An experimental hyperlipidemia model with periodontitis in mice.

Purpose: In many animal models and clinical trials, the relationship between periodontitis and hyperlipidemia is bidirectional and interlinked. In this study, an experimental hyperlipidemia model with periodontitis in mice is introduced.

Methods: C57BL/6J mice were assigned into group A and B and Apolipoprotein E-deficient (ApoE) mice into group C.

Spatial and temporal expression of SP-B and TGF-β1 in hyperoxia-induced neonatal rat lung injury.

Objective: Bronchopulmonary dysplasia (BPD) is a severe complication of extreme prematurity that can be caused by hyperoxia inhalation. SP-B and TGF-β have been reported to be implicated in the development of lung. This study aimed to reveal the spatial and temporal expression patterns of these two factors in an animal model of BPD.

Angiopoietin-1 alleviates LPS-induced inflammatory injury by up-regulation of miR-126 in pancreas cell line HPDE6-C7.

Background: Acute pancreatitis is an inflammatory disorder of the pancreas, leading to multiple organ dysfunction syndrome in severe cases. Angiopoietin-1 (Ang-1), a Tie-2 receptor agonist, and microRNA-126 (miR-126) have been reported to be involved in angiogenesis and anti-inflammatory functions. In the present study, we explored the effects of Ang-1 and miR-126 on lipopolysaccharide (LPS)-induced inflammatory injury in pancreatic cells, HPDE6-C7.

[Comparative study of functional neck dissection and radical neck dissection for early oral squamous cell carcinoma].

Purpose: To evaluate the clinical effects of functional neck dissection(FND) and radical neck dissection(RND) on early oral squamous cell carcinoma (OSCC).

Methods: A total of 63 OSCC patients were divides into 2 groups. Patients in FND group (n=30) underwent functional neck dissection with preservation of sternocleidomastoid muscle, accessory nerve, internal jugular vein and great auricular nerve, while patients in RND group (n=33) underwent radical neck dissection.

Perceptions, attitudes, and current practices regards delirium in China: A survey of 917 critical care nurses and physicians in China.

The purpose of this study is to assess the knowledge, attitudes, and managements regarding delirium of intensive care nurses and physicans, and to assess the perceived barriers related to intensive care unit (ICU) delirium monitoring in China. A descriptive survey was distributed to 1156 critical care nurses and physicians from 74 tertiary and secondary hospitals across Shandong province, China. The overall response rate was 86.

Serum miR-34a is a potential diagnostic and prognostic marker for osteosarcoma.

Background And Aims: MicroRNA-34a (miR-34a) has been shown to be a potential diagnostic and prognostic marker for several cancers. In addition, miR-34a has been reported to suppress osteosarcoma cell proliferation in vitro. However, the clinical value of miR-34a is still unknown.

MicroRNA-139-5p alleviates UVB-induced injuries by regulating TLR4 in si-IL-6-treated keratinocyte cells.

Lupus erythematosus (LE) is an autoimmune disease that is often exacerbated by ultraviolet B (UVB) irradiation. MicroRNAs (miRNAs) play vital roles in response to UVB damage to keratinocyte cells. Herein, our study aimed to explore the functions of miR-139-5p in UVB-induced injuries in keratinocyte cells.

Altered patterns of gene expression distinguishing unruptured abdominal aortic aneurysms from ruptured ones: comprehensive analysis of inflammatory factors.

The purpose of this study was to profile altered patterns of gene expression that characterize abdominal aortic aneurysm and to compare these patterns between different conditions, unruptured (URA) and ruptured (RA). Full-thickness aortic wall tissues were obtained from patients during surgical repair of abdominal aortic aneurysm, including unruptured (n=29) and ruptured (n=11). RNA, protein and blood samples were prepared for each specimen, and differential levels of gene expression between unruptured and ruptured abdominal aortic tissues were assessed by immunohistochemistry, RT-qPCR and ELISA assays.

MiR-148a suppresses invasion and induces apoptosis of breast cancer cells by regulating USP4 and BIM expression.

MicroRNAs (miRs), acting as tumor suppressor or oncogenes genes, play a critical role in controlling tumor invasion, metastasis and survival via regulating a variety of targets. MiR-148a has been observed low expressed in several types of human cancers, and overexpression of miR-148a inhibits tumorigenesis. However, the molecular mechanisms of miR-148a-mediated these effects are largely elusive.

Neuron-derived netrin-1 and netrin-4 proteins are additional effective targets in diabetic retinopathy beyond VEGF.

Vascular endothelial growth factor (VEGF) is the typical representative factor of diabetic retinopathy (DR) and is considered to be a key inducer of retinal vascular permeability in DR. Anti-VEGF has been widely used in clinical treatment but every patient is effective, therefore, it is necessary to find other effective factors that participate in the pathology of DR. We provide evidence from both human and animal experiments for the considerable roles of classical neuronal guidance factors, netrin-1 and netrin-4, in indicating and amending the pathology of DR.

The role of ATP8A1 in non-small cell lung cancer.

The study objective was to investigate the expression of ATP8A1 in non-small cell lung cancer (NSCLC) and to discover the role of ATP8A1 in the carcinogenesis of NSCLC. We collected 25 cases of tumor tissues and the adjacent normal tissues from surgeries of NSCLC patients in our hospital, among which 15 cases were found with lymph node metastasis while the other 10 were not. Immunohistochemical staining was performed to compare the expression level of TAP8A1 protein in NSCLC tissues with or without lymph node metastasis and the adjacent normal tissues.

Ultrastructural pathological alterations in ocular muscles in a cat model with exotropia.

To study the ultrastructural alterations of the ocular muscles in a cat model with exotropia. A total of 18 cats (4-6 weeks old) were included in the study and randomly divided into experimental group (n=12) and sham group (n=6). The experimental group underwent surgery to generate the exotropia model.

Lipid and hyperglycemia factors in first-ever penetrating artery infarction, a comparison between different subtypes.

Background: The pathogenesis and progression of branch atheromatous disease (BAD), which differs from lipohyalinotic degeneration (LD), remains controversial. Few studies have investigated the lipid indices and glycometabolism status factors for BAD in first-ever penetrating artery infarction (PAI).

Methods: We retrospectively examined acute stroke patients with PAI admitted within 3 days after stroke.

[Expression and significance of human Dachshund homolog 1 in tongue squamous cell carcinoma and tongue atypical hyperplasia].

Purpose: To investigate the expression and role of human Dachshund homolog1(DACH1)in the development and prognosis of tongue squamous cell carcinoma(TSCC).

Methods: The expression of DACH1 was detected immunohistochemistrically in 51 samples of paraffin-embedded TSCC, paired adjacent tissues and 25 samples of atypical hyperplasia tissues of the tongue. Statistical analysis was performed using SPSS 16.

ASPP2 Plays a Dual Role in gp120-Induced Autophagy and Apoptosis of Neuroblastoma Cells.

HIV invasion of the central nervous system (CNS) in the majority of patients infected with HIV-1, leads to dysfunction and injury within the CNS, showing a variety of neurological symptoms which was broadly termed HIV-associated neurocognitive disorder (HAND). But the molecular mechanisms are not completely understood. It has been suggested that apoptosis and autophagic dysfunction in neurons may play an important role in the development of HAND.

Targeting TGF-β1 suppresses survival of and invasion by anaplastic thyroid carcinoma cells.

Background And Aims: Overexpression of transforming growth factor (TGF)-β1 has been implicated in promoting cell survival, migration and invasion in many cancers, including anaplastic thyroid cancer (ATC). In the present study, we studied the effect of suppressing TGF-β1 by RNA silencing on the survival, invasion and metastasis of ATC cells.

Methods: Small interfering RNA (siRNA) constructs targeting TGF-β1 were validated and used to develop clonal derivatives of the ATC cell line, 8505C.

[Multiple myeloma with oral amyloidosis: report of a case and literature review].

Multiple myeloma is the most common type of multifocal plasma cell proliferation in the bone marrow, whereas cases with multiple amyloidosis of oral cavity are rarely documented. Clinically, the disease is easily miss- diagnosed and mistreated due to lack of specificity. This article reported a rare case with multiple myeloma and amyloidosis of tongue, gingiva, buccal mucosa, followed by a review of relevant literature.

[Synovial chondromatosis in the temporomandibular joint: report of 2 cases].

The clinical manifestations, radiographic findings, intraoperative view, histopathologic features of synovial chondromatosis（SC） in the temporomandibular joint (TMJ) were summarized in 2 cases. Preoperative symptoms included preauricular pain（2/2）, swelling（2/2） and limitation of mouth opening (0/2). X-ray findings showed widened joint space.

The Inhibitory Effects of Nesfatin-1 in Ventromedial Hypothalamus on Gastric Function and Its Regulation by Nucleus Accumbens.

The aim of this study was to investigate the effect of nesfatin-1 signaling in the ventromedial hypothalamus (VMH) on gastric functions, as well as the regulation of these effects by nucleus accumbens (NAc) projections to VMH. The expression of c-fos in nesfatinergic VMH neurons induced by gastric distension (GD) was measured using the double fluoro-immunohistochemical staining. The firing rates of neurons were monitored with single-unit extracellular electric discharge recording.

MicroRNA-195 suppresses colorectal cancer cells proliferation via targeting FGF2 and regulating Wnt/β-catenin pathway.

Colorectal cancer (CRC) is a prevalent cancer with high mortality worldwide. This study was aimed to explore the functional effects of microRNA-195 (miR-195) on CRC cells and the underling mechanism involved. quantitative PCR (qPCR) was performed to monitor the expression of miR-195 in CRC tissues and cell lines.

Serum glycoprotein-derived N- and O-linked glycans as cancer biomarkers.

Early detection of cancer is the key to improving survival. Since most clinically used serum cancer biomarkers are either glycoproteins or glycan structures that can be recognized by specific monoclonal antibodies, developing glycan structure-based biomarkers from human serum/plasma glycoproteins through mass spectrometry (MS) analysis are active research field during the past decades. Numerous studies have shown that changes in serum/plasma glycan structures occur during cancer initiation, progression, and treatment.

miR-221 promotes growth and invasion of hepatocellular carcinoma cells by constitutive activation of NFκB.

Background And Objective: microRNAs (miRs) are small noncoding RNAs that modulate a variety of cellular processes by regulating multiple targets, which can promote or inhibit the development of malignant behaviors. Accumulating evidence suggests that microRNA-221 (miR-221) plays important roles in human carcinogenesis. It has recently found that miR-221 was overexpressed in hepatocellular carcinoma (HCC), and overexpression of miR-221 has a bad prognosis in these patients.

Targeting of slug sensitizes anaplastic thyroid carcinoma SW1736 cells to doxorubicin via PUMA upregulation.

Anaplastic thyroid carcinoma (ATC) is one of the most aggressive human cancers and often shows resistance to multimodal therapeutic approaches. It has been shown that the transcriptional repressor Slug inhibits the chemotherapeutic agent-induced apoptosis of cancer cells. We evaluated whether targeting of Slug could augment doxorubicin (DOX)-induced apoptosis of ATC cells.

Propofol inhibits growth and invasion of pancreatic cancer cells through regulation of the miR-21/Slug signaling pathway.

Aim: Propofol, an intravenous anesthetic agent, has been found to inhibit invasion and growth of pancreatic cancer cells . However, the mechanisms underlying these tumor-promoting phenotypes are not known. The microRNA miR-21 has been reported to be overexpressed in pancreatic cancer, and overexpression of miR-21 confers a poor prognosis to patients with pancreatic cancer.