Substrate Specificity of Adenine-Cu-PO Nanozyme: Ascorbic Acid Oxidation and Selective Cytotoxicity.

Though nanozymes are becoming promising alternatives to natural enzymes due to their superior properties, constructing nanozyme with high specificity is still a great challenge. Herein, with Cu as an active site and adenine as a ligand, Adenine-Cu-PO is synthesized in phosphate-buffered saline. As an oxidase mimic, Adenine-Cu-PO could selectively catalyze oxidation of ascorbic acid (AA) to dehydroascorbic acid, but not universal substrates (3,3',5,5'-tetramethylbenzidine (TMB), 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) and 2,4-dichlorophenol (2,4-DP)), small biomolecules (dopamine, glutathione, glucose, galactose), other vitamins (vitamin A acid, vitamin B, vitamin K) and even dithiothreitol (a common interference of AA).

Identification of novel CDH23 heterozygous variants causing autosomal recessive nonsyndromic hearing loss.

Background: Hearing loss adversely impacts language development, acquisition, and the social and cognitive maturation of affected children. The hearing loss etiology mainly includes genetic factors and environmental factors, of which the former account for about 50-60%.

Objective: This study aimed to investigate the genetic basis of autosomal recessive non-syndromic hearing loss (NSHL) by identifying and characterizing novel variants in the CDH23 gene.

Sphingosine-1-phosphate in the regulation of diabetes mellitus: a scientometric study to an in-depth review.

Diabetes is a significant global health issue, causing extensive morbidity and mortality, and represents a serious threat to human health. Recently, the bioactive lipid molecule Sphingosine-1-Phosphate has garnered considerable attention in the field of diabetes research. The aim of this study is to comprehensively understand the mechanisms by which Sphingosine-1-Phosphate regulates diabetes.

Diagnostic and Predictive Recurrence Value of Plasma Fibrinogen in Patients With Adrenocortical Carcinoma.

Background: The correlation between fibrinogen levels and adrenocortical carcinoma (ACC) remains unclear. This study aimed to explore the value of preoperative plasma fibrinogen as a biomarker for ACC.

Methods: We identified 40 patients with ACC and 170 patients with adrenal adenoma (AA) who underwent surgery at our institution between 2015 and 2022.

L. protects cerebral ischemia/reperfusion injury via arachidonic acid/p53-mediated apoptosis axis.

Introduction: Stroke is a debilitating disease and the second leading cause of death worldwide, of which ischemic stroke is the dominant type. L., also known as safflower, has been used to treat cerebrovascular diseases, especially ischemic stroke in many Asian countries.

Plasma p-tau and neurofilament/p-tau ratio in differentiating Alzheimer's disease from syndromes associated with frontotemporal lobar degeneration.

Introduction: Plasma-based biomarkers have shown promise for clinical implementation, but their accuracy in differentiating Alzheimer's disease (AD) from syndromes associated with frontotemporal lobar degeneration (FTLD) has yet to be fully investigated. This study assessed the potential of plasma biomarkers for differential diagnosis.

Methods: This cohort study included 374 participants (96 AD, 278 FTLD).

A Real-World Pharmacovigilance Study of FDA Adverse Event Reporting System Events for Obeticholic Acid.

Background And Objectives: Based on the Adverse Event Reporting System (FAERS) data from the US FDA, this study mined the adverse drug reactions of obeticholic acid (OCA) in the real world and provided reference for clinical safe drug use.

Methods: Adverse event reports for OCA from the second quarter of 2016 to the third quarter of 2023 were extracted. The analysis for adverse reaction signal detection was conducted using reporting odds ratio, proportional reporting ratio, Bayesian confidence propagation neural network, and multi-item gamma Poisson shrinker methods.

Comparative analysis of improved m6A sequencing based on antibody optimization for low-input samples.

The most effective method for mapping N6-methyladenosine (mA) is mA RNA immunoprecipitation sequencing (MeRIP-seq). The quality of MeRIP-seq relies on various factors, with the anti-mA antibody being a crucial determinant. However, comprehensive research on anti-mA antibody selection and optimal concentrations for different tissues has been limited.

The association between NADPH oxidase (NOX) polymorphisms with immunohistochemistry and survival in diffuse large B cell lymphoma patients.

The purpose of this study was to comprehensively analyze the prediction role of NADPH oxidase (NOX)-related polymorphisms (NCF4: rs1883112, CYBA: rs4673, RAC2: rs13058338) and immunohistochemical indices on survival in diffuse large B-cell lymphoma (DLBCL).The impact of NOX polymorphisms were evaluated in 335 DLBCL patients treated with R (rituximab)-CHOP (cyclophosphamide, doxorubicin, vincristine, and prednisone) from Harbin Medical University Cancer Hospital. We also collected information on their immunohistochemical expression and clinical outcomes.

Genomic prediction accounting for dominance and epistatic genetic effects on litter size traits in Large White pigs.

Litter size traits of sows are crucial for the economic benefits of the pig industry. Three phenotypic traits of 1,206 Large White (LW) pigs, that is, the total number born (TNB), number born alive (NBA), and number of healthy piglets (NHP), were recorded. We evaluated a series of genomic best linear unbiased prediction (GBLUP) models that sequentially added additive effects (model A), dominance effects (model A+D), and epistatic effects (model A+D+AA, model A+D+AA+AD, and model A+D+AA+AD+DD) using chip data and imputed whole-genome sequencing (WGS) data to estimate genetic parameters and predictive accuracy.

The Correlation Between Fecal Amino Acids, Colonic Mucosal Taste Receptors, and Clinical Features and Indicators of Ulcerative Colitis: A Multicenter Exploratory Study.

Background: Patients with ulcerative colitis (UC) exhibit abnormal amino acid (AA) metabolism. Taste receptors play a crucial role in the detection of intestinal AAs. Nevertheless, it remains unclear whether UC patients exhibit abnormal expression of these receptors in the colon.

Idebenone Protects Photoreceptors Impaired by Oxidative Phosphorylation Disorder in Retinal Detachment.

Purpose: Oxidative phosphorylation (OXPHOS) is an aerobic metabolic mechanism, and its dysfunction plays an important role in the pathological changes of ischemic diseases. However, systematic studies on the occurrence of retinal detachment (RD) are lacking.

Methods: Single-cell RNA sequencing (scRNA-seq) of the human retina was performed to detect the metabolic changes of various retinal cells after RD.

The associations between dietary advanced glycation-end products intake and self-reported infertility in U.S. women: data from the NHANES 2013-2018.

Evidence suggest that dietary advanced glycation end products (AGEs) might exert harmful effects on female reproductive function. However, population-based studies exploring the associations between dietary AGEs intake and female infertility remain lacking. This studyaimed to determine the relationship between dietary AGEs intake and female infertility based on National Health and Nutrition Examination Survey (NHANES).

Xalnesiran with or without an Immunomodulator in Chronic Hepatitis B.

Background: Xalnesiran, a small interfering RNA molecule that targets a conserved region of the hepatitis B virus (HBV) genome and silences multiple HBV transcripts, may have efficacy, with or without an immunomodulator, in patients with chronic HBV infection.

Methods: We conducted a phase 2, multicenter, randomized, controlled, adaptive, open-label platform trial that included the evaluation of 48 weeks of treatment with xalnesiran at a dose of 100 mg (group 1), xalnesiran at a dose of 200 mg (group 2), xalnesiran at a dose of 200 mg plus 150 mg of ruzotolimod (group 3), xalnesiran at a dose of 200 mg plus 180 μg of pegylated interferon alfa-2a (group 4), or a nucleoside or nucleotide analogue (NA) alone (group 5) in participants with chronic HBV infection who had virologic suppression with NA therapy. The primary efficacy end point was hepatitis B surface antigen (HBsAg) loss (HBsAg level, <0.

Evaluation of Auricular Acupressure on Myopia Prevention among Children Aged 6-12 Years with Pre-Myopia in China: Study Protocol of a Prospective Multi-Center Randomized Controlled Trial.

Background: The increasing prevalence of myopia among Chinese children and adolescents, especially at younger ages, has emerged as a significant concern in recent years. Pre-myopia is a key period for myopia prevention and control in children and adolescents. Previous studies suggested auricular acupressure (AA) therapy might offer a viable approach to prevent and slow down myopia progression.

Tuning into urban birdsong: enhancing nature connectedness with an AI-powered wearable.

The rapid urbanization has led to the loss of natural spaces and a subsequent disconnection between humans and nature, negatively affecting residents' well-being and environmental awareness. There is a a growing interest in leveraging technology to address this gap in Human-Computer Interaction. This article introduces GoChirp, an AI-powered wearable device for enhancing nature relatedness within urban landscapes.

Pelvic Desmoplastic Small Round Cell Tumor on 18F-PSMA PET/CT.

Desmoplastic small round cell tumor (DSRCT) as a subtype of sarcoma is rare and aggressive. We present the case of a 23-year-old man with a histologically confirmed DSRCT who underwent 18F-PSMA PET/CT before prostate biopsy and surgical resection of the tumor. It is well known that PSMA PET has a high accuracy for detecting clinically significant prostate adenocarcinoma and is useful in guiding prostate biopsy.

Sphingosine kinase 2 (SphK2) depletion alters redox metabolism and enhances inflammation in a diet-induced MASH mouse model.

Background: Sphingosine-1 phosphate (S1P) is a bioactive lipid molecule that modulates inflammation and hepatic lipid metabolism in MASLD, which affects 1 in 3 people and increases the risk of liver fibrosis and hepatic cancer. S1P can be generated by 2 isoforms of sphingosine kinase (SphK). SphK1 is well-studied in metabolic diseases.

Safety and efficacy of lamivudine/dolutegravir vs. bictegravir/emtricitabine/tenofovir alafenamide in antiretroviral-naive adults with HIV-1 infection in Shanghai, China: a single-centre retrospective study.

Lamivudine plus dolutegravir (3TC/DTG) and bictegravir/emtricitabine/tenofovir alafenamide (B/F/TAF) regimens are commonly used as first-line treatments for people living with human immunodeficiency virus (HIV) (PLWH) worldwide. There are limited comparative data on the antiviral activity and safety between these regimens in ART-naive PLWH, particularly in China, where the 3TC/DTG regimen was integrated into first-line therapy in 2021 and gained broader adoption after its inclusion in the National Health Insurance in 2022. This study aims to provide real-world evidence comparing the 3TC/DTG regimen to the B/F/TAF regimen in ART-naive PLWH in China.

Remimazolam for the prevention of emergence agitation in adults following nasal surgery under general anesthesia: a prospective randomized clinical controlled trial.

Background: Remimazolam is a novel intravenous sedative/anesthetic drug that belongs to the ultra-short-acting class of benzodiazepines. The purpose of this study was to evaluate the effectiveness of postoperative use of remimazolam in preventing emergence agitation (EA) in adults following nasal surgery.

Methods: Patients who underwent nasal surgery were randomly divided into Group R and Group C.

Examining the progress and implementation of neonatal peritoneal dialysis in the Ningxia autonomous region.

Background: Neonates are prone to experiencing acute kidney injury (AKI) and metabolic irregularities. Although hemodialysis is a primary treatment for these conditions, its utilization is not prevalent in the Ningxia Autonomous Region in China. Peritoneal dialysis (PD) presents itself as an alternative with benefits such as simplicity, cost-effectiveness, and minimal technical complexity compared to hemodialysis.

Mechanical Twisting-Induced Enhancement of Second-Order Optical Nonlinearity in a Flexible Molecular Crystal.

Flexible molecular crystals are essential for advancing smart materials, providing unique functionality and adaptability for applications in next-generation electronics, pharmaceuticals, and energy storage. However, the optical applications of flexible molecular crystals have been largely restricted to linear optics, with nonlinear optical (NLO) properties rarely explored. Herein, we report on the application of mechanical twisting of flexible molecular crystals for second-order nonlinear optics.

Avermectins and Their Derivatives: Recent Advances in Biosynthesis and Application.

Avermectins (AVMs) and their derivatives are the most effective and widely used nematicides, insecticides, and acaricides against endo- and ectoparasites of plants, animals, and humans. Demand for avermectins and their highly effective derivatives has increased due to their high cost-effectiveness and wide range of applications as medicines and crop protection products. Due to the unique structures of these compounds and for industrial production purposes, numerous efforts and strategies have been dedicated to enhancing the production of avermectins and creating new analogues in recent years.

Pharmacokinetics and Tissue Distribution of Albendazole and Its Three Metabolites in Yellow River Carp () after Single Oral Administration.

This study aimed to evaluate the pharmacokinetics and tissue distribution of albendazole (ABZ) and its three metabolites─albendazole sulfoxide (ABZSO), albendazole sulfone (ABZSO), and albendazole-2-aminosulfone (ABZ-2-NH-SO)─in Yellow River carp () reared at 17.2 ± 1.1 °C after single oral administration of 12 mg/kg body weight (BW) ABZ.

Construction of an Efficient -Succinyl--homoserine Producing Cell Factory and Its Application for Coupling Production of -Methionine and Succinic Acid.

-Succinyl--homoserine (OSH) is an important C4 platform compound with broad applications. Its green and efficient production is receiving increasing attention. Herein, the OSH producing chassic cell was constructed by deleting the transcriptional negative regulation factor, blocking the OSH consumption pathway, and inhibiting the competitive bypass pathways.