Effect of liquid-handling accuracy on assay performance.

This study illustrates how optimization of both liquid-handling accuracy and precision is critical to assay performance. The study was designed to examine (1) liquid-handling performance and (2) the effect of liquid-handling variability on two types of in vitro biochemical assays by making small but deliberate changes to assay volume delivery. Specifically, protein binding (streptavidin) and enzyme (α-galactosidase) assays were investigated by determining the effect of assay volume for each assay component.