Inflammation alters the expression and activity of the mechanosensitive ion channels in periodontal ligament cells.

Background: Periodontal ligament cells (PDLCs) possess mechanotransduction capability, vital in orthodontic tooth movement (OTM) and maintaining periodontal homeostasis. The study aims to elucidate the expression profiles of mechanosensitive ion channel (MIC) families in PDLCs and how the inflammatory mediator alters their expression and function, advancing the understanding of the biological process of OTM.

Methods And Methods: Human PDLCs were cultured and exposed to TNF-α.

A self-attention-driven deep learning framework for inference of transcriptional gene regulatory networks.

The interactions between transcription factors (TFs) and the target genes could provide a basis for constructing gene regulatory networks (GRNs) for mechanistic understanding of various biological complex processes. From gene expression data, particularly single-cell transcriptomic data containing rich cell-to-cell variations, it is highly desirable to infer TF-gene interactions (TGIs) using deep learning technologies. Numerous models or software including deep learning-based algorithms have been designed to identify transcriptional regulatory relationships between TFs and the downstream genes.

Gelatin Methacryloyl/Sodium Alginate/Cellulose Nanocrystal Inks and 3D Printing for Dental Tissue Engineering Applications.

In tissue engineering, developing suitable printing inks for fabricating hydrogel scaffolds via 3D printing is of high importance and requires extensive investigation. Currently, gelatin methacryloyl (GelMA)-based inks have been widely used for the construction of 3D-printed hydrogel scaffolds and cell-scaffold constructs for human tissue regeneration. However, many studies have shown that GelMA inks at low polymer concentrations had poor printability, and printed structures exhibited inadequate fidelity.

Advantages and feasibility of prefabricated PEEK crowns for aesthetic restoration in primary teeth.

Objectives The main purpose of this study is to evaluate the efficacy of polyetheretherketone (PEEK) as a material for prefabricated crown restorations in pediatric dentistry, particularly for restoring primary tooth structure defects. Materials and methods This study analyzed the effects of three surface treatment modalities on PEEK's surface morphology, wettability (as measured by contact angle), and shear bond strength. These treatments included alumina (AlO) sandblasting alone, and in conjunction with a 98% sulfuric acid pretreatment.

Perception of facial esthetics and cephalometric correlations in Class II patients: a comparison between two-phase and one-phase treatments.

An effective orthodontic treatment should not only aim for satisfactory occlusal outcomes but also consider its impact on facial esthetics. The study aims to evaluate and compare the perception of profile esthetics of skeletal Class II patients treated with two orthodontic modalities: (1) Two-phase approach involving functional appliances followed by fixed appliances with premolar extractions, or (2) One-phase approach using fixed appliances with premolar extractions. Additionally, the study aims to evaluate the correlation between the perceived esthetics and the corresponding cephalometric measurements.

Alterations in salivary profile in individuals with dental caries and/or obesity: A systematic review and meta-analysis.

Objectives: To investigate current evidence on the association of salivary profile with dental caries and obesity.

Data/sources: A comprehensive search was conducted on PubMed, Web of Science, Embase, ProQuest Medical Database, ProQuest Sci-Tech Premium, and Cochrane Library up to June 2024. Observational studies comparing salivary profiles in individuals with dental caries and/or obesity were included.

3D printed gelatin/PTMC core/shell scaffolds with NIR laser-tuned drug/biomolecule release for cancer therapy and uterine regeneration.

Surgical resection is an efficient treatment for cancerous tissues and uterine fibroids in the women uterus. However, the insufficiency of clinical interventions could result in tumor recurrence, and the defective tissues remained would cause intrauterine adhesions (IUAs) and further affect reproduction capacity. In this study, 3D printed hydrogel/poly(l-lactide-co-trimethylene carbonate) (PLLA-co-TMC, "PTMC" in short) core/shell scaffolds with NIR-tuned doxorubicin hydrochloride (DOX) and estradiol (E2) dual release were designed and fabricated for cancer therapy and uterine regeneration.

Glass ionomer fissure sealants versus fluoride varnish application on children's behaviour: a randomised controlled trial.

Purpose: Glass ionomer fissure sealant (GIS) and fluoride varnish (NaFV) are two preventive interventions applicable to pre-school children. However, their application effects on young children's behaviour are understudied. The study compared the effects of GIS and NaFV applications on dental anxiety (DA), cooperativeness and pain level in pre-school children when applied to primary second molars in outreach settings.

Constructing the Enamel-Like Dentin Adhesion Interface to Achieve Durable Resin-Dentin Adhesion.

Enamel adhesion is acknowledged as durable; however, achieving long-lasting dentin adhesion remains a formidable challenge due to degradation of exposed collagen matrix after acid-etching of dentin. The idea of developing an enamel-like adhesion interface holds great promise in achieving enduring dentin adhesion. In this study, we constructed an enamel-like adhesion interface using a rapid remineralization strategy comprising an acidic primer and a rapid remineralization medium.

Enhancing craniofacial bone tissue engineering strategy: integrating rapid wet chemically synthesised bioactive glass with photopolymerized resins.

Background: Craniofacial bone regeneration represents a dynamic area within tissue engineering and regenerative medicine. Central to this field, is the continual exploration of new methodologies for template fabrication, leveraging established bio ceramic materials, with the objective of restoring bone integrity and facilitating successful implant placements.

Methods: Photopolymerized templates were prepared using three distinct bio ceramic materials, specifically a wet chemically synthesized bioactive glass and two commercially sourced hydroxyapatite variants.

Preventing dentin erosion with silver diamine fluoride and salivary pellicle: an in vitro study.

Objectives: To investigate the preventive and discoloring effects of a single and two weekly applications of 38% silver diamine fluoride (SDF) against dentin erosion.

Materials And Methods: 180 dentin blocks were divided into four groups. Group 1 (SDF2) received two weekly applications of 38% SDF.

Human-AI collaboration improves adults' oral biomechanical functions: A multi-centre, self-controlled clinical trial.

Objectives: Maintenance of oral muscle functions is important for survival and communication. Utilizing Artificial Intelligence (AI) as a self-health-management material has shown promise. Here we developed a functional and AI-enabled smartphone e-Oral APP that provided real-time feedback features to let humans collaborate with AI, targeting to improve oral biomechanical and speech functions of adults.

Preventing proximal enamel caries in neighboring tooth with glass ionomer cement restoration and silver diamine fluoride pretreatment.

Objective: To investigate caries preventive effects of 38 % silver diamine fluoride (SDF) pretreatment on neighboring tooth proximal to glass ionomer cement (GIC), including conventional GIC (CGIC) and resin-modified GIC (RMGIC) restorations in an in vitro model.

Methods: HUMAN TOOTH BLOCKS WERE RESTORED WITH: SDF+CGIC (Group 1), CGIC (Group 2), SDF+RMGIC (Group 3) or RMGIC (Group 4). Enamel specimen simulating proximal surface of neighboring tooth was placed in proximity to the restorations.

The Anterolateral Thigh Flap in Head and Neck Reconstruction.

The anterolateral thigh (ALT) free flap has become a workhorse for head and neck reconstruction. This paper offers a thorough introduction to the ALT flap, covering its anatomy, surgical technique, adaptable designs, and use in a range of clinical settings along with case studies. With its long vascular pedicle and tissue versatility, the ALT flap is well-suited for matching varied defects.

Randomized clinical trial to compare three fluoride varnishes in preventing early childhood caries.

Objectives: To compare the effectiveness of two 5% sodium fluoride (NaF) varnishes containing casein phosphopeptide amorphous calcium phosphate (CPP-ACP) (MI Varnish) or tricalcium phosphate (TCP) (Clinpro White) to the conventional 5% NaF varnish (Duraphat®) in preventing early childhood caries (ECC) in high-risk preschool children.

Methods: A double-blinded, randomized controlled trial recruited healthy 3-4-year-old children (N = 582) having at least one carious lesion (pre-cavitated or cavitated) after obtaining written informed consent from parents. Using a computer-generated random-number table, children were assigned to one of the 3 groups: Control group (n = 196): 5% NaF varnish (Duraphat®) or two test groups: 5% NaF with TCP (Clinpro™ White) (n = 193) and 5% NaF varnish with CPP-ACP (MI Varnish™) (n = 193) to receive quarterly (every 3 months) application over 24 months.

METTL3-mediated pre-miR-665/DLX3 mA methylation facilitates the committed differentiation of stem cells from apical papilla.

Methyltransferase-like 3 (METTL3) is a crucial element of N6-methyladenosine (mA) modifications and has been extensively studied for its involvement in diverse biological and pathological processes. In this study, we explored how METTL3 affects the differentiation of stem cells from the apical papilla (SCAPs) into odonto/osteoblastic lineages through gain- and loss-of-function experiments. The mA modification levels were assessed using mA dot blot and activity quantification experiments.

Extrafibrillar demineralization: Yes or no?

Objective: Extrafibrillar demineralization is considered to be an ideal solution for addressing the durability of resin-dentin bonding interfaces. However, its theoretical basis is contradictory to ionization equilibrium of hydroxyapatite dissolution. In this study, various calcium chelators were selected as dentin conditioners to explore the essence of dentin demineralization with chelators and its effect on resin-dentin adhesion.

Clinical treatment patterns, molecular characteristics and survival outcomes of ROS1-rearranged non-small cell lung cancer: A large multicenter retrospective study.

Background: Non-small cell lung cancer (NSCLC) harboring ROS1 rearrangements is a molecular subset that exhibits favorable responses to tyrosine kinase inhibitor (TKI) treatment than chemotherapy. This study investigated real-world treatment patterns and survival outcomes among patients with ROS1-rearranged advanced NSCLC.

Methods: We conducted a retrospective analysis of patients with ROS1-rearranged advanced NSCLC treated in four different hospitals in China from August 2018 to March 2022.

Bottom-Up Assembling Hierarchical Enamel-Like Bulk Materials with Excellent Optical and Mechanical Properties for Tooth Restoration.

Enamel has good optical and mechanical properties because of its multiscale hierarchical structure. Biomimetic construction of enamel-like 3D bulk materials at nano-, micro-, mesh- and macro-levels is a challenge. A novel facile, cost-effective, and easy large-scale bottom-up assembly strategy to align 1D hydroxyapatite (HA) nanowires bundles to 3D hierarchical enamel structure with the nanowires bundles layer-by-layer interweaving orientation, is reported.

Biomechanical Considerations in the Orthodontic Treatment of a Patient with Stabilised Stage IV Grade C Generalised Periodontitis: A Case Report.

Orthodontic treatment of periodontally compromised patients presents unique challenges, including controlling periodontal inflammation, applying appropriate force, designing an effective dental anchorage, and maintaining treatment results. Deteriorated periodontal support leads to alterations in the biological responses of teeth to mechanical forces, and thus orthodontists must take greater care when treating patients with periodontal conditions than when treating those with a good periodontal status. In this article, we report the case of a 59-year-old woman with stabilised Stage IV grade C generalised periodontitis characterised by pathological tooth migration (PTM).

Preventive dental erosion with silver diamine fluoride: An in vitro study.

Objectives: To evaluate the erosion preventive effect of 38 % silver diamine fluoride (SDF) solution in enamel and dentin of human permanent teeth.

Methods: Ninety enamel and ninety dentin blocks were prepared from permanent molars and allocated into three groups. Gp-SDF received a one-off application of 38 % SDF solution.