Evaluation of the diagnostic potential and DIVA capability of recombinant LigBCon1-5 protein of serovar Pomona in canine leptospirosis.

Background: Canine leptospirosis is a serious public health concern.

Aims: This study aims to investigate the feasibility of conserved first to fifth domains of recombinant immunoglobulin like protein B antigen (rLigBCon1-5) as a serodiagnostic marker for detecting canine leptospirosis.

Methods: A total of 340 unvaccinated canine serum samples were screened using microscopic agglutination test (MAT) and rLigBCon1-5 based immunoglobulin G (IgG) indirect-enzyme-linked immunosorbent assay (I-ELISA).