High-risk human papillomavirus in oral squamous cell carcinoma of young patients.

The aim of this study was to investigate a possible relation between oral squamous cell carcinoma (SCC), the presence of high-risk human papillomavirus (HR-HPV) DNA and p16 expression in young patients. Paraffin-embedded tumor blocks from 47 oral SCC of young (≤40-year old) patients were evaluated. The presence of HPV DNA in tumor specimens was analyzed by polymerase chain reaction (PCR) using GP5+/GP6+ generic primers (L1 region) followed by dot blot hybridization for HPV typing.

Enhanced neural progenitor/stem cells self-renewal via the interaction of stress-inducible protein 1 with the prion protein.

Prion protein (PrP(C) ), when associated with the secreted form of the stress-inducible protein 1 (STI1), plays an important role in neural survival, neuritogenesis, and memory formation. However, the role of the PrP(C) -STI1 complex in the physiology of neural progenitor/stem cells is unknown. In this article, we observed that neurospheres cultured from fetal forebrain of wild-type (Prnp(+/+) ) and PrP(C) -null (Prnp(0/0) ) mice were maintained for several passages without the loss of self-renewal or multipotentiality, as assessed by their continued capacity to generate neurons, astrocytes, and oligodendrocytes.

CCND1 amplification and protein overexpression in oral squamous cell carcinoma of young patients.

Background: The purpose of this study was to evaluate the correlation of CCND1 amplification and protein overexpression with clinicopathological features and clinical outcomes in patients younger than 41 years old with oral squamous cell carcinoma (SCC).

Methods: Eighty-six young patients with oral SCC were evaluated using the tissue microarray technique, immunohistochemistry, and fluorescence in situ hybridization (FISH). These cases were compared with 116 patients with oral cancer aged over 50 years old (controls).

Portal vein obstruction after liver transplantation in children treated by simultaneous minilaparotomy and transhepatic approaches: initial experience.

Portal vein thrombosis is a complication that occurs anytime after liver transplantation and can compromise the patient and graft survival. We describe a combined technique for PV recanalization in cases of PV obstruction after liver transplantation. Four children (1%), of 367 subjected to liver transplantation from June 1991 to December 2008, underwent PV recanalization through a combined approach (transhepatic and minilaparotomy).

Primary monophasic synovial sarcoma of the pleura: Neoadjuvant chemotherapy followed by complete resection.

We describe a rare case of primary pleural synovial sarcoma in a 27-year-old man with a 4-month history of dry cough and left-sided chest pain. A CT scan showed a large cystic mass in the left pleural cavity. The patient underwent two video-assisted thoracoscopic biopsies and the diagnosis of synovial sarcoma of the pleura was established.

Impact of modified radical neck dissections on the number of retrieved nodes, recurrence and survival.

Unlabelled: Neck dissection is an integral part of head and neck tumors. Throughout its history, it has undergone changes looking for an improvement in functional outcome without loss of oncologic efficiency.

Aim: Demonstrate that the modified radical neck dissection have an oncologic results comparable to classical radical dissection.

ErbB receptors and fatty acid synthase expression in aggressive head and neck squamous cell carcinomas.

Summary: Overexpression of ErbB receptors is frequent in head and neck squamous cell carcinomas (HNSCC) and seems to be correlated with tumor progression and metastasis. Fatty acid synthase (FASN), the key lipogenic enzyme responsible for the endogenous synthesis of fatty acids, is regulated by ErbB2 and overexpressed in several human malignancies.

Methods: This study was performed to examine the immunohistochemical expression patterns of ErbB1, ErbB2, ErbB3, ErbB4, and FASN in a tissue microarray, containing 33 representative areas from aggressive primary HNSCC (whose patients had distant metastasis), and 21 matched lung metastasis.

Total glossectomy in the organ preservation era.

Purpose Of Review: Total or subtotal glossectomy is considered a highly morbid procedure. However, in some institutions and in selected cases of unsuccessful chemoradiation regimens, it is performed with acceptable oncological and functional outcomes.

Recent Findings: Chemoradiation for advanced oral and oropharyngeal cancers is also highly morbid and some authors reported reasonable functional results after major glossectomies.

Evaluation of quantitative rt-PCR using nonamplified and amplified RNA.

Quantitative reverse-transcription polymerase chain reaction (qRT-PCR) is a standard assay in molecular medicine for gene expression analysis. Samples from incisional/needle biopsies, laser-microdissected tumor cells and other biologic sources, normally available in clinical cancer studies, generate very small amounts of RNA that are restrictive for expression analysis. As a consequence, an RNA amplification procedure is required to assess the gene expression levels of such sample types.

Case-control study on prognostic factors in oral squamous cell carcinoma in young patients.

Background: Oral squamous cell carcinoma affects mainly patients between the fifth and sixth decades of life, being rare in the young (≤40 years old).

Methods: Demographic, clinical, and pathologic features, and the long-term survival of 125 patients younger than 41 years of age were compared with 250 control patients older than 50 years old. Data were submitted to Kaplan-Meier and log-rank tests.

Characteristics associated with complete surgical resection of primary malignant mediastinal tumors.

Objective: To identify preoperative characteristics associated with complete surgical resection of primary malignant mediastinal tumors.

Methods: Between 1996 and 2006, 42 patients with primary malignant mediastinal tumors were submitted to surgery with curative intent at a single facility. Patient charts were reviewed in order to collect data related to demographics, clinical manifestation, characteristics of mediastinal tumors and imaging aspects of invasiveness.

Assessment of pulmonary function and quality of life in patients submitted to pulmonary resection for cancer.

Objective: To evaluate the effects that pulmonary resection has on pulmonary function and quality of life (QoL) in patients with primary or metastatic lung cancer.

Methods: This was a prospective cohort study involving all patients submitted to pulmonary resection for cancer between September of 2006 and March of 2007 at the A. C.

Multiple granular cell tumors of the tongue and parotid gland.

Parotid granular cell tumor (GCT) is a very rare tumor, with only 11 previously reported cases in the English-language literature. This report describes an uncommon case of multiple GCT involving the tongue and parotid gland and provides a review of the literature. A 58-year-old woman presented with a slow-growing mass involving the parotid region, which measured 3.

Dental extractions related to head and neck radiotherapy: ten-year experience of a single institution.

Objective: This study evaluates the frequency of osteoradionecrosis associated with dental extractions.

Study Design: A total of 405 patients submitted to radiotherapy and had dental extractions were evaluated. The patients were divided into 3 groups.

Factors associated with location of death of children with cancer in palliative care.

Objective: The aim of this study was to identify factors associated with location of death of patients receiving palliative care in a pediatric oncology unit.

Methods: A palliative care program was developed in the pediatric department in order to provide specialized attention to the patient and family in end-of-life. The program is coordinated by a nurse, delivering a simultaneous interdisciplinary team approach with focus on identification and training of a family care provider as well as local resources supplemented by support of a social worker and the community.

Antigen retrieval methods and estrogen receptor immunoexpression using 1D5 antibody: a comparative study.

Long-term formalin tissue fixation results in antigen masking, probably through aldehydic linkage between proteins and fixative molecules. Immunohistochemistry results depend on the type of the detection procedure and the type of antibody used for the reaction. Considering the difficulty in working with estrogen receptor (ER) antibodies and the lack of standardization of the antigen retrieval methods, we quantified the immunoexpression of ER using the 1D5 antibody and a standard streptavidin-biotin detection procedure retrieving with microwave oven, steamer, pressure cooker, and water bath in a set of SBR grade 2 invasive breast carcinomas.

Periodontal changes in patients undergoing radiotherapy.

Background: The purpose of this study was to evaluate changes in the periodontium in patients who received head and neck radiation therapy.

Methods: Periodontal clinical parameters (probing depth, clinical attachment level, gingival recession, plaque index, and bleeding on probing) were assessed on 27 patients before and 6 to 8 months following radiation therapy in the head and neck area.

Results: The greatest changes occurred in clinical attachment level: overall, 70.

Management of chemo- and radiotherapy induced oral mucositis with low-energy laser: initial results of A.C. Camargo Hospital.

Background. Oral mucositis is a common complication of some malignancies treatment, causing therapeutic modifications due to patient's debilitation, which often interferes with the prognosis of the disease. Many attempts have been made to find an optimal treatment or preventive method to minimize the severity of oral mucositis.

Needle displacement during high-dose-rate afterloading brachytherapy boost and conventional external beam radiation therapy for initial and local advanced prostate cancer.

Purpose: To determine the displacement of needles (ND) and its impact in the outcome of initial and locally advanced prostate cancer treated with conventional external beam radiation therapy (EBRT) and fractionated transrectal ultrasound-guided high-dose-rate afterloading brachytherapy (HDR-BT) as a boost.

Methods And Materials: From 03/97 to 08/98 a total of 47 patients were eligible for study entry. Patients with 1992 AJCC clinical stage T3a or less and prostatic volume View Article and Find Full Text PDF

Predictive factors for diagnosis of advanced-stage squamous cell carcinoma of the head and neck.

Objective: To identify the predictive factors (with emphasis on diagnostic delay) associated with the diagnosis of an advanced-clinical stage head and neck cancer.

Design: Cross-sectional study of patients with head and neck cancer originally recruited for a case-control study.

Setting: Three referral oncological centers in metropolitan areas in southern Brazil: São Paulo, Curitiba, and Goiânia.

Radiation and chemotherapy instead of surgery for low infiltrative rectal adenocarcinoma: a prospective trial.

Background: The objective of this prospective study was to determine the possibility of treatment based exclusively on chemotherapy and radiotherapy for patients with low infiltrative rectal tumors in an attempt to preserve sphincter function.

Methods: Sixteen patients with rectal adenocarcinoma up to 3 cm above the pectineal line with initial indications for abdominoperineal resection (APR) were submitted to a 5040-cGy (28 x 180 cGy) radiotherapy dose and chemotherapy during the first 3 and last 3 days of radiotherapy, using 425 mg/m2/day of 5-fluorouracil (5FU) and 20 mg/m2/day of folinic acid. Levamisole was used at 150 mg/day for 3 consecutive days at 2-week intervals throughout the period of therapy.

Experience with six children with fetal rhabdomyomatous nephroblastoma: review of the clinical, biologic, and pathologic features.

Background: Fetal rhabdomyomatous nephroblastoma (FRN) is a rare variant of Wilms tumor.

Materials And Results: One hundred and thirty two children with kidney tumors were seen at our hospital from 1985 to 1993. Among them were 6 (4.

Internal hemipelvectomy in the treatment of recurrent carcinoma of the colon: report of a case.

Purpose: Although extended surgery has been established as an effective method for the treatment of advanced carcinoma of the colon, there are no reports in the literature of en bloc resection of the tumor together with the iliac bone. We report herein a 46-year-old woman with a second local recurrence after right colectomy, with the main objective of showing the possibility of indicating this type of surgery in selected cases.

Methods: In view of the lack of therapeutic options for the case and the absence of metastases, extended curative surgery for recurrent carcinoma of the colon was performed, with en bloc resection of the right iliac bone and of the crural nerve (Type I internal hemipelvectomy).