From single cells to complex tissues in applications of surface-enhanced Raman scattering.

As surface-enhanced Raman scattering (SERS) continues to grow in popularity, more work needs to be done to evaluate its compatibility with a wider scope of applications. With such a strong emphasis on SERS being used for biosensing, it is important to examine how SERS is used in bioanalytical nanoscience, and more importantly, look towards where SERS is heading. For many, the initial steps involve demonstrating in vivo sensing by SERS using cultures of live cells.

A blueprint for performing SERS measurements in tissue with plasmonic nanofibers.

Plasmonic nanostructures have found increasing utility due to the increased popularity that surface-enhanced Raman scattering (SERS) has achieved in recent years. SERS has been incorporated into an ever-growing list of applications, with bioanalytical and physiological analyses having emerged as two of the most popular. Thus far, the transition from SERS studies of cultured cells to SERS studies involving tissue has been gradual and limited.

A high-throughput plasmonic tongue using an aggregation assay and nonspecific interactions: classification of taste profiles in maple syrup.

A simple colorimetric test detects off-flavour profiles of maple syrups in minutes, which are detectable by the naked eye. As flavour profiles are due to complex mixtures of molecules, the test uses nonspecific interactions for analysing the aggregation and color change of Au nanoparticles (AuNPs) induced by the different organic molecules contained in off-flavour maple syrup. The test was optimal with 13 nm citrate-capped AuNPs reacting 1 : 1 with pure maple syrup diluted 10 times.

Systematic review and meta-analysis of olfactive stimulation interventions to manage procedural pain in preterm and full-term neonates.

Background: Preterm and full-term neonates undergo many painful procedures during their hospitalization in the neonatal intensive care unit. Unrelieved and repeated pain can have important repercussions on their motor and intellectual development. Still, pain management interventions are limited for neonates.

Branched Au Nanoparticles on Nanofibers for Surface-Enhanced Raman Scattering Sensing of Intracellular pH and Extracellular pH Gradients.

The development of plasmonic-active nanosensors for surface-enhanced Raman scattering (SERS) sensing is important for gaining knowledge on intracellular and extracellular chemical processes, hypoxia detection, and label-free detection of neurotransmitters and metabolites, among other applications in cell biology. The fabrication of SERS nanosensors for optophysiology measurements using substrates such as nanofibers with a uniform distribution of plasmonic nanoparticles (NPs) remains a critical hurdle. We report here on a strategy using block copolymer brush-layer templating and ligand exchange for fabricating highly reproducible and stable SERS-active nanofibers with tip diameters down to 60 nm and covered with well-dispersed and uniformly distributed branched AuNPs, which have intrinsic hotspots favoring inherently high plasmonic sensitivity.

Portable and field-deployed surface plasmon resonance and plasmonic sensors.

Plasmonic sensors are ideally suited for the design of small, integrated, and portable devices that can be employed in situ for the detection of analytes relevant to environmental sciences, clinical diagnostics, infectious diseases, food, and industrial applications. To successfully deploy plasmonic sensors, scaled-down analytical devices based on surface plasmon resonance (SPR) and localized surface plasmon resonance (LSPR) must integrate optics, plasmonic materials, surface chemistry, fluidics, detectors and data processing in a functional instrument with a small footprint. The field has significantly progressed from the implementation of the various components in specifically designed prism-based instruments to the use of nanomaterials, optical fibers and smartphones to yield increasingly portable devices, which have been shown for a number of applications in the laboratory and deployed on site for environmental, biomedical/clinical, and food applications.

Au nanoparticles as label-free competitive reporters for sensitivity enhanced fiber-optic SPR heparin sensor.

A label-free Au NPs-enhanced surface plasmon resonance (SPR) sensor was developed for the ultrasensitive detection of heparin based on competitive adsorption behavior of heparin and Au NPs on the poly (dimethyl-diallylammonium chloride) (PDDA)-modified optical fiber surface and the corresponding change in the resonance wavelength of SPR. Due to the high affinity between heparin and PDDA, the present senor shows good analytical performance with respect to heparin detection. Two obvious advantages of the proposed heparin sensor over other reported methods are: its much wider linear concentration range (10-10 g/mL) and lower limit of detection (0.

In Vitro Drug Release and Biocatalysis from pH-Responsive Gold Nanoparticles Synthesized Using Doxycycline.

pH-sensitive doxycycline gold nanoparticles (doxy-AuNPs) are reported here to act as effective drug nanocarriers and as biocatalysts. The AuNPs were synthesized with doxy as the reducing and capping agent. Various parameters were optimized to find the best conditions for the synthesis of doxy-AuNPs, and these were characterized with UV-vis, X-ray diffraction (XRD), FTIR, and transmission electron microscopy (TEM).

Understanding competing discourses as a basis for promoting equity in primary health care.

Background: Globally, health inequities persist with effects on whole populations and the most profound effects on populations marginalized by poverty, discrimination and other forms of disadvantage. In the current neoliberal political-economic context, health inequities are produced and sustained by the inequitable distribution of social determinants of health and structural inequities such as discrimination and institutional racism. Even in the context of healthcare organizations with an explicit commitment to health equity, multiple intersecting discourses, such as ongoing efficiency discourses, and culturalist and racialized discourses, are in constant interaction with healthcare practices at the point of care and the organizational level, limiting providers' and organizations' capacities to address structural inequities.

The Fundamentals of Real-Time Surface Plasmon Resonance/Electrogenerated Chemiluminescence.

We report the integration of surface plasmon resonance (SPR), cyclic voltammetry and electrochemiluminescence (ECL) responses to survey the interfacial adsorption and energy transfer processes involved in ECL on a plasmonic substrate. It was observed that a Tween 80/tripropylamine nonionic layer formed on the gold electrode of the SPR sensor, while enhancing the ECL emission process, affects the electron transfer process to the luminophore, Ru(bpy) , which in turn has an impact on the plasmon resonance. Concomitantly, the surface plasmon modulated the ECL intensity, which decreased by about 40 %, due to an interaction between the excited state of Ru(bpy) and the plasmon.

Optimisation modelling to improve the diets of First Nations individuals.

We examined the feasibility of linear programming (LP) to develop diets that were economical, included traditional (cultural, non-market) foods and met the dietary reference intakes (DRI) in a Canadian Indigenous population. Diet optimisation using LP is a mathematical technique that can develop food-based dietary guidelines for healthy eating in Indigenous populations where food insecurity, availability and cost are important considerations. It is a means of developing nutritionally optimal food combinations that are based on economical and culture-specific foods.

What are the factors associated with the implementation of a peer-led health promotion program? Insights from a multiple-case study.

Peer education is widely used as a health promotion strategy. However, few efforts have been undertaken to understand the implementation of peer-led health promotion programs (HPPs). This multiple-case study identifies factors facilitating the implementation of a peer-led HPP for older adults presenting with fear of falling (Vivre en �quilibre) and their mechanisms of action.

The NSERC Canadian Lake Pulse Network: A national assessment of lake health providing science for water management in a changing climate.

The distribution and quality of water resources vary dramatically across Canada, and human impacts such as land-use and climate changes are exacerbating uncertainties in water supply and security. At the national level, Canada has no enforceable standards for safe drinking water and no comprehensive water-monitoring program to provide detailed, timely reporting on the state of water resources. To provide Canada's first national assessment of lake health, the NSERC Canadian Lake Pulse Network was launched in 2016 as an academic-government research partnership.

On the Effect of Age-Dependent Mortality on the Stability of a System of Delay-Differential Equations Modeling Erythropoiesis.

We present an age-structured model for erythropoiesis in which the mortality of mature cells is described empirically by a physiologically realistic probability distribution of survival times. Under some assumptions, the model can be transformed into a system of delay differential equations with both constant and distributed delays. The stability of the equilibrium of this system and possible Hopf bifurcations are described for a number of probability distributions.

Acceptability to nurses of reducing NICU light and noise levels during skin-to-skin care: A pilot study.

Introduction: Light and noise levels may influence preterm infants and their mothers when they are experiencing skin-to-skin contact [SSC] in the Neonatal Intensive Care Unit [NICU].

Methods: A pilot randomized controlled trial [RCT] of an intervention aiming at reducing light and noise levels during SSC was conducted. Twenty-one neonatal nurses from a level III NICU completed questionnaires assessing their acceptability of NICU light and noise levels reduction during SSC, whether it interfered with their care delivery, in addition to acceptability of specific interventions reducing these levels.

What explains cross-city variation in mortality during the 1918 influenza pandemic? Evidence from 438 U.S. cities.

Disparities in cross-city pandemic severity during the 1918 Influenza Pandemic remain poorly understood. This paper uses newly assembled historical data on annual mortality across 438 U.S.

Monolayer Arrays of Nanoparticles on Block Copolymer Brush Films.

Two-dimensional arrays of nanoparticles (NPs) have widespread applications in optical coatings, plasmonic sensors, and nanocomposites. Current bottom-up approaches that use homogeneous NP templates, such as silane self-assembled monolayers or homopolymers, are typically plagued by NP aggregation, whereas patterned block copolymer (BCP) films require specific compositions for specific NP distributions. Here, we show, using polystyrene- b-poly(4-vinylpyridine) (PS- b-P4VP) and gold NPs (AuNPs) of various sizes, that a nanothin PS- b-P4VP brushlike coating (comprised of a P4VP wetting layer and a PS overlayer), which is adsorbed onto flat substrates during their immersion in very dilute PS- b-P4VP tetrahydrofuran solutions, provides an excellent template for obtaining dense and well-dispersed AuNPs with little aggregation.

[The oncology care pathway and its transitions, an exploratory literature review].

A literature review was undertaken to describe the experience of people with cancer with regard to the different transitions of their care pathway. It enables all these transitions to be identified, documented and mapped out. The aim is to improve the quality of the care provided to this vulnerable population, all along the pathway.

Measured and perceived effects of audit and feedback on nursing performance: a mixed methods systematic review protocol.

Background: The use of audit and feedback (A&F) interventions in health care has been demonstrated to generally be effective on medical teams. However, literature suggests that the response of nurses to this type of intervention may differ from that of other types of health professionals, in relation to their roles, power, and to the configuration of nursing care activities. To our knowledge, no review has been conducted on A&F interventions with nurses.

Polymer-Templated Gold Nanoparticles on Optical Fibers for Enhanced-Sensitivity Localized Surface Plasmon Resonance Biosensors.

Dense arrays of well-dispersed gold nanoparticles (AuNPs) on optical fibers are shown to bridge the gap in sensitivity and sensing performance between localized surface plasmon resonance (LSPR) and classical SPR sensing. A simple self-assembly method relying on a poly(styrene- b-4-vinylpyridine) (PS- b-P4VP) block copolymer brush layer was used to immobilize AuNPs of different diameters from 10 to 92 nm on optical fibers. In comparison with standard AuNP deposition methods using (3-aminopropyl)trimethoxysilane (APTMS) and polyelectrolytes, the sensitivity with the PS- b-P4VP templating method was found to be 3-fold better, a consequence of the smaller gap between particles and the presence of fewer AuNP aggregates.

Nurses' practices regarding procedural pain management of preterm infants.

Background: It is well known that preterm neonates can feel pain which can be expressed through specific behaviors and signs. Repeated and untreated pain has consequences for the preterm neonates such as hypersensitivity to pain, as well as important repercussions on their motor and intellectual development. The use of non-pharmacological interventions for pain management by nurses is imperative to prevent these consequences in the NICU.