A functional autophagy pathway is essential for BMP9-induced osteogenic differentiation of mesenchymal stem cells (MSCs).

Mesenchymal stem cells (MSCs) are capable of differentiating into bone, cartilage and adipose tissues. We identified BMP9 as the most potent osteoinductive BMP although detailed mechanism underlying BMP9-regulated osteogenesis of MSCs is indeterminate. Emerging evidence indicates that autophagy plays a critical role in regulating bone homeostasis.

Mesenchymal stem cell-derived exosomes: a promising vector in treatment for diabetes and its microvascular complications.

Mesenchymal stem cell-derived exosomes (MSC-exos) are phospholipid bimolecular vesicles containing various materials, and they mediate crosstalk among cells. MSC-exos can maintain glucose homeostasis and delay the progression of diabetes and its microvascular complications through multiple mechanisms, such as by improving β-cell viability and insulin resistance as well as through multiple signal transduction pathways. However, related knowledge has not yet been systematically summarized.

Research progress of pre-hospital emergency during 2000-2020: a bibliometric analysis.

Background: The past decade has witnessed the preliminary development of pre-hospital emergency. We analyzed the scientific output related to pre-hospital emergency in the past two decades, aiming to evaluate the publication status of the literature related to pre-hospital emergency through bibliometrics analysis, and hope to provide enlightenment of trends and hotspots for the development of pre-hospital emergency.

Method: By web of science, all literature on pre-hospital emergency from 2000 to 2 October 2020 was retrieved and screened by two researchers.

The role of an immune signature for prognosis and immunotherapy response in endometrial cancer.

Immunotherapy is a practical and promising treatment for advanced and recurrent endometrial cancer (EC). In this study, we identified an immune-related gene (IRG) signature to predict the overall survival (OS) and response to immune checkpoints inhibitors (ICIs) in patients with EC. The RNA expression profiles of EC were obtained from The Cancer Genome Atlas database and then were filtered for IRGs based on the Immport database.

A CT-based radiomics nomogram for distinguishing between benign and malignant bone tumours.

Background: We sought to evaluate the performance of a computed tomography (CT)-based radiomics nomogram we devised in distinguishing benign from malignant bone tumours.

Methods: Two hundred and six patients with bone tumours were spilt into two groups: a training set (n = 155) and a validation set (n = 51). A feature extraction process based on 3D Slicer software was used to extract the radiomics features from unenhanced CT images, and least absolute shrinkage and selection operator logistic regression was used to calculate the radiomic score to generate a radiomics signature.

[Evaluation on the fracture resistance of dental tissues after guided plate-mediated precision minimally invasive root canal treatment].

Purpose: In order to achieve accurate and minimally invasive root canal treatment and enhance the fracture resistance of tooth tissue after root canal therapy, this study explores digital guided mediated minimally invasive root canal treatment and compares it with conventional root canal treatment to provide a more favorable method for clinical practice.

Methods: Forty freshly extracted first permanent molars were randomly divided into control group and experimental group. Teeth in the control group were treated with conventional root canal treatment, while teeth in the experimental group were treated with precise minimally invasive root canal treatment.

[Effects of MTA, iRoot SP and AH Plus on proliferation and differentiation of human periodontal ligament stem cells].

Purpose: To explore the effects of MTA, iRoot SP and AH Plus on periodontal ligament stem cells.

Methods: The periodontal ligament stem cells were cloned by limiting dilution culture method. The effects of MTA, iRoot SP and AH Plus on proliferation and apoptosis of periodontal ligament stem cells were detected by MTT and Annexin-V-FITC/PI double staining.

Diffusion kurtosis imaging assessment of the response to radiotherapy in a VX2 bone tumor model: an animal study.

Background: Neoadjuvant radiotherapy plays a vital role in the treatment of malignant bone tumors, and non-invasive imaging methods are needed to evaluate the response to treatment.

Purpose: To assess the value of diffusion kurtosis imaging (DKI) for monitoring early response to radiotherapy in malignant bone tumors.

Material And Methods: Treatment response was evaluated in a rabbit VX2 bone tumor model (n = 35) using magnetic resonance imaging (MRI), DKI, and histopathologic examinations.

miR-133b has protective effect on rats with acute lung injury caused by severe acute pancreatitis through targeting sp1 gene.

Objective: We aimed to investigate the regulatory mechanism of miR-133b and Sp1 in rats with severe acute pancreatitis complicated by acute lung injury.

Methods: The rats were divided into normal, NC, model, si-Sp1, miR-133b mimic, miR-133b inhibitor, and miR-133b inhibitor + si-Sp1 group and received different treatments.

Results: Compared with normal mice, model mice had a lower miR-133b expression, but higher levels of Sp1 expression, W/D of lung tissue, myeloperoxidase activities, and higher levels of interleukin(IL)-6, tumor necrosis factor (TNF)-α and IL-1β, cell apoptosis rate and Notch-1, and Hes-1, nuclear factor (NF)-κB P65 expressions in lung tissue.

Inhibition of mTORC1 in the rat condyle subchondral bone aggravates osteoarthritis induced by the overly forward extension of the mandible.

The present study aimed to investigate the role of mammalian target of rapamycin complex 1 (mTORC1) in the remodeling of the condyle subchondral bone in rats with temporomandibular joint osteoarthritis (TMJ OA) and explore the mechanisms involved. In this study, we used rats fitted with appliances to overly extend the mandible forward as an animal model of TMJ OA. Bone samples were collected 2, 4, and 8 weeks after appliance fixation.

The inhibition of BRAF activity sensitizes chemoresistant human ovarian cancer cells to paclitaxel-induced cytotoxicity and tumor growth inhibition.

Ovarian cancer is one of the most common cancers in women and the second most common cause of gynecologic cancer death in women worldwide. While ovarian cancer is highly heterogeneous in histological subtypes and molecular genetic makeup, epithelial ovarian cancer is the most common subtype. The clinical outcomes of ovarian cancer largely depend on early detection and access to appropriate surgery and systemic therapy.

Effect of water immersion restraint stress on gastric mucosa in rats with removed salivary glands.

Objective: To explore the effect of salivary gland removal on water immersion restraint stress (WIRS)-induced gastric mucosal injury (GMI) in rats.

Methods: Eighty male WISTAR rats were allocated into sham operation (sham) group, sham + WIRS group, salivary gland removal group, and salivary gland removal + WIRS group, with 20 rats in each group. In the sham group, skin and subcutaneous tissues were cut to expose glands.

Primary mucinous cystadenocarcinoma of the breast: a clinicopathologic analysis of one case and review of the literature.

Objective: To explore the clinicopathologic features and differential diagnosis of breast primary mucinous cystadenocarcinoma (MCA).

Methods: Pathological characteristics and immunophenotype of one case of MCA were analyzed. Literature was reviewed.

Blockade of IGF/IGF-1R signaling axis with soluble IGF-1R mutants suppresses the cell proliferation and tumor growth of human osteosarcoma.

Primary bone tumor, also known as osteosarcoma (OS), is the most common primary malignancy of bone in children and young adults. Current treatment protocols yield a 5-year survival rate of near 70% although approximately 80% of patients have metastatic disease at the time of diagnosis. However, long-term survival rates have remained virtually unchanged for nearly four decades, largely due to our limited understanding of the disease process.

[Related factors of atrophic glossitis in 124 consecutive cases].

Purpose: To study the relationship between atrophic glossitis and anemia, anemia types and other related factors(oral candida infection, xerostomia) in 124 consecutive cases.

Methods: One hundred and twenty-four cases with atrophic glossitis and 53 healthy controls were collected from Qingdao local population. The main indexes including general status, oral examination findings, hemoglobin (Hb), mean red blood cell volume (MCV), vitamin B12, ferritin, folic acid, anemia and anemia type, xerostomia and candida infection were statistically analyzed using SPSS 20.

[Expression and significance of chemokines CCL21, E-selectins and Hsp90 in periodontal tissues of rats with experimental periodontitis].

Purpose: To investigate the expression and significance of chemokines CCL21, E-selectins and heat shock protein 90 (Hsp90) in periodontal tissues of rats with experimental periodontitis.

Methods: Forty 10-week-old male Wistar rats were significantly randomly divided into 4 groups with 10 rats in each group. Periodontitis models were established in groups A, B and C, and the rest were 10 blank control groups.

[Autophagy induced by cyclic mechanical stretch inhibited caspase-dependent apoptosis of myoblasts].

Purpose: The aim of this study was to investigate the molecular mechanism of autophagy and apoptosis induced by cyclic mechanical stretch and the potential role of autophagy in stretch-induced apoptosis of myoblasts.

Methods: Loading model of L6 myoblasts was established in vitro. The cells were then subjected to cyclic mechanical stretch involving 3 s of 15% stretch alternating with 3 s of relaxation.

Blockade of CXCR2 suppresses proinflammatory activities of neutrophils in ulcerative colitis.

Ulcerative colitis (UC) is one chronically remittent and progressive inflammatory disorder. Chemokine receptor CXCR2 is reported to be involved in the pathogenesis of several inflammatory diseases. However, how CXCR2 modulate mucosal inflammation in UC is still obscure.

Plasma amyloid, tau, and neurodegeneration biomarker profiles predict Alzheimer's disease pathology and clinical progression in older adults without dementia.

Introduction: Plasma markers have been reported to be associated with brain amyloid burden, tau pathology, or neurodegeneration. We aimed to evaluate whether plasma biomarker profiles could predict Alzheimer's disease (AD) pathology and clinical progression in older adults without dementia.

Methods: Cross-sectional and longitudinal data of participants enrolled in this study were from the Alzheimer's Disease Neuroimaging Initiative (ADNI).

MSD-Net: Multi-Scale Discriminative Network for COVID-19 Lung Infection Segmentation on CT.

Since the first patient reported in December 2019, 2019 novel coronavirus disease (COVID-19) has become global pandemic with more than 10 million total confirmed cases and 500 thousand related deaths. Using deep learning methods to quickly identify COVID-19 and accurately segment the infected area can help control the outbreak and assist in treatment. Computed tomography (CT) as a fast and easy clinical method, it is suitable for assisting in diagnosis and treatment of COVID-19.

Targeted p21 activation by a new double stranded RNA suppresses human prostate cancer cells growth and metastasis.

We have previously demonstrated that miR-1236-3p and its sequence homology dsRNA, dsRNA-245 (which is completely complementary to the p21 promoter) had potential ability to upregulate p21 expression by targeting specific promoter sequence and inhibited bladder cancer (BCa). However, we still know little about the effect of miR-1236-3p on prostate cancer and which dsRNA has an inhibitory effect on prostate cancer (PCa)? Here, we confirmed that miR-1236-3p was decreased in PCa cells and tissues. MiR-1236-3p inhibited PCa cells growth and metastasis by activating p21.

Effects of 2,3,7,8-Tetrachlorodibenzo-p-dioxin on T Cell Differentiation in Primary Biliary Cholangitis.

Exposure to dioxins, such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), is reported to affect the autoimmune system and increase the risk of autoimmune disease. Generally, dioxin exerts its toxicity via aryl hydrocarbon receptor (AhR). Primary biliary cholangitis (PBC) is a chronic autoimmune disease, and its pathogenesis involves the interplay between immune and environmental factors.

Clinical and biomarker trajectories in sporadic Alzheimer's disease: A longitudinal study.

Introduction: Amyloid beta (Aβ) deposition was identified to precede tau pathology and neurodegeneration in familial Alzheimer's disease (AD). But the divergence between sporadic and familial AD limits the extension of these findings to sporadic AD.

Methods: Longitudinal changes of biomarkers among different stages were assessed using linear mixed-effects models.

Erratum: Magnetic resonance imaging monitoring therapeutic response to dendritic cell vaccine in murine orthotopic pancreatic cancer models.

[This corrects the article on p. 562 in vol. 9, PMID: 30949410.

Quantitative Analysis of Enhanced Computed Tomography in Differentiating Cystitis Glandularis and Bladder Cancer.

Objective: This study was performed to assess the value of quantitative analysis of enhanced computed tomography (CT) values in the differential diagnosis of bladder cancer and cystitis glandularis (CG).

Methods: Eighty patients with bladder masses (39 with CG and 41 with bladder cancer) who underwent enhanced CT were retrospectively reviewed. The CT enhancement values of the lesion and normal bladder wall in the arterial phase, venous phase, and delayed phase were measured.