miR158a negatively regulates plant resistance to Phytophthora parasitica by repressing AtTN7 that requires EDS1-PAD4-ADR1 complex in Arabidopsis thaliana.

Small RNAs are involved in diverse cellular processes, including plant immunity to pathogens. Here, we report that miR158a negatively regulates plant immunity to the oomycete pathogen Phytophthora parasitica in Arabidopsis thaliana. By performing real-time quantitative PCR, transient expression, and RNA ligase-mediated 5' rapid amplification of cDNA ends assays, we demonstrate that miR158a downregulates AtTN7 expression by cleaving its 3'-untranslated region.

The Phytophthora infestans effector Pi05910 suppresses and destabilizes host glycolate oxidase StGOX4 to promote plant susceptibility.

Phytophthora infestans is a notorious oomycete pathogen that causes potato late blight. It secretes numerous effector proteins to manipulate host immunity. Understanding mechanisms underlying their host cell manipulation is crucial for developing disease resistance strategies.

Utilization of Assisted Reproductive Technologies in Breeding Auliekol Cattle: A Comparative Study.

This study evaluates the utilization of in vitro embryo production (IVEP) technology for the conservation and breeding of the Auliekol cattle breed, a primary beef breed in Kazakhstan facing population decline due to the cessation of breeding programs and the incursion of transboundary diseases. We assessed the effect of consecutive ovum pick-up (OPU) procedures on oocyte yield and embryo production in Auliekol and Aberdeen Angus cows. A total of 2232 and 3659 oocytes were aspirated from Auliekol and Aberdeen Angus donors, respectively, with significantly higher yields and embryo production observed in Aberdeen Angus cows.

Improving the level of water quality and plant species diversity in the reservoir accumulating natural effluents from the reclaimed uranium-containing industrial waste dump.

Due to the need to achieve the principles of sustainable development and to understand the processes of formation of phytocenoses in areas that were adversely affected by the industrial impact, this study assessed the condition of the Grachevsky uranium mine (Kazakhstan), which underwent conservation procedures about 25 years ago. The purpose is to determine the level of water quality and phytocenosis of the shores of the reservoir accumulating natural effluents from reclaimed dumps and anthropogenic sites of a uranium mine, as well as quality indicators and toxicology. The assessment included a qualitative research method (analysis of documents) to determine agro-climatic conditions and empirical methods of collecting information.

Contamination of Kazakhstan cheeses originating from and its resistance to antimicrobial drugs.

Background And Aim: , a commensal intestine bacterium of vertebrates, is widely distributed in the environment and indicates the microbiological quality of food products in relation to coliforms. In addition, virulent strains, particularly O157:H7, cause outbreaks of toxic infections caused by consuming dairy products. Because food safety studies regarding have not been conducted in Central Asia, this research aimed to study the characteristics of contamination, microbiological and genotypic properties, and resistance to antimicrobial agents of strains that contaminate various types of commercialized cheeses originating from Kazakhstan.

Prevalence and antimicrobial resistance of Enterobacteriaceae in the north of Kazakhstan.

Background: An increasing number of drugs are used each year in the treatment of small pets (cats and dogs), including medicines (cephalosporins and fluoroquinolones) used in human therapy.

Aim: The purpose of this study was to isolate and explore the antibiotic resistance of opportunistic Enterobacteriaceae (, ) from cats and dogs, and to isolate resistance genes in the microorganisms.

Methods: In 2021, 808 samples of biological material from small domestic animals were collected in veterinary clinics in Kostanay.

Assessment of the state of vegetation cover of recultivated dumps of uranium deposits in Northern Kazakhstan.

The purpose of this study was to assess the processes of plant community formation on recultivated dumps of spent uranium-containing industrial waste from uranium deposit mines, as well as to identify the degree of impact of agro-climatic factors, agrochemical indicators of soils of recultivated dumps, and the level of residual ionizing radiation on the productivity of the emerging vegetation cover. Studies of plant colonization of recultivated Grachevsky and Shantobinsky uranium mine dumps located in Northern Kazakhstan were carried out. The mining and technical stage of reclamation consisted of planning a dump with slopes of 15° and covering it with a 1 m layer of chestnut soil.

Benefits of automated pig feeding system: A simplified cost-benefit analysis in the context of Kazakhstan.

Background And Aim: Automated pig feeding system is an emerging technology with the potential to considerably enhance pig farming. This study aimed to explore the benefits of automated pig feeding systems and provide a simplified cost-benefit analysis, which would serve as a valuable decision-making tool for the stakeholders.

Materials And Methods: This study conducted a literature review of automated pig feeding systems and explored recent advancements.

The influence of lactic acid bacteria on the viability of the reference strain of Listeria monocytogenes 123 serotype I in plant foods.

Listeria (L.) monocytogenes is the causative agent of human listeriosis, the frequent sourceof which is food of animal origin. The aim of this study was to determine the influence of lactic acid bacteria (LAB) on the viability of Listeria in carrot juice and compound feed inoculated with L.

Quantitative assessment of soil condition, basic environmental factors and productivity of Linum usitatissimum in the steppe zone of Kazakhstan using the remote sensing method.

The influence of environmental factors, such as lack of water and uneven rainfall, depletion of nutrients in the soil and reduced soil fertility, planting patterns and plant density, uneven growth stages, are the main limiting factors that hinder the growth of agricultural production in arid regions. The aim of the study was to assess the potential of Sentinel-2 to quantify soil conditions, which can improve the understanding of spatiotemporal dynamics in organic agriculture in the steppe zone of Kazakhstan and improve productivity management of Linum usitatissimum. In the course of the research, the influence of individual factors of the general environmental impact, such as the influence of humidity, meteorological conditions, the content of individual nutrient components of the soil on the yield, was studied.

Phenotypic and genotypic resistance to antibiotics in strains isolated from cattle milk in Northern Kazakhstan.

Background And Aim: is the most frequent and ubiquitous cause of mastitis in cows. In recent decades, antibiotic resistance has rapidly spread among infectious disease pathogens in Kazakhstan and globally. This study examined the phenotypic and genotypic resistance of .

Identification of the causative agent of canine babesiosis in the North of Kazakhstan.

Background: Canine babesiosis is a common disease in the northern part of the Republic of Kazakhstan, in particular in the Kostanay region. In recent years, a large number of cases of the disease with a variety of clinical symptoms have been registered.

Aim: The purpose of the study was to monitor the spread, characterization, and identify the species involved of species in ticks and blood of dogs in the Kostanai region.

Problem of Power in Michel Foucault's Philosophy.

For many centuries, the issues of power, politics, and state have been widely discussed in scientific literature. Developing theories, philosophers proceeded from the side of hierarchical affiliation of power, which is confirmed in theological and biological concepts. Relevance of the presented article is conditioned by the fact that for the first time the French philosopher M.

In Vitro Fertilization in Kazakh Whiteheaded Cattle: A Comparative Study.

In vitro fertilization (IVF) technologies have great potential in the preservation of endangered species. In the current study, an IVF experiment was carried out to evaluate whether reproductive technologies are suitable for Kazakh Whiteheaded cattle, aimed at preserving this breed whose population has reduced drastically over the last thirty years. The reproduction characteristics of Kazakh Whiteheaded cows were compared to Aberdeen Angus cows.

Review of Orchidaceae of the northern part of Kazakhstan.

We present a review of Orchidaceae Juss. of the northern part of Kazakhstan, within the steppe, forest-steppe and semi-desert habitats of the country (Pavlodar, northern Kazakhstan, Kostanay, Akmola, Aktobe, West Kazakhstan, partially Karaganda and East Kazakhstan regions). The investigation is based on herbarium materials, literature data and field observations.

Interactions of piRNAs with the mRNA of Candidate Genes in Esophageal Squamous Cell Carcinoma.

Recently, a database of human piRNAs (piwi-interacting RNAs) was created, which allows the study of the binding of many piRNAs to the mRNAs of genes involved in many diseases, including cancer. In the present work, we identified the piRNAs that can interact with candidate esophageal squamous cell carcinoma (ESCC) genes. The binding of 480 thousand piRNAs with the mRNAs of 66 candidate ESCC genes was studied.

Endogenous piRNAs Can Interact with the Omicron Variant of the SARS-CoV-2 Genome.

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which caused the COVID-19 pandemic, can still infect populations in many countries around the globe. The Omicron strain is the most mutated variant of SARS-CoV-2. The high transmissibility of the strain and its ability to evade immunity necessitate a priority study of its properties in order to quickly create effective means of preventing its spread.

Prevalence and resistance to antibacterial agents in strains isolated from poultry products in Northern Kazakhstan.

Background And Aim: is one of the main causative agents of foodborne infections. The source of the pathogen, in most cases, is poultry products. The intensification of poultry farming and the constant and uncontrolled use of antimicrobials has led to an increase in the level of antibiotic resistance, especially in developing countries.

Knowledge, attitude and practice (KAP) of smallholder farmers on foot-and-mouth disease in Cattle in West Kazakhstan.

Background: This study was performed to assess the knowledge, attitudes and practices (KAPs) of farmers and veterinary professionals towards foot-and-mouth disease (FMD) in the area studied.

Methods: The study was based on a comprehensive questionnaire administered through face-to-face interviews. Between January and May 2022, 543 households and 27 animal health practitioners (AHP) were visited in 4 provinces of the West Kazakhstan region to assess their KAPs towards FMD.

Genetic polymorphism of prolactin and nitric oxide synthase in Holstein cattle.

Background And Aim: Bacterial and viral infections affect the welfare of animals and lead to large economic losses in dairy cattle breeding due to decreased productive indicators and increased culling rates. In modern dairy farming, farmers are looking for effective solutions to prevent and minimize infectious disease risks. To this end, the most relevant study field is the search for gene sites that impact production and health.

piRNA and miRNA Can Suppress the Expression of Multiple Sclerosis Candidate Genes.

Multiple sclerosis (MS) is a common inflammatory demyelinating disease with a high mortality rate. MS is caused by many candidate genes whose specific involvement has yet to be established. The aim of our study was to identify endogenous miRNAs and piRNAs involved in the regulation of MS candidate gene expression using bioinformatic methods.

Meta-analysis of the potential of Kazakhstani pork in the global market of meat products: Problems and prospects.

Background And Aim: Pig farming is integral in developing Kazakhstan's animal husbandry as it has shown rapid growth in profit and a high turnover. Industrial pig products have significant global demand, particularly in China and Russia. However, as pig farming is a small-scale industry with insufficient mechanization and automation in Kazakhstan, the costs have increased while the quality of Kazakhstani pig products has decreased due to the simultaneous influx of cheap imported pork into the domestic market.

Self-reported consumption frequency of meat and fish products among young adults in Kazakhstan.

Meat and dairy products are important ingredients in Kazakhstan, although there are indications that high consumption of red and processed meat is associated with a risk of several non-communicable diseases and has an adverse impact on the environment. The aim of this study was to investigate the dietary habits of young adults in Kazakhstan, particularly meat and fish consumption frequency among university students in five regions of Kazakhstan. The assessment of meat and fish consumption was based on the food frequency questionnaire.

An intelligent testing system development based on the shingle algorithm for assessing humanities students' academic achievements.

Computer-based testing of humanities students has some inconveniences and difficulties, where the whole learning process is practically based on communicative methods. In this regard, one needs such a testing system, which would allow one to ask open-ended questions, and students would be able to enter detailed answers. Despite the popularity of using the shingle algorithm in determining plagiarism, few researchers have attempted to use it in assessing the academic achievements of students.