Insights into A3(2) growth and pigment formation with high-throughput online monitoring.

species are intensively studied for their ability to produce a variety of natural products. However, conditions influencing and leading to product formation are often not completely recognized. Therefore, in this study, high-throughput online monitoring is presented as a powerful tool to gain in-depth understanding of the cultivation of the model organism A3(2).

Online monitoring applying the anaerobic respiratory monitoring system reveals iron(II) limitation in YTF medium for .

Online monitoring of microbial cultures is an effective approach for studying both aerobic and anaerobic microorganisms. Especially in small-scale cultivations, several parallel online monitored experiments can generate a detailed understanding of the cultivation, compared to a situation where a few data points are generated from time course sampling and offline analysis. However, the availability of small-scale online monitoring devices for acetogenic organisms is limited.

Online monitoring of the respiratory quotient reveals metabolic phases during microaerobic 2,3-butanediol production with .

Microaerobic cultivation conditions are often beneficial for the biotechnological production of reduced metabolites like 2,3-butanediol. However, due to oxygen limitation, process monitoring based on oxygen transfer rate, or dissolved oxygen measurement provides only limited information. In this study, online monitoring of the respiratory quotient is used to investigate the metabolic activity of DSM 8785 during mixed acid-2,3-butanediol production under microaerobic conditions.