Exploring the Use of Water-Extracted Flaxseed Hydrocolloids in Three-Dimensional Cell Culture.

Plant-derived hydrocolloids offer promising prospects in biomedical applications. Among these, Flaxseed hydrocolloid (FSH) can form a soft, elastic, and biocompatible hydrocolloid with tunable viscosity and superior swelling capacity, making it an attractive scaffold. This study introduces a green extraction method for FSH, employing a single-step aqueous extraction process and fabrication of FSH scaffold.

Arabinoxylan-based psyllium seed hydrocolloid: Single-step aqueous extraction and use in tissue engineering.

Biomacromolecules derived from natural sources offer superior biocompatibility, biodegradability, and water-holding capacity, which make them promising scaffolds for tissue engineering. Psyllium seed has gained attention in biomedical applications recently due to its gel-forming ability, which is provided by its polysaccharide-rich content consisting mostly of arabinoxylan. This study focuses on the extraction and gelation of Psyllium seed hydrocolloid (PSH) in a single-step water-based protocol, and scaffold fabrication using freeze-drying method.

"He is just Ken:" deconstructing hegemonic masculinity in Barbie (2023 Movie).

Scholars have consistently explored Barbie in various contexts, often subjecting it to critical analysis. However, the release of the Barbie 2023 Movie has shifted our focus from Barbie to Ken, marking the first occasion when Barbie has provided a platform for exploring representations of masculinity both in the patriarchal society and in popular culture. This article aims to investigate how the 2023 Barbie movie deconstructs symbols of hegemonic and toxic masculinity and its performative aspects within the framework of (post)feminist discourse.

Exploring Neuronal Differentiation Profiles in SH-SY5Y Cells through Magnetic Levitation Analysis.

Magnetic levitation (MagLev) is a powerful and versatile technique that can sort objects based on their density differences. This paper reports the sorting of SH-SY5Y cells for neuronal differentiation by the MagLev technique. Herein, SH-SY5Y cells were differentiated with retinoic acid (RA) and brain-derived neurotrophic factor (BDNF).

Biopatterning of 3D Cellular Model by Contactless Magnetic Manipulation for Cardiotoxicity Screening.

Impact statement Contactless manipulation and cell patterning techniques provide rapid and cost-effective three-dimensional (3D) cell culture model formation for tissue engineering applications. The present study introduces a new methodology that comprised alginate-based bioink to pattern cells via contactless magnetic manipulation to fabricate 3D cardiac structures. The developed cardiac model was evaluated in terms of Doxorubicin-induced cardiotoxicity and biopatterned 3D cardiac structures were found more resistant to drug exposure compared to two-dimensional control.

Fabrication and development of a microfluidic paper-based immunosorbent assay platform (μPISA) for colorimetric detection of hepatitis C.

Paper-based microfluidics is an emerging analysis tool used in various applications, especially in point-of-care (PoC) diagnostic applications, due to its advantages over other types of microfluidic devices in terms of simplicity in both production and operation, cost-effectiveness, rapid response time, low sample consumption, biocompatibility, and ease of disposal. Recently, various techniques have been developed and utilized for the fabrication of paper-based microfluidics, such as photolithography, micro-embossing, wax and PDMS printing, . In this study, we offer a fabrication methodology for a microfluidic paper-based immunosorbent assay (μPISA) platform and the detection of Hepatitis C Virus (HCV) was carried out to validate this platform.

Development of a hydrocolloid bio-ink for 3D bioprinting.

A new generation of bio-inks that are soft, viscous enough, stable in cell culture, and printable at low printing pressures is required in the current state of 3D bioprinting technology. Hydrogels can meet these features and can mimic the microenvironment of soft tissues easily. Hydrocolloids are a group of hydrogels which have a suitable gelling capacity and rheological properties.

Simultaneous validation of wearable motion capture system for lower body applications: over single plane range of motion (ROM) and gait activities.

Extracting data from {Zhu, 2019 #5} daily life activities is important in biomechanical applications to define exact boundary conditions for the intended use-based applications. Although optoelectronic camera-marker based systems are used as gold standard tools for medical applications, due to line-of-sight problem, there is a need for wearable, affordable motion capture (MOCAP) systems. We investigate the potential use of a wearable inertial measurement unit (IMU) based-wearable MOCAP system for biomechanical applications.

Fabrication of Tunable 3D Cellular Structures in High Volume Using Magnetic Levitation Guided Assembly.

Tunable and reproducible size with high circularity is an important limitation to obtain three-dimensional (3D) cellular structures and spheroids in scaffold free tissue engineering approaches. Here, we present a facile methodology based on magnetic levitation (MagLev) to fabricate 3D cellular structures rapidly and easily in high-volume and low magnetic field. In this study, 3D cellular structures were fabricated using magnetic levitation directed assembly where cells are suspended and self-assembled by contactless magnetic manipulation in the presence of a paramagnetic agent.

Glucuronoxylan-based quince seed hydrogel: A promising scaffold for tissue engineering applications.

Natural gums and mucilages from plant-derived polysaccharides are potential candidates for a tissue-engineering scaffold by their ability of gelation and biocompatibility. Herein, we utilized Glucuronoxylan-based quince seed hydrogel (QSH) as a scaffold for tissue engineering applications. Optimization of QSH gelation was conducted by varying QSH and crosslinker glutaraldehyde (GTA) concentrations.

Smartphone-assisted Hepatitis C detection assay based on magnetic levitation.

This work describes development of smartphone-assisted magnetic levitation assay for Point-of-Care (PoC) applications. Magnetic levitation is a technique that detects and separates particles based on their density differences in a magnetic field. Observation of the levitated micro-particles is mainly performed by light microscope or additional optical components, which mostly limits applicability of the magnetic levitation technique for PoC diagnostics.

Biomimetic hybrid scaffold consisting of co-electrospun collagen and PLLCL for 3D cell culture.

Electrospun collagen is commonly used as a scaffold in tissue engineering applications since it mimics the content and morphology of native extracellular matrix (ECM) well. This report describes "toxic solvent free" fabrication of electrospun hybrid scaffold consisting of Collagen (Col) and Poly(l-lactide-co-ε-caprolactone) (PLLCL) for three-dimensional (3D) cell culture. Biomimetic hybrid scaffold was fabricated via co-spinning approach where simultaneous electrospinning of PLLCL and Collagen was mediated by polymer sacrificing agent Polyvinylpyrrolidone (PVP).

Fluorescein propiolate: a propiolate-decorated fluorescent probe with remarkable selectivity towards cysteine.

A fluorescent probe decorated with an alkynyl ester unit (e.g. propiolate) displayed a selective turn-on type fluorescent response towards cysteine.

Correction: Scaffold-free three-dimensional cell culturing using magnetic levitation.

Correction for 'Scaffold-free three-dimensional cell culturing using magnetic levitation' by Esra Türker et al., Biomater. Sci.

Scaffold-free three-dimensional cell culturing using magnetic levitation.

Three-dimensional (3D) cell culture has emerged as a pioneering methodology and is increasingly utilized for tissue engineering, 3D bioprinting, cancer model studies and drug development studies. The 3D cell culture methodology provides artificial and functional cellular constructs serving as a modular playground prior to animal model studies, which saves substantial efforts, time and experimental costs. The major drawback of current 3D cell culture methods is their dependency on biocompatible scaffolds, which often require tedious syntheses and fabrication steps.

Recent Advances in Magnetic Levitation: A Biological Approach from Diagnostics to Tissue Engineering.

The magnetic levitation technique has been utilized to orientate and manipulate objects both in two dimensions (2D) and three dimensions (3D) to form complex structures by combining various types of materials. Magnetic manipulation holds great promise for several applications such as self-assembly of soft substances and biological building blocks, manipulated tissue engineering, as well as cell or biological molecule sorting for diagnostic purposes. Recent studies are proving the potential of magnetic levitation as an emerging tool in biotechnology.

A rhodamine/BODIPY-based fluorescent probe for the differential detection of Hg(II) and Au(III).

We described the design and synthesis of a molecular sensor based on a rhodamine/BODIPY platform that displayed differential fluorescence responses towards Hg(2+) and Au(3+) and demonstrated its utility in intracellular ion imaging.

A BODIPY aldoxime-based chemodosimeter for highly selective and rapid detection of hypochlorous acid.

A BODIPY-based fluorescent probe integrated with an aldoxime unit shows a remarkable fluorescence ''turn-on'' response to hypochlorous acid (HOCl). The oxidative dehydrogenation of BODIPY aldoxime by HOCl results in a distinct fluorescence enhancement as well as a change in color from red to orange.

A rhodamine based "turn-on" chemodosimeter for monitoring gold ions in synthetic samples and living cells.

A rhodamine-based fluorescent probe, functionalized with an alkyne moiety, shows highly selective recognition towards Au(i) and Au(iii) ions over other metal ions. Probe is successfully employed for the determination of residual gold species in synthetic samples and monitoring the accumulation of gold ions in living cells.