Discovery and Characterization of Four Aphelenchid Species from Cultivated Regions of Southern Alberta, Canada.

The nematode family Aphelenchoididiae is considered fungal-feeding, predatory, or root hair feeders. Some members of this family are universally present in agricultural landscapes and are an integral part of soil health and conservation studies. In the present soil nematode biodiversity survey, we detected four species of the genera , , and .

An anti-mitotic compound, (+)-6-tuliposide A, isolated from the Canadian glacier lily, Erythronium grandiflorum.

The Canadian prairie ecosystem is subjected to abiotic and biotic conditions that induce plants to produce secondary metabolites that affect mammalian physiology. Extracts prepared from certain plant species native to Canadian prairie and montane cordillera ecosystems have previously been shown to have anti-mitotic activity on human cancer cell lines. In this study, we investigated the glacier lily, Erythronium grandiflorum (Liliaceae), in which the species was the most phylogenetically distant from Asteraceae and had anti-mitotic activity.

Morpho-Anatomy, In Vitro Culture, and Phylogenetic Studies of Two Species from Southern Alberta, Canada.

Spiral nematodes ( spp.) are polyphagous parasitic species exhibiting a broad host range and geographical distribution. However, their diversity in the cultivated regions of southern Alberta remains understudied.

Heating and storage of structured acylglycerols with succinyl-linked stigmasterol residue does not cause negative chemical or biological changes.

Four structured acylglycerols with stigmasterol bonded by a succinyl linker were investigated and their stability were analyzed. Samples were heated to 60 °C and kept at that temperature to simulate storage, and to 180 °C to simulate frying conditions. The degradation of the synthesized compounds and formed derivatives was determined, and their cytotoxicity and genotoxicity on normal human cells from the digestive system was determined.

Validating a multi-locus metabarcoding approach for characterizing mixed-pollen samples.

Background: The mutualistic interaction between entomophilous plants and pollinators is fundamental to the structure of most terrestrial ecosystems. The sensitive nature of this relationship has been disrupted by anthropogenic modifications to natural landscapes, warranting development of new methods for exploring this trophic interaction. Characterizing the composition of pollen collected by pollinators, e.

Habitat and climate influence hybridization among three genetically distinct Canada jay (Perisoreus canadensis) morphotypes in an avian hybrid zone complex.

Examining the frequency and distribution of hybrids across contact zones provide insights into the factors mediating hybridization. In this study, we examined the effect of habitat and climate on hybridization patterns for three phenotypically, genetically, and ecologically distinct groups of the Canada jay (Perisoreus canadensis) in a secondary contact zone in western North America. Additionally, we tested whether the frequency of hybridization involving the three groups (referred to as Boreal, Pacific and Rocky Mountain morphotypes) is similar across the hybrid zones or whether some pairs have hybridized more frequently than others.

Are There Riskier Types of Gambling?

Gambling-related harm is a public health issue requiring market regulation and efforts aimed at prevention and treatment. An important consideration for the regulation of gambling is whether certain types of gambling are intrinsically more harmful than others. The present study was a comprehensive investigation of this issue in a nationwide sample of 10,199 Canadian adult gamblers that included 1346 individuals with problem gambling.

Thermo-oxidative stability and safety of new acylglycerols with stigmasterol residue: Effects of fatty acids saturation and position in the glycerol backbone.

The safety and thermoxidative stability of new diacyl-stigmasterylcarbonoyl-sn-glycerols (DAStGs) with two molecules of palmitic or oleic acids and one molecule of stigmasterol at the sn-2 or sn-3 position were studied. After heating to 60 °C, the compounds with stigmasterol at the sn-2 position were more stable than those with stigmasterol at the sn-3 position. The lowest level of degradation of stigmasterol after heating to 180 °C was detected for both compounds with oleic acid, followed by the samples with palmitic acid.

New Records of Species from Southern Alberta, Canada.

The presence of plant-parasitic nematodes (PPNs) in cultivated areas is a limiting factor in achieving marketable crop yield. To control and alleviate the effects of these nematodes and determine appropriate management strategies, species-level identification is crucial. Therefore, we conducted a nematode diversity survey, which resulted in the detection of four species in cultivated areas of southern Alberta, Canada.

Potential host range of (Hymenoptera: Braconidae), a parasitoid new to North America and a possible biological control agent of noctuid pest species.

The likelihood of parasitoids establishing in new geographic regions depends upon the availability of suitable host species. Identifying these hosts and the degree of their suitability is particularly important when they include species that are economically important as pests. In laboratory studies, we examined the suitability of 47 species of Lepidoptera as potential hosts of a parthenogenetic strain of the gregarious parasitoid (Hymenoptera: Braconidae).

Thermo-oxidative stability of asymmetric distigmasterol-modified acylglycerols as novel derivatives of plant sterols.

The study investigated the thermo-oxidative stability of distigmasterol-modified acylglycerols as a new structured acylglycerols. Samples were heated at 60 and 180 °C for 8 h. Their percentage degradation and products formed during heating were compared with free stigmasterol and stigmasteryl esters.

How trees thrive in a dry climate: diurnal and seasonal hydrology and water relations in a riparian cottonwood grove.

In semi-arid ecoregions, trees are restricted to river valley floodplains where river water supplements the limited precipitation. To characterize the associated diurnal and seasonal dynamics in hydrology and water relations, we studied narrowleaf cottonwoods (Populus angustifolia) along a prairie river in Canada. From June through August, the shallow soil moisture was depleted but moisture remained higher above the alluvial groundwater table, which dropped to 2.

Overview of the Genus and First Reports of and from Southern Alberta, Canada.

The present study provides the morphological and molecular characterization of and populations, recovered from agricultural fields of southern Alberta. Despite a significant abundance of this group of nematodes, none of the species were previously reported in Canada. Therefore, representative adult specimens of each population were photographed and examined morphometrically.

Integrative taxonomy, distribution, and host associations of and from southern Alberta, Canada.

Two stunt nematode species, and , were recovered from the potato growing regions of southern Alberta, described and characterized based on integrative taxonomy. Morphometrics, distribution, and host associations of both species are discussed. The Canadian populations of both species displayed minor variations in morphometrical characteristics (viz.

Species Diversity of Pin Nematodes ( spp.) from Potato Growing Regions of Southern Alberta, Canada.

Pin nematodes ( spp.) are polyphagous parasitic species with a wide host range and geographical distribution; their diversity is unknown in the potato growing region of Alberta, Canada. The present study aims to provide morphological and molecular characterization of three pin nematode species, namely , , and a new species, sp.

Changes in the recovery of insects in pitfall traps associated with the age of cow dung bait fresh or frozen at the time of placement.

Pitfall traps baited with cattle dung are commonly used to characterize local assemblages of coprophilous insects. Baits can be made fresh or be prepared in advance and kept frozen until needed. Insect recoveries are expected to decline with the age of the bait and may be affected by the use of fresh vs.

Reactions of Molybdenum and Tungsten Oxide Tetrafluoride with Sulfur(IV) Lewis Bases: Structure and Bonding in [WOF], MOF(OSO), and [SF][MOF] (M = Mo, W).

The structure of [WOF] has been reinvestigated by low-temperature X-ray crystallography and DFT (MN15/def2-SVPD) studies. Whereas the WF ring of the tetramer is planar and disordered in the solid state, the optimized gas-phase geometry prefers a disphenoidally puckered WF ring and demonstrates asymmetric fluorine bridging. Dissolution of MOF (M = Mo, W) in SO and SF results in the formation of MOF(OSO) and [SF][MOF], respectively.

Computational Insight into the Differential Mutagenic Patterns of O-Methylthymine Lesions.

Differential mutagenic patterns were recently reported for O-methylated thymine lesions, which indicate that O4-methylthymine (O4-Me-T) frequently leads to G misinsertions, whereas O2-methylthymine (O2-Me-T) is primarily nonmutagenic. The reasons for these differences are unclear since both lesions similarly alter the Watson-Crick binding face of T. To rationalize these replication outcomes at a molecular level, this work uses density functional theory calculations and molecular dynamics simulations to probe the lesion base-pairing properties as well as lesion accommodation by human polymerase η (pol η) and post-extension DNA duplexes.

An H-Substituted Rhodium Silylene.

Divergent reactivity of organometallic rhodium(I) complexes, which led to the isolation of neutral rhodium silylenes, is described. Addition of PhRSiH (R=H, Ph) to the rhodium cyclooctene complex ( NNN)Rh(COE) (1-COE; NNN=2,5-[iPr P=N(4-iPrC H )] N(C H ) , COE=cyclooctene) resulted in the oxidative addition of an Si-H bond, providing rhodium(III) silyl hydride complexes ( NNN)Rh(H)SiHRPh (R=H, 2-SiH Ph; Ph, 2-SiHPh ). When the carbonyl complex ( NNN)Rh(CO) (1-CO) was treated with hydrosilanes, base-stabilized rhodium(I) silylenes κ -N,N-( NNN)(CO)Rh=SiRPh (R=H, 3-SiHPh; Ph, 3-SiPh ) were isolated and characterized using multinuclear NMR spectroscopy and X-ray crystallography.

Cellular roles of the human Obg-like ATPase 1 (hOLA1) and its YchF homologs.

P-loop NTPases comprise one of the major superfamilies of nucleotide binding proteins, which mediate a variety of cellular processes, such as mRNA translation, signal transduction, cell motility, and growth regulation. In this review, we discuss the structure and function of two members of the ancient Obg-related family of P-loop GTPases: human Obg-like ATPase 1 (hOLA1), and its bacterial/plant homolog, YchF. After a brief discussion of nucleotide binding proteins in general and the classification of the Obg-related family in particular, we discuss the sequence and structural features of YchF and hOLA1.

Eukaryotic initiation factor 5B (eIF5B) provides a critical cell survival switch to glioblastoma cells via regulation of apoptosis.

Physiological stress conditions attenuate global mRNA translation via modifications of key eukaryotic initiation factors. However, non-canonical translation initiation mechanisms allow cap-independent translation of certain mRNAs. We have previously demonstrated that eIF5B promotes cap-independent translation of the mRNA encoding the antiapoptotic factor, XIAP, during cellular stress.

Eukaryotic Initiation Factor 5B (eIF5B) Cooperates with eIF1A and eIF5 to Facilitate uORF2-Mediated Repression of ATF4 Translation.

A variety of cellular stresses lead to global translation attenuation due to phosphorylation of the alpha subunit of eukaryotic initiation factor 2 (eIF2), which decreases the availability of the eIF2-GTP-Met-tRNA ternary complex. However, a subset of mRNAs continues to be translated by non-canonical mechanisms under these conditions. In fact, although translation initiation of activating transcription factor 4 (ATF4) is normally repressed by an upstream open reading frame (uORF), a decreased availability of ternary complex leads to increased translation of the main ATF4-coding ORF.

Competition among Strains for Space and Resources.

Shiga toxin-producing (STEC) are a subgroup of causing human diseases. Methods to control STEC in livestock and humans are limited. These and other emerging pathogens are a global concern and novel mitigation strategies are required.

Heterosis in poplar involves phenotypic stability: cottonwood hybrids outperform their parental species at suboptimal temperatures.

Heterosis or hybrid vigor is common in hybrid poplars, and to investigate its occurrence and physiological basis we compared narrowleaf cottonwoods, Populus angustifolia James, prairie cottonwoods, Populus deltoides Bartr. ex Marsh, and their native intersectional hybrids, P. × acuminata Rydb.