Curvularin derivatives from hydrothermal vent sediment fungus Penicillium sp. HL-50 guided by molecular networking and their anti-inflammatory activity.

Guided by molecular networking, nine novel curvularin derivatives (1-9) and 16 known analogs (10-25) were isolated from the hydrothermal vent sediment fungus Penicillium sp. HL-50. Notably, compounds 5-7 represented a hybrid of curvularin and purine.

Amoenucles A-F, novel nucleoside derivatives with TNF-α inhibitory activities from Aspergillus amoenus TJ507.

Amoenucles A-F (1-6), six previously undescribed nucleoside derivatives, and two known analogs (7 and 8) were isolated from the culture of Aspergillus amoenus TJ507. Their structures were elucidated through spectroscopic analysis, single-crystal X-ray crystallography, and chemical reactions. Notably, 3 and 4 represent the first reported instances of nucleosides with an attached pyrrole moiety.

Se-methylselenocysteine Inhibits Migration and Glycolysis in Anaplastic Thyroid Carcinoma Cells via the ERK1/2 Signaling Pathway .

Objective: The aim of the present study was to determine the effects of selenium-methylselenocysteine (MSC) on the viability, migration, and glycolysis of human ATC cell lines 8305 and BHT101 .

Methods: Cells were treated with MSC and viability was determined using the Cell Counting Kit 8 assay. The migratory ability of cells was detected using a Transwell migration assay, and the expression levels of proteins involved in the ERK1/2, JNK, and p38 signaling pathways were measured by western blotting.

[Establishment and application of a genetic operating system in for the synthesis of tetraacetyl phytosphingosine].

(.), an unconventional heterothallic yeast species, is renowned for its high production of tetraacetyl phytosphingosine (TAPS). Due to its excellent performance in TAPS production, this study aimed to construct a genetic operating system of .

[Enzymatic MBH reaction catalyzed by an artificial enzyme designed with the introduction of an unnatural tertiary amine cofactor].

As the chip of synthetic biology, enzymes play a vital role in the bio-manufacturing industry. The development of diverse functional enzymes can provide a rich toolbox for the development of synthetic biology. This article reports the construction of an artificial enzyme with the introduction of a non-natural cofactor.

[Metabolic engineering of for the biosynthesis of O-acetyl-L-homoserine].

O-acetyl-L-homoserine (OAH) is a promising platform compound for the production of L-methionine and other valuable compounds, while its low yield and low conversion rate limit the industrial application. To solve these problems, we constructed a strain for high OAH production with the previously constructed L-homoserine producer HS33 as the chassis by systematic metabolic engineering. Firstly, PEP accumulation, pyruvate utilization, and OAH synthesis pathway (overexpressing , , and ) were enhanced to obtain an initial strain accumulating 13.

Novel anionic functionalized magnetic β-cyclodextrin composites with excellent adsorption capacity for moxifloxacin and wide pH adaptive adsorption capability for copper ion.

Antibiotics and heavy metals pose severe risks to human health and ecological environment. Therefore, developing a multifunctional adsorbent to remove these contaminants from wastewater is an urgent need. Herein, novel anionic sulfonic acid groups functionalized magnetic β-cyclodextrin (β-CD) composites (FCD@AA) were synthesized by coating poly(2-acrylamido-2-methyl-1-propane sulfonic acid (AMPS)) on the surface of magnetic β-CD particles (FCD).

Eleutherococcus senticosus Alleviates Aristolochic-Acid-Induced Acute Kidney Damage by Inhibiting the NLRP3/IL-1β Signaling Pathway in Mice.

(ES) exerts various pharmacological effects, including renoprotection in a rat model of renal ischemia-reperfusion injury. However, the mechanisms of these effects remain unclear. Therefore, we investigated the effects and mechanisms of ES on aristolochic acid (AA)-induced acute kidney injury in mice.

Selective gold extraction from e-waste leachate via sulfur-redox mechanisms using sulfhydryl-functionalized MOFs.

Urban mining of precious metals from electronic waste (e-waste) offers a dual advantage by addressing solid waste management challenges and supplying high-value metals for diverse applications. However, traditional extraction methods generally suffer from poor selectivity and limited capacity in complex acidic leachate. Herein, we present a sulfhydryl-functionalized zirconium-based metal-organic framework (Zr-MSA-AA) as a recyclable and highly selective adsorbent for efficient gold recovery.

Associations between 25-hydroxyvitamin D/calcium/alkaline phosphatase levels and the risk of developing kidney stones: Results from NHANES (2013-2018)-based and Mendelian randomization studies.

This study explores the relationship between 25-hydroxyvitamin D/calcium/alkaline phosphatase (ALP) levels and kidney stone development via cross-sectional and Mendelian randomization (MR) analyses. We used data from the National Health and Nutrition Examination Survey (NHANES) 2013 to 2018 to explore the associations of 25(OH)D metabolite, calcium, and ALP levels with kidney stone development, LDSC analysis to determine the associations between their genetically predicted levels and kidney stone development, and MR analysis to determine the causality of those relationship via genome-wide association studies (GWASs). The cross-sectional study revealed a relationship between ALP levels and kidney stone development (Model 1: OR = 1.

BMI-mediated association between glyphosate exposure and increased risk of atherosclerotic heart disease: A large-scale cross-sectional study.

Background: Glyphosate, as the main component of glyphosate pesticides, has been shown to have toxic effects on multiple human systems. However, the association between glyphosate and atherosclerotic cardiovascular disease (ASCVD) remains unclear. This study aims to explore the effect of glyphosate exposure on ASCVD.

α-Glucosidase inhibitory activities of polyphenols from Mesua ferrea L. leaves.

Mesua ferrea L. is used in Ayurvedic and Thai medicine for treating various diseases, including diabetes. This study aimed to isolate and identify the bioactive constituents from M.

Research progress on N6-methyladenosine RNA modification in osteosarcoma: functions, mechanisms, and potential clinical applications.

Osteosarcoma (OS) is the most commonly diagnosed primary malignant bone tumor in children and adolescents. Despite significant advancements in therapeutic strategies against OS over the past few decades, the prognosis for this disease remains poor, largely due to its high invasiveness and challenges associated with its treatment. N6-methyladenosine (m6A) modification is one of the most abundant epigenetic modifications of RNAs, and many studies have highlighted its crucial role in OS.

Aloin remodels the cell wall of Candida albicans to reduce its hyphal virulence against oral candidiasis.

Aloe vera (L.) Burm.f.

Dynamics and regulatory roles of RNA mA methylation in unbalanced genomes.

-methyladenosine (mA) in eukaryotic RNA is an epigenetic modification that is critical for RNA metabolism, gene expression regulation, and the development of organisms. Aberrant expression of mA components appears in a variety of human diseases. RNA mA modification in has proven to be involved in sex determination regulated by and may affect X chromosome expression through the MSL complex.

Association Between Urinary Metal Levels and Chronic Kidney Dysfunction in Rural China: A Study on Sex-Specific Differences.

Background: While current epidemiological studies have documented associations between environmental metals and renal dysfunction, the majority have concentrated on plasma metal levels. The relationship between urinary metal exposure and chronic kidney disease (CKD) remains contentious, particularly within specific demographic groups.

Methods: This cross-sectional study included 2919 rural Chinese adults recruited between 2018 and 2019.

Biological Characteristics and Whole-Genome Analysis of a Porcine Phage.

(1) Background: In recent years, the increasing emergence of multidrug-resistant pathogens in pig farms has begun to pose a severe threat to animal welfare and, by extension, public health. In this study, we aimed to explore the biological characteristics and genomic features of bacteriophages that are capable of lysing porcine multidrug-resistant , which was isolated from sewage. In doing so, we provided a reference for phage therapies that can be used to treat multidrug-resistant strains.

In Vitro Evaluation of Ruminal Digestibility, Fermentation Characteristics, and Bacterial Diversity of Kenaf Crop at Various Cutting Heights.

The current study investigated the in vitro degradability, in vitro gas production, methane (CH) production, and ruminal bacterial community of kenaf plants cut at different heights (130, 160, 190, 220, and 250 cm). These samples were subjected to an in vitro batch culture system using buffalo rumen fluid to measure gas and CH production at 3, 6, 9, 12, 24, 36, 48, and 72 h of incubation. Results reveal that crude protein (CP) concentration was the highest at the 220 cm height compared with the other heights.

A Study on the Induction of Multi-Type Immune Responses in Mice via an mRNA Vaccine Based on Hemagglutinin and Neuraminidase Antigen.

Background: The Influenza A virus (IAV), a pathogen affecting the respiratory system, represents a major risk to public health worldwide. Immunization remains the foremost strategy to control the transmission of IAV. The virus has two primary antigens: hemagglutinin (HA) and neuraminidase (NA).

The Comorbidity of Depression and Diabetes Is Involved in the Decidual Protein Induced by Progesterone 1 (Depp1) Dysfunction in the Medial Prefrontal Cortex.

Background: There is a high rate of depressive symptoms such as irritability, anhedonia, fatigue, and hypersomnia in patients with type 2 diabetes mellitus (T2DM). However, the causes and underlying mechanisms of the comorbidity of depression and diabetes remain unknown.

Methods: For the first time, we identified Decidual protein induced by progesterone 1 (Depp1), also known as DEPP autophagy regulator 1, as a hub gene in both depression and T2DM models.

CRISPR/cas9 Allows for the Quick Improvement of Tomato Firmness Breeding.

Fruit firmness is crucial for storability, making cultivating varieties with higher firmness a key target in tomato breeding. In recent years, tomato varieties primarily rely on hybridizing ripening mutants to produce F hybrids to enhance firmness. However, the undesirable traits introduced by these mutants often lead to a decline in the quality of the varieties.

BSA-Assisted Synthesis of Au Nanoclusters/MnO Nanosheets for Fluorescence "Switch-On" Detection of Alkaline Phosphatase.

A fluorescence probe for "switch-on" detection of alkaline phosphatase (ALP) was developed based on Au nanoclusters anchored MnO nanosheets (Au NCs-MnO NSs), which were synthesized using bovine serum albumin (BSA) as template through a simple one-pot approach. In the sensing system, MnO NSs function as both energy acceptors and target identifiers, effectively quenches the fluorescence of Au NCs via fluorescence resonance energy transfer (FRET). The presence of ALP catalyzes the hydrolysis of L-ascorbic acid-2-phosphate (AAP) to ascorbic acid (AA), reducing MnO NSs to Mn and facilitate the fluorescence recovery of Au NCs.

Multi-Strategy Improved Whale Optimization Algorithm and Its Engineering Applications.

The Whale Optimization Algorithm (WOA) is recognized for its simplicity, few control parameters, and effective local optima avoidance. However, it struggles with global search efficiency and slow convergence. This paper introduces the Improved WOA (ImWOA) to overcome these challenges.

A chromosome-scale genome assembly of the pioneer plant Stylosanthes angustifolia: insights into genome evolution and drought adaptation.

Background: Drought is a major limiting factor for plant survival and crop productivity. Stylosanthes angustifolia, a pioneer plant, exhibits remarkable drought tolerance, yet the molecular mechanisms driving its drought resistance remain largely unexplored.

Results: We present a chromosome-scale reference genome of S.

Ultralow Power Cold-Fuse Memory Based on Metal-Oxide-CNT Structure.

One-time programmable (OTP) memory is an essential component in chips, which has extremely high security to protect the stored critical information from being altered. However, traditional OTP memory based on the thermal breakdown of the dielectric has a large programming current, which leads to high power consumption. Here, we report a gate tunneling-induced "cold" breakdown phenomenon in carbon nanotube (CNT) field-effect transistors, and based on this we construct a "cold" fuse (C-fuse) memory where applying a mild gate voltage can break down the CNT channel without damaging the gate dielectric.