RBM15-dependent m6A modification mediates progression of non-small cell lung cancer cells.

Background: Non-small cell lung cancer (NSCLC) is the predominant form of lung cancer, contributing significantly to global health and economic challenges. This study elucidated the role of RBM15 in NSCLC progression through its involvement in m6A modifications.

Methods: RBM15 levels in NSCLC tissues and cells were assessed via RT-qPCR and Western blotting.

Safety, Pharmacokinetics and Antiviral Efficacy of Capsid Assembly Modulator Freethiadine in Healthy Volunteers and Chronic Hepatitis B Patients.

Background And Aims: Freethiadine is a novel hepatitis B virus capsid assembly modulator. Herein, we report the safety, tolerability, pharmacokinetics and 28-day antiviral activities of freethiadine.

Methods: The study consisted of two parts.

A general synthesis method for small-size and water-soluble NaYF:Yb, Ln upconversion nanoparticles at high temperature.

In this work, we developed a general synthesis method for the water-soluble lanthanide ion-doped NaYF upconversion (UC) nanoparticles (NPs) using triethylene glycol (TEG) as a high temperature solvent and diacid as a surfactant. Since the boiling point of the TEG is as high as 289.4 °C, the synthesis temperature can be correspondingly increased to a higher temperature that is a bit lower than this one.

Functionalized Cyclodextrin/Carboxymethyl Cellulose Composite Hydrogel with Double Network Structure for Adsorption of Heavy Metal Ions in Wastewater.

Heavy metal ions in industrial wastewater pose significant environmental and ecological threats. In this work, a hydrogel featuring a double network structure was synthesized via radical polymerization and cross-linking of β-cyclodextrin (CD) and carboxymethylcellulose (CMC) with acrylic acid (AA). The hydrogel's functional groups and microstructure were characterized using Fourier transform infrared spectroscopy (FTIR-ATR), X-ray diffraction (XRD), scanning electron microscopy (SEM), and thermogravimetric analysis (TGA).

LncRNA AFAP1-AS1 exhibits oncogenic characteristics and promotes gemcitabine-resistance of cervical cancer cells through miR-7-5p/EGFR axis.

Background: Drug resistance is the main factor contributing to cancer recurrence and poor prognosis. Exploration of drug resistance-related mechanisms and effective therapeutic targets are the aim of molecular targeted therapy. In our study, the role of long non-coding RNA (lncRNA) AFAP1-AS1 in gemcitabine resistance and related mechanisms were explored in cervical cancer cells.

A combined deep learning framework for mammalian m6A site prediction.

N-methyladenosine (m6A) is the most prevalent chemical modification in eukaryotic mRNAs and plays key roles in diverse cellular processes. Precise localization of m6A sites is thus critical for characterizing the functional roles of m6A in various conditions and dissecting the mechanisms governing its deposition. Here, we design a combined framework of Transformer architecture and recurrent neural network, deepSRAMP, to identify m6A sites using sequence-based and genome-derived features.

Structural characterization and anti-obesity effect of a novel water-soluble galactomannan isolated from Eurotium cristatum.

Obesity is a serious public health challenge worldwide, the present study is aimed to investigate the structural characteristic and anti-obesity effect of a water-soluble galactomannan (PEC) extracted from Eurotium cristatum (E. cristatum). Detailed analysis of the PEC structure showed a weight-average molecular weight of 32,305 Da and a composition of mainly mannose, galactose and small amounts of glucose.

Carboxymethyl chitosan-enhanced multi-level microstructured composite hydrogel scaffolds for bone defect repair.

Critical-sized bone defects (CSBDs) necessitate interventions like bone grafts or tissue engineering scaffolds to surpass the body's limited spontaneous healing capacity and ensure effective bone regeneration. A multi-level microstructured composite hydrogel 3D scaffold was fabricated for enhanced bone defect repair, integrating a 3D-printed macroporous polylactic acid (PLA) scaffold with polydopamine treatment and filled with a sodium alginate/nano hydroxyapatite/carboxymethyl chitosan (SA/nHA/CMCS) micrometer-scale porous composite hydrogel. The incorporation of nano hydroxyapatite (nHA) nanoparticles enhanced hydrogel crosslinking and osteogenic activity.

1,3-Dichloro-2-propanol Induced Renal Cell Ferroptosis via the Circadian Clock Protein BMAL1 Targeting GPX4.

1,3-Dichloro-2-propanol (1,3-DCP), a representative chloropropyl alcohol contaminant in food, has shown toxic effects on the kidney. Ferroptosis is a newly identified cell death driven by iron-dependent lipid peroxidation that is associated with renal injury. However, the role of 1,3-DCP in ferroptosis in renal cells remains unclear.

Saikosaponin inhibits Eimeria tenella infection by modifying the NF-κB pathway and regulating cytokines and the intestinal microbial community.

Eimeria tenella (E. tenella) is an intestinal parasite that not only endangers the health of broiler chickens but may also cause death in severe cases. However, the growing critical problem of drug resistance in E.

[Safety profile of tenofovir amibufenamide therapy extension or switching in patients with chronic hepatitis B: a phase Ⅲ multicenter, randomized controlled trial].

In chronic hepatitis B (CHB) patients with previous 96-week treatment with tenofovir amibufenamide (TMF) or tenofovir disoproxil fumarate (TDF), we investigated the safety profile of sequential TMF treatment from 96 to 144 weeks. Enrolled subjects that previously assigned (2:1) to receive either 25 mg TMF or 300 mg TDF with matching placebo for 96 weeks received extending or switching TMF treatment for 48 weeks. Safety profiles of kidney, bone, metabolism, body weight, and others were evaluated.

pH-sensitive metal-organic framework carrier decorated with chitosan for controlled drug release.

Satisfactory chemotherapeutic efficiency depends on improving the insufficient selectivity of chemotherapeutic drugs and decreasing damages to healthy tissues/cells, hence, the reasonable design and construction of drug carriers are of great significance in tumor treatment. Herein, the pH-sensitive MOF carrier was developed via the decoration of 4-formylphenylboronic acid (4-FPBA) for controlled 5'-deoxy-5-fluorocytidine (DFCR) release with selectivity. Besides, chitosan (CS) was introduced as the "gatekeeper" for the prevention of drug leakage.

Simultaneous Determination of Methotrexate and 7-Hydroxy-Methotrexate by UHPLC-MS Assay Coupled With Multiple Stage Fragmentation to Enhance Sensitivity.

A selective ultra-high performance liquid chromatography tandem mass spectrometry cubed (UHPLC/MS) assay for simultaneous determination of methotrexate (MTX) and 7-hydroxy-methotrexate (7-OH-MTX) in human plasma was developed and validated. After protein precipitation with methanol, chromatographic separation of MTX, MTX-d, and 7-OH-MTX was performed on a Waters AcQuity UPLC-BEH column (2.1 × 50 mm I.

Compromised macrophages contribute to progression of MASH to hepatocellular carcinoma in FGF21KO mice.

Metabolic dysfunction-associated steatohepatitis is well accepted as a potential precursor of hepatocellular carcinoma. Previously, we reported that fibroblast growth factor 21 (FGF21) revealed a novel anti-inflammatory activity via inhibiting the TLR4-IL-17A signaling, which could be a potential anticarcinogenetic mechanism to prevent to MASH-HCC transition. Here, we set out to determine whether FGF21 has a major impact on Kupffer cells' (KCs) ability during MASH-HCC transition.

Reactive Oxygen Species-Responsive Pillararene-Embedded Covalent Organic Frameworks with Amplified Antimicrobial Photodynamic Therapy for the Targeted Elimination of Periodontitis Pathogens.

Reactive oxygen species (ROS)-responsive drug delivery systems possess immense potential for targeted delivery and controlled release of therapeutics. However, the rapid responsiveness to ROS and sustained release of antibacterial drugs are often limited by the challenging microenvironment of periodontitis. Integrating ROS-responsive drug delivery systems with photocatalytic technologies presents a strategic approach to overcome these limitations.

Generation and epitope mapping of novel neutralizing monoclonal antibodies against glycoprotein E2 of CSFV.

Classical swine fever virus (CSFV) is a highly contagious and economically important pathogen threatening pig industry worldwide, the envelope glycoprotein E2 of CSFV is the dominant antigen inducing strong antiviral neutralizing immunity. In this study, 7 monoclonal antibodies (mAbs) with neutralizing potency were generated using E2 protein of CSFV Shimen strain (SM) expressed by eukaryotic cells. Their reactivity with 116 CSFV strains in cell cultures and E2 proteins of 10 subgenotypes in western blots showed different CSFV spectrums they recognized.

Pleasant Odor Decreases Mouse Anxiety-like Behaviors by Regulating Hippocampal Endocannabinoid Signaling.

Anxiety disorder is one of the most common neuropsychiatric disorders, and affects many people's daily activities. Although the pathogenesis and treatments of anxiety disorder have been studied for several decades, the underlying mechanisms remain elusive. Here, we provide evidence that olfactory stimuli with inhaled linalool or 2-phenylethanol decreased mouse anxiety-like behaviors and increased the activities of hippocampal dentate granule cells (DGCs).

Phototheranostic LPP-QDs-IR-820 Nanocomposites for Specific NIR-II Imaging of Lymphatic and Photothermal Therapy of Cervical Tumors.

Precise theranostics of tumors is intricately linked to the early detection and monitoring of lymph nodes (LN) and metastases, making the targeted localization of LNs essential for tumor identification. However, designing LN-targeting probes remains a significant challenge due to issues such as lymphatic uptake, biocompatibility, and fluorescence stability. To address these challenges, near-infrared II (NIR-II) fluorescence probes are developed through meticulous analysis of LN physiological structure and passive targeting strategy for LN detection and tumor therapy.

Targeted Delivery of AR-V7 siRNA with Bivalent PSMA Aptamers Effectively Suppresses the Growth of Enzalutamide-Resistant Prostate Cancer.

Androgen deprivation therapy has been the primary treatment strategy for advanced prostate cancer (PCa). But most patients develop castration resistance over time. For FDA-approved second-generation androgen receptor (AR) antagonists, including enzalutamide (ENZ) and abiraterone (AA), patients who initially respond to them eventually develop resistance.

Palmitic acid inhibits macrophage-mediated chemotherapy resistance in multiple myeloma via ALOX12 signaling.

We previously discovered that macrophages (MΦs), especially tumor-associated MΦs (tMΦs), contribute to chemotherapy resistance in multiple myeloma (MM). However, the mechanism underlying MΦ-mediated chemotherapy resistance in MM needs further elucidation, and the identification of factors that preferentially abrogate MΦ-induced inhibition of MM chemotherapy may have important clinical significance. In this study, we showed that the expression of FASN and SCD2, the enzymes that synthesize palmitic acid and convert it to palmitoleic acid, was decreased in tMΦs compared with MΦs.