Changes of maternal serum natural antibodies of IgG class to proteins MBP, S100, ACBP14/18 and MP65 and embryonic misdevelopments in humans.

Immunoreactivity that is contents/affinity of natural antibodies (N-Ab) of IgG class to developmentally related proteins MBP, S100, ACBP14/18 and MP65 were measured in serum samples of 1987 women at their pregnancy terms of 3-12 weeks. The pregnancy results of the examined women were analyzed 4-9 months later. It was revealed that frequencies of cases of stopped early pregnancies, intrauterine and antenatal fetal deaths, and births of newborns with coarse inborn defects are directly related to deviation of the examined maternal N-Ab content/affinity from the physiological limits: abnormally high as well as abnormally low reactivity during first trimester of pregnancy, both could be related to unfavorable results of pregnancy.

Possible mechanisms of diabetic fetopathy.

By using immunoaffine chromatography and ELISA it was found that 30-35% polyclonal of anti-insulin antibodies (AB1) in sera of diabetes patients has bound with high-affinity to Nerve Growth Factor (NGF). This indicates the presence of common epitop(s) in both molecules and could be a reason for NGF deprivation during formation of the nervous system in fetuses of diabetic pregnant women. Patient sera also usually characterized by elevated level of antiidiotypic antibodies (AIAb2) which interact with membrane insulin receptors, and may induce the general metabolic disturbances in fetus and newborns from diabetic mothers, fatal sometimes.