A rapid and sensitive enzymatic assay for 2,3-butanediol.

In this study, we developed a rapid and sensitive enzymatic assay for 2,3-butanediol (2,3-BDO) detection. The concentration of 2,3-BDO was determined by measuring the reduction of NADP using 2,3-butanediol dehydrogenase (CL-Bdh). The enzymatic assay could detect as low as 0.

Biohydrogen production of obligate anaerobic archaeon NA1 under oxic conditions via overexpression of -encoding hydrogenase genes.

Background: The production of biohydrogen (H) as a promising future fuel in anaerobic hyperthermophiles has attracted great attention because H formation is more thermodynamically feasible at elevated temperatures and fewer undesired side products are produced. However, these microbes require anoxic culture conditions for growth and H production, thereby necessitating costly and time-consuming physical or chemical methods to remove molecular oxygen (O). Therefore, the development of an O-tolerant strain would be useful for industrial applications.