Mutation analysis of the gene in Chinese patients with Dubin-Johnson syndrome.

Dubin-Johnson syndrome (DJS) is a rare, autosomal recessive disorder characterized by predominantly conjugated hyperbilirubinemia, caused by a mutation in the adenosine triphosphate-binding cassette subfamily C member 2 () gene coding the multidrug resistance-associated protein 2 (MRP2) protein. mutations have been identified in patients with DJS worldwide; however, the mutation pattern of in China is not well studied. In the present study, the mutation pattern of the gene in Chinese patients with DJS was investigated.

Molecular alterations of the NF2 gene in hepatocellular carcinoma and intrahepatic cholangiocarcinoma.

Neurofibromatosis type 2 with mutations in the neurofibromin 2 (NF2) gene, encoding the Merlin protein, is an autosomal dominant disorder characterized by enhanced cancer predisposition, particularly tumors of the central nervous system. Recent animal studies indicate that disruption of NF2/Merlin function in oval cells, which are hepatic progenitor cells, may lead to the development of primary liver cancers including hepatocellular carcinoma (HCC) and intrahepatic cholangiocarcinoma (ICC); however, its role in human primary liver cancer remains unclear. In the present study, we explored the role of NF2/Merlin in human primary liver cancers.

Are home visits an effective method for diabetes management? A quantitative systematic review and meta-analysis.

Aims/introduction: Previous reviews have revealed uncertainty regarding the effectiveness of home visit interventions for managing diabetes. Therefore, we carried out a quantitative systematic review and meta-analysis to evaluate the effects of home visit interventions among patients with diabetes.

Materials And Methods: We searched various electronic databases (PubMed, EMBASE, Cochrane Library, Web of Science, CINAHL, Wanfang and Chinese scientific full-text databases) from their inception until March 2016.

Identification and clinical significance of an elevated level of serum aminoacylase-1 autoantibody in patients with hepatitis B virus-related liver cirrhosis.

Chronic hepatitis B (CHB) is prevalent worldwide and can develop into liver cirrhosis and liver carcinoma. Early discrimination of liver cirrhosis from chronic hepatitis is critical for effective treatment and optimal prognosis. The aim of the present study was to assess the diagnostic value of a panel of cellular proteins that can be recognized by autoantibodies in patient serum for hepatitis B virus (HBV)‑related liver cirrhosis.

An Analysis of Immunoreactive Signatures in Early Stage Hepatocellular Carcinoma.

Background: Hepatocellular carcinoma (HCC) is prevalent worldwide and early diagnosis of HCC is critical for effective treatment and optimal prognosis.

Methods: Serum was screened first by immunoproteomic analysis for HCC-related tumor associated antigens (TAAs). Selected TAAs were clinically evaluated retrospectively in patients with HCC, liver cirrhosis, chronic hepatitis and healthy controls.

Genomic losses at 5q13.2 and 8p23.1 in dysplastic hepatocytes are common events in hepatitis B virus-related hepatocellular carcinoma.

Chromosomal loci with genomic imbalances are frequently identified in hepatocellular carcinoma (HCC). Greater than two-thirds of hepatitis B virus (HBV)-related HCCs originate from liver cirrhosis following a duration of up to two decades. However, it is unclear whether these genomic imbalances occur and accumulate in dysplastic hepatocytes of the cirrhotic liver during the progression from regenerated nodules to preneoplastic lesions, including dysplastic nodules (DN).

Ulnar artery compression: a feasible and effective approach to prevent the radial artery occlusion after coronary intervention.

Background: Radial artery (RA) occlusion (RAO) is not rare in patients undergoing coronary intervention by transradial approach (TRCI). Predictors of and prevention from RAO have not been systematically studied. This study aimed to analyze the risk factors of the weakness of RA pulsation (RAP) and its predictive value for RAO after TRCI, and simultaneously to describe a feasible and effective approach to maintain RA patency.

Esophageal squamous cell carcinoma and ALDH2 and ADH1B polymorphisms in Chinese females.

Aim: Alcohol dehydrogenase-IB (ADH1B) and aldehyde dehydrogenase-2 (ALDH2) are the key enzymes for elimination of ethanol and acetaldehyde, the latter being an established animal carcinogen produced after drinking. In this study, we aimed to evaluate the contribution of ADH1B and ALDH2 polymorphisms to the risk of esophageal squamous cell (ESCC) in Chinese females.

Methods: A total of 81 pathologically-proven female ESCC cases and 162 female controls were recruited from the Affiliated Hospital of Medical College of Armed Police Forces of PRC in China.

Mechanism and dose-effect of Ginkgolide B on severe acute pancreatitis of rats.

Aim: To determine the optimal dosage and mechanism of Ginkgolide B (BN52021) on severe acute pancreatitis (SAP) of rats.

Methods: Seventy male Wistar rats were randomly divided into seven groups (10 for each group). Sham-operation group (SO), SAP model group (SAP), dimethyl sulfoxide (DMSO) contrast group (DMSO), and groups treated with 2.

[Effect of collagen type II on redifferentiation of dedifferentiated rabbit chondrocytes].

Objective: Collagen type II is a characteristic molecular of chondrocyte. With continuous subculture of chondrocytes, they progressively lose the ability to express collagen type II. To observe the effect of collagen type II on redifferentiation of dedifferentiated rabbit chondrocytes so as to lay a experimental foundation for use of chondrocytes in cartilage tissue engineering.

[Relationship between red cell volume distribution width and cardiac function in acute coronary syndrome patients].

Objective: To evaluate the relationship between red cell volume distribution width (RDW) and cardiac function in acute coronary syndrome (ACS) patients.

Methods: We analyzed the baseline clinical data of 228 ACS patients consecutively admitted into our hospital according to the classification of RDW and NYHA function class. Correlation analysis was made respectively between RDW and plasma creatinine and left ventricular ejection fraction (LVEF).

Tropism mechanism of stem cells targeting injured brain tissues by stromal cell-derived factor-1.

Objective: To explore the role and function of stromal cell-derived factor-1 (SDF-1) in stem cells migrating into injured brain area.

Methods: Rat-derived nerve stem cells (NSCs) were isolated and cultured routinely. Transwell system was used to observe the migration ability of NSCs into injured nerve cells.

[Differential proteomics in glioblastoma].

Objective: To establish differential proteomics profiles of glioblastoma cell lines from Chinese, and to provide reference for future basic studies.

Methods: Total protein was extracted from 3 glioblastoma cell lines, CHG-5, TJ899 and TJ905. After normalization, the total protein was presented by two-dimensional (2D) electrophoresis, scanned and analyzed.

Stem cells modified by brain-derived neurotrophic factor to promote stem cells differentiation into neurons and enhance neuromotor function after brain injury.

Objective: To promote stem cells differentiation into neurons and enhance neuromotor function after brain injury through brain-derived neurotrophic factor (BDNF) induction.

Methods: Recombinant adenovirus vector was applied to the transfection of BDNF into human-derived umbilical cord mesenchymal stem cells (UCMSCs). Enzyme linked immunosorbent assay (ELISA) was used to determine the secretion phase of BDNF.

[Clinical efficacy of pishen bingbu recipe in patients with sympathetic cervical spondylosis and its impact on heart rate variability].

Objective: To investigate the clinical effect of Pishen Bingbu Recipe (PBR) in treating patients with sympathetic cervical spondylosis (SCS) of qi-blood deficient syndrome type and its impact on heart rate variability (HRV).

Methods: Fifty patients were randomized into the control group and the treatment group equally. Both were treated with mecobalamin, vitamin B1, neurotropin, and occipital - jaw band traction in the sitting posture, but to patients in the treatment group, PBR was given additionally.

[Preliminary study on chitosan/HAP bilayered scaffold].

Objective: To study repair of osteochondral defects by using composite of autologous BMSCs and chitosan/HAP (CS/HAP) bilayered scaffold in rabbits and its feasibility as osteochondral tissue engineering scaffolds.

Methods: CS/HAP bilayered scaffolds were produced with CS and HAP using a lyophilization and sintering method. The pore size of the scaffold was observed by scanning electron microscopy (SEM).

[Surgical techniques of open reduction and internal fixation for ankle fracture].

Objective: To evaluate the surgical techniques of open reduction and internal fixation performed for ankle fracture retrospectively and the corresponding clinical outcomes.

Methods: From March 2001 to January 2006, 242 patients with ankle fracture were treated. A total of 85 patients with complete clinical data were analyzed.

[Therapeutic effect evaluation of artificial hip replacement].

Objective: To discuss the indications and other related issues of artificial hip replacement.

Method: A retrospective analysis was conducted in 37 cases with artificial hip replacement with complete follow-up data treated between 1996 and 2001.

Results: The patients were followed up for 1 to 5 years.