the most important horticultural crops globally. The ripening of tomato fruits is a complex process, highly regulated by phytohormones.