Diagnostic potential of multimodal imaging of ovarian tissue using optical coherence tomography and second-harmonic generation microscopy.

Ovarian cancer is particularly deadly because it is usually diagnosed after it has metastasized. We have previously identified features of ovarian cancer using optical coherence tomography (OCT) and second-harmonic generation (SHG) microscopy (targeting collagen). OCT provides an image of the ovarian microstructure while SHG provides a high-resolution map of collagen fiber bundle arrangement.

Hyper-numerical aperture (NA = 2.8) microscope using λ = 1.56 µm femtosecond source for multi-photon imaging.

A new microscope is discussed, where the scanning illumination has a numerical aperture of 2.8 with λ = 1.56 µm femtosecond fiber laser.