[Cellular phenotype conversion induced by co-culture of human mesenchymal stem cells cocultured with human sweat gland cells].

Objective: To investigate the cellular phenotype conversion during human mesenchymal stem cells (hMSCs) cocultured with injured human sweat gland cells (hSGCs) in vitro.

Methods: HMSCs and hSGCs were isolated and cultured and expanded respectively. The antigens expression of hMSCs and hSGCs were detected by two-steps immunocytochemistry.

[Gene expression of C2 subunit of proteasome in cardiac muscle in burned rats with sepsis].

Objective: To study the gene expression of C2 subunit of proteasome and proteolysis in cardiac muscle in burned animals with sepsis.

Methods: Male Wistar rats were randomly divided into burn group, burn sepsis group and control group. The extent of burn injury was 30% total body surface area (TBSA) III degree burn, and the model of burn sepsis was replicated by administration of endotoxin (6 mg/kg) into the peritoneal cavity immediately after burn injury.

[Effects of carbachol on apoptosis of peripheral white blood cells and expression of cytokines in rats suffering from gut ischemia/reperfusion injury].

Objective: To evaluate the effect of cholinergic drug carbachol on apoptosis and expression of certain cytokines of peripheral blood lymphocytes and neutrophils in rats subjected to ischemia/reperfusion injury of the intestine.

Methods: Thirty-six Wistar male rats were randomly divided into groups as follows: sham operation, 1 and 2 hour-gut ischemia, 1 hour-gut ischemia followed by reperfusion for 1 hour and 2 hours, carbachol + 1 hour-gut ischemia, and carbachol + 1 hour-gut ischemia followed by reperfusion for 2 hours. Then the gut was subjected to ischemia/reperfusion.

[Protective effects of acidic fibroblast growth factor on intestinal ischemia/reperfusion in rats].

Objective: To observe the change in hepatic and renal functions, change in the plasma D-lactate level, and the expression of proliferating cell nuclear antigen (PCNA) after intestinal I/R injury, so as to explore the effects of reconstructive human acid fibroblast growth factor(aFGF) on intestinal I/R injury in rats.

Methods: One hundred and twenty-six Wistar rats were divided into sham-operated, ischemia (45 minutes) plus reperfusion, reconstructive human aFGF treatment (2, 4, 8 microg aFGF) and wild type aFGF(2, 6, 12, and 24 hours, respectively) groups. Hepatic and renal functions and the levels of plasma D-lactate were determined and the expression of PCNA was assessed.

[Acellular porcine dermal matrix produced with different methods and an experimental study on its transplantation to skin wound].

Objective: To observed the effect of healing quality of composite skin grafting consisting of acellular porcine dermal matrix combined with autologous split-thickness skin graft.

Methods: Porcine skin was treated with dispase II/triton X-100 or hyperosmotic saline/sodium-dodecyl-sulfate (SDS) respectively, and acellular porcine dermal matrix I (APDMI) and APDM II were obtained. Sixty-three Sprague-Dawley rats with full-thickness skin defects on the back were separately covered with APDMT + split-thickness autologous skin, or APDM II + split-thickness autologous skin.

[Culture and identification of human skin fibroblasts].

Objective: To explore the method of isolation, cultivation, and identification of human skin fibroblasts in vitro.

Methods: By digesting human skin with collagenase type II to isolate human eccrine sweat glands. The fibroblasts grew along with the growth of eccrine sweat gland cells,and they were separated by digesting with 0.

[Effects of adipose tissue extract on rat skin regeneration in vitro].

Objective: To investigate the effects of adipose tissue extract on growth of rat skin in vitro.

Methods: Neonatal rat skin (area of 2 mm x 2 mm) was cultured in Dulbecco's modified Eagle's medium (DMEM) containing 10% adipose tissue extract and 10% phosphate buffered solution (PBS). After being cultured for 6 days, the area of skin was measured.

[Enteral administration of carbachol on systemic inflammatory response and multiple organ dysfunction induced by partial ischemia/reperfusion injury to the gut].

Objective: To study the effects of enteral administration of carbachol on organ dysfunction induced by partial ischemia/reperfusion injury to the intestine.

Methods: Seventy-five white rabbits were randomized into four groups: ischemia/reperfusion (I/R), carbachol+ I/R and sham operation. The superior mesenteric artery (SMA) was partially blocked with self-designed blocker, producing 50% decrease in SMA blood flow, lasting for 4 hours.

[Protective effects of modified recombinant human acidic fibroblast growth factor on small intestine after ischemia/reperfusion injury in rats].

Objective: To explore the protective effect of modified recombinant human acidic fibroblast growth factor (rhaFGF) on small intestinal after ischemia/reperfusion (I/R) injury in rats.

Methods: The clamp on the superior mesenteric artery (SMA) was removed after clamping it for 45 minutes to replicate I/R injury of the intestine in the rat. Rats were then divided randomly into sham operation group, normal saline treatment group and rhaFGF treatment group, in which the rats of the normal saline treatment group were injected 0.

[Comparison of microinjection methods for subretinal RPE transplantation in the rat].

Objective: To compare iatrogenic damage to the host eye caused by different injection techniques for transplantation RPE into the subretinal space of Lewis rats.

Methods: A total of 45 eyes from Lewis rats were divided into three groups and immortalized hTERT-RPE1 cells (clontechniques) transfected with green fluorescent protein (GFP) was injected into the subretinal space. The injection was given by using Hamilton syringe, Eppendorf repeat micro-pipettor, or a pediatric syringe infusion pump in different groups respectively.