[Some influence factors in determination of serum enzyme in hepatitis patients].

Objective: To study some factors (temperature, time, anticoagulate, reagent, apparatus) which influence the detection of serum enzyme.

Methods: Serums obtained from same patient are determined in different conditions. Compare the statistical difference among each group.

[Analysis of resistant genes of beta-lactam antibiotics from Pseudomonas aeruginosa in pediatric patients].

Objective: To analyze the antibiotic resistance of the Pseudomonas aeruginosa (PA) isolated from pediatric patients and the resistant genes of beta-lactam antibiotics thereof.

Methods: 146 PA strains were isolated from pediatric patients. Agar dilution method recommended by the Clinical and Laboratory Standards Institute was used to examine the minimum inhibitory concentrations (MICs) of 12 antimicrobial agents, including the penicillins, third and fourth genet ration cephalosporins, carbapenemase, Aztreonam, beta-lactamase inhibitors, quinolones, and aminoglycosides.

[Study on the distribution and correlation of trace elements in whole blood of children in Beijing].

Objective: To study the distribution and correlation of trace elements in peripheral blood of children in Beijing so as to offer scientific evidence for the supplementation of trace elements and to prevent lead intoxication.

Methods: Contents of trace elements in whole blood of 13 929 children in Beijing region were detected by Atomic Absorption Spectrometer. According to developmental condition, children were divided into young infancy group, infancy group, toddler's age group, preschool age group, school age group and adolescence group.

[Application of molecular method in identification of bacterial endophthalmitis].

Objective: To search for a non-culture method to improve the efficiency of etiological diagnosis of infective endophthalmitis and other infectious diseases.

Methods: Fifty clinical specimens of infective endophthalmitis were collected. DNA of bacterium was extracted directly from the aqueous fluid or vitreous fluid and then amplified with universal primers.

[Total lumbar disc replacement with SB Charité III prosthesis: Chinese experience with more than two years follow-up].

Objective: To evaluate the clinical and radiographic results of total lumbar disc replacement with SB Charité III prosthesis.

Methods: From Dec 1999 to Dec 2006, total lumbar disc replacement with SB Charité III prosthesis was performed in 65 patients affected with degenerative lumbar disc disorders. Among these patients, 48 (52 prosthesis) were followed up for more than two years (from 2.

[Randomized controlled multi-central study on acupuncture at Tanzhong (CV 17) for treatment of postpartum hypolactation].

Objective: To study on clinical role of acupuncture at Tanzhong (CV 17) for treatment of postpartum hypolactation and to provide clinical basis for indications of acupoints.

Methods: This was a single blind randomized controlled multi-center study by Beijing obstetrical and gynecological hospital, Beijing Mother and Child health institute and Haidian Mother and Child health institute, 276 cases of postpartum hypolactation were randomly divided into an acupuncture group and a Chinese drug group. The acupuncture group were treated with acupuncture at Tanzhong (CV 17) and the Chinese drug group with traditional drug Tongre Decoction.

Streptococcus pneumoniae diseases in Chinese children: past, present and future.

This review describes major contributions to our understanding of pneumococcal diseases among children in hinterland of China. The data demonstrated that Streptococcus pneumoniae (S. pneumoniae) is an important pathogen of pyogenic meningitis, pneumonia, and other infectious diseases in children.

Potential of stem cell based therapy and tissue engineering in the regeneration of the central nervous system.

The insufficiency of self-repair and regeneration of the central nervous system (CNS) leads to difficulty of rehabilitation of the injured brain. In the past few decades, the significant progress in cell therapy and tissue engineering has contributed to the functional recovery of the CNS to a great extent. The present review focuses on the potential role of stem cell based therapy and tissue engineering in the regeneration of the CNS.

[Clinical features of 17 cases of X-linked agammaglobulinemia].

Objective: To investigate clinical features of X-linked agammaglobulinemia (XLA) in children.

Methods: The medical records of 17 children with XLA between January 2001 and April 2007 were reviewed.

Results: The age at first diagnosis in 88.

Influence of gum chewing on return of gastrointestinal function after gastric abdominal surgery in children.

Purpose: The study was designed to investigate the influence of gum chewing on the return of gastrointestinal function after gastric abdominal surgery in children and the action of neural and humoral hormones in the mechanism of gum chewing.

Material And Methods: Eighteen patients were enrolled in our study. Each patient underwent gastrointestinal surgery and was then randomly assigned to either the gum-chewing or the control group.

[Discussion of correlativity between acupoints and Goldenberg's tender points].

Objective: To study clinically on acupoints by probing the correlativity between Goldenberg's tender points in the patient of fibromyalgia syndrome and acupoints.

Methods: Local hyaluronidase injection and other methods were adopted.

Results: The tender points were increased by more than 50 groups according to the standard of Goldenberg's tender points.

[Changes of blood coagulative and fibrinolytic system and function of pulmonary vascular endothelium after therapy in patients with acute pulmonary thromboembolism].

Objective: To study the changes of blood coagulative and fibrinolytic system and the function of pulmonary vascular endothelium in the course of acute pulmonary thromboembolism (PTE) and after anticoagulant or thrombolytic treatment.

Methods: Twenty patients with acute non-massive PTE, 10 males and 10 females, aged (57 +/- 11) underwent anticoagulant treatment and 17 sex-, and age-matched acute massive PTE patients underwent thrombolytic treatment. The plasma level of D-dimer (D-D), thrombomodulin (TM), protein C (PC), protein S (PS), tissue-type plasminogen activator (t-PA), plasminogen activator inhibitor-1 (PAI-1), and antithrombin-III (AT-III) activity were measured by ELISA before and after normal subjects severed as control group were included in the study.

[Energy metabolism in patients with severe chronic viral hepatitis].

Objective: To evaluate the pattern of energy metabolism of patients with chronic viral severe hepatitis.

Methods: Resting energy expenditure (REE) in 55 patients with chronic viral severe was measured with open-circuit indirect calorimetry. Their normal REE was predicted by Harris-Benedict equation (HBE).

[Length and volume of intravesical prostatic protrusion closely correlated with bladder outflow obstruction in BPH patients].

Objective: To analyze the correlation of the length and volume of intravesical prostatic protrusion (IPP) with bladder outflow obstruction (BOO) in patients with BPH and to find a simple method for the diagnosis of the disease.

Method: The length and volume IPP were measured by transrectal ultrasound for 87 patients with BPH, the diagnosis of BOO was made by urodynamic tests and the correlation of the length and volume of IPP with BOO was analyzed, and reanalyzed 3 months after oral medication of a-blocker. The length and volume of IPP were measured again during the operation in 54 of the cases to confirm the ultrasound findings.

[Experimental study on effect of Bailing Cpsule on dendritic cells in mice].

Objective: Comparing with the classic immuno-supressors, to probe in the in vitro effect of Cordyceps Sinensis (CS) on the differentiation, maturation and function of dentritic cells (DCs), and further to explore its mechanism.

Methods: Mice myeloid DCs were cultured respectively with extraction of Bailing Capsule (CSE), a Chinese medical preparation made of CS, Rapamycin and Tacrolimus, and the effect of various drugs on phenotype of DCs was analyzed with flow cytometer. Then, using as the stimulator, the DCs cultured with different drugs were mixed and cultured with heterogenous lymphocytes for observing the stimulating capacity of DCs on cell proliferation.

[Changes and significance of peripheral blood fibrinogen level in patients with severe acute respiratory syndrome].

Objective: To investigate variations and significance of plasma fibrinogen in patients with severe acute respiratory syndrome (SARS).

Methods: Totally 148 patients with SARS were divided into the following groups: initial stage group (44 cases) and after the initial stage group (104 cases), common type group (87 cases) and severe type group (61 cases), unilobar lung involvement group (49 cases), bilobar lung involvement group (53 cases) and diffuse lung involvement group (46 cases). The values of plasma fibrinogen of the 148 SARS patients were analyzed and compared among the different groups.

[Diffuse panbronchiolitis in a child: case report and literature review].

Objective: Diffuse panbronchiolitis (DPB) is a chronic progressive disease of the lower respiratory tract, which is prevalent in Asian population. So far, many DPB cases have been found in adults in China. To our knowledge, no pediatric DPB case has ever been reported in China.

[Clinical study of the impact of a history of chronic diseases on the mortality of patients with multiple organ dysfunction syndrome in the elderly].

Objective: To analyze the clinical feature of multiple organ dysfunction syndrome in the elderly (MODSE) and study the impact of a history of chronic diseases on the mortality of patients with MODSE.

Methods: Altogether 331 cases with MODSE were prospectively analyzed in 4 tertiary-level teaching hospitals in Beijing and Tianjin cities from March 2002 to January 2005.

Results: In our investigation,the primary etiology of MODSE was severe infection (27.

[Morphologic observation and pathogenesis investigation of regenerated sinusoidal endothelial cells in remodelling rat hepatic necrotic tissue].

Objective: To investigate the morphological changes and regeneration mechanism of sinusoidal endothelial cell.

Methods: Sixty male Wistar rats (bought from SLC company limited of Japan) were divided into three groups. Fifty of them belonged to experiment group, five rats belonged to untreated group, and the rest five ones belonged to normal saline treated group.

Are impaired endothelial progenitor cells involved in the processes of late in-stent thrombosis and re-endothelialization of drug-eluting stents?

Drug-eluting stent (DES) now is the default selection for most of the interventional cardiologists. However, its benefits compromised by the stent-related thrombosis events. Given the catastrophic consequences, it is important to investigate possible mechanisms of stent thrombosis.

[Relationship between metabolic syndrome and 10-year risk for incident type 2 diabetes mellitus].

Objective: To analyze the relationship of metabolic syndrome (MS) and its components at baseline to the 10-year incidence of diabetes mellitus.

Methods: An investigation on cardiovascular disease (CVD) risk factors, including questionnaire survey, physical examination, and laboratory examination, was carried out in the populations of 4154 subjects, aged 35 approximately 64, in 2 communities (Peking University and Capital Iron and Steel Company area) collected by cluster sampling in the year 1992, with a response rate of 82.0%, so as to obtain baseline data.

Antimicrobial resistance and serotypes of nasopharyngeal strains of Streptococcus pneumoniae in Chinese children with acute respiratory infections.

This prospective, multicentre, nasal carriage study in Chinese children with upper respiratory infection was carried out over the period from 2000 to 2002. Overall, the prevalence of pneumococcal carriage was 24.9%.