Enhancing soybean germination and vigor under water stress: the efficacy of bio-priming with sodium carboxymethyl cellulose and gum arabic.

Seed priming can significantly enhance the tolerance of soybean against different environmental stresses by improving seed water uptake and modulating stress-response mechanisms. In particular, seed priming with sodium carboxymethylcellulose (SCMC) and gum Arabic (GA) can support seeds to withstand extreme conditions better, promoting more consistent germination and robust seedling establishment, which is crucial for achieving stable agricultural yields. The present study investigated the effects of seed priming using a combination of SCMC and GA (10% CG) on the germination, growth, and biochemical responses of six soybean varieties under drought and flooding stress conditions.

Do delayed responses introduce bias in ecological momentary assessment? Evidence from comparisons between self-reported and objective physical activity.

Introduction: Delayed responses are a common yet often overlooked aspect of participant compliance in ecological momentary assessment (EMA) research. This study investigated whether response delays introduce selection bias in the moments captured by EMA.

Methods: Participants ( = 339) self-reported their physical activity behaviors using EMA five times a day over 7 days while wearing a continuous physical activity monitor.

Associations Among Emotion Regulation, COVID Stress, Alcohol Use, and Intimate Partner Aggression Among College Students During the COVID-19 Pandemic.

Purpose: Intimate partner aggression (IPA) increased after the onset of the COVID-19 pandemic. Following the Vulnerability-Stress-Adaptation (VSA) model, the current study examined associations among emotion regulation (ER) difficulties (an enduring vulnerability), COVID stress (a current stressor), alcohol use (a maladaptive coping strategy), and physical, psychological, and cyber IPA perpetration during the first eight months of the pandemic.

Method: Participants were 215 college students in current relationships from three universities across the US.

A tactfully designed photothermal agent collaborating with ascorbic acid for boosting maxillofacial wound healing.

Maxillofacial injuries that may cause severe functional and aesthetic damage require effective and immediate management due to continuous exposure to diverse microbial populations. Moreover, drug resistance, biofilm formation, and oxidative stress significantly impede timely bacterial removal and immune function, making the exploration of advanced materials for maxillofacial wound healing an appealing yet highly challenging task. Herein, a near-infrared photothermal sterilization agent was designed, encapsulated with liposomes and coated with ascorbic acid known for its antioxidant and immune-regulatory functions.

Manipulated Slow Release of Florfenicol Hydrogels for Effective Treatment of Anti-Intestinal Bacterial Infections.

Objective: The difficulty of establishing slow release at intestinal infection sites, weak antibacterial effects, as well as the limited broad use of florfenicol oral formulations are the main targets of the current study. Novel hydrogels derived from sodium alginate were developed using a complexation form for florfenicol delivery to achieve slow release at the site of intestinal infection and enhance its antibacterial activity against .

Methods: The optimal formulation, physicochemical properties, stability, pH-responsive performance, antibacterial activity, and in vitro biosafety of the florfenicol hydrogels have been studied systematically.

Characterizing the extractable proteins from tomato leaves - A proteomics study.

The ambition to utilize agricultural by-products has spotlighted tomato leaves as a promising source for plant-based proteins. High-yielding protein extractability is key for its industrial use, but previous studies reported decreased protein extractability at later stages of plant development. This study investigated the underlying factors in protein extractability through a comprehensive proteomics analysis across four plant developmental stages (vegetative, flowering, fruit-forming, mature-fruit).

Maturation of striatal dopamine supports the development of habitual behavior through adolescence.

Developmental trajectories during the transition from adolescence to adulthood contribute to the establishment of stable, adult forms of operation. Understanding the neural mechanisms underlying this transition is crucial for identifying variability in normal development and the onset of psychiatric disorders, which typically emerge during this time. Habitual behaviors can serve as a model for understanding brain mechanisms underlying the stabilization of adult behavior, while also conferring risk for psychopathologies.

Outcomes of a non-diffractive extended depth of focus intraocular lens in patients with well-controlled glaucoma and ocular hypertension.

Aim: To assess visual outcomes and satisfaction of a non-diffractive extended depth of focus (EDOF) intraocular lens (IOL) in individuals with ocular hypertension (OHT) and well-controlled mild glaucoma undergoing cataract surgery.

Methods: An investigator-initiated, single-center, prospective, interventional, noncomparative study conducted in Montreal, Canada. The study enrolled 31 patients (55 eyes) with OHT or mild glaucoma who received a non-diffractive EDOF IOL (Acrysof IQ Vivity).

Hypoxia-Inducible Factor-1α Regulates BNIP3-Dependent Mitophagy and Mediates Metabolic Reprogramming Through Histone Lysine Lactylation Modification to Affect Glioma Proliferation and Invasion.

Objective: Gliomas are the predominant form of malignant brain tumors. We investigated the mechanism of hypoxia-inducible factor-1α (HIF-1α) affecting glioma metabolic reprogramming, proliferation and invasion.

Methods: Human glioma cell U87 was cultured under hypoxia and treated with small interfering (si)HIF-1α, si-B cell lymphoma-2/adenovirus E1B 19-kDa interacting protein 3 (siBNIP3), si-YT521-B homology domain 2 (siYTHDF2), 3-methyladenine and 2-deoxyglucose, with exogenous sodium lactate-treated normally-cultured cells as a lactate-positive control.

Robust-adhesion and high-mechanical strength hydrogel for efficient wet tissue adhesion.

Bioadhesive hydrogels show great promise in wound closure due to their minimally invasive nature and ease of use. However, they typically exhibit poor wet adhesion and mechanical properties on wet tissues. Herein, a ready-to-use bioadhesive hydrogel (denoted as PAA-NHS/C-CS) with rapidly robust adhesion and high mechanical strength is developed a simple one-pot UV crosslinking polymerization of acrylic acid (AA), catechol-functionalized chitosan (C-CS), and acrylic acid -hydroxysuccinimide ester (AA-NHS ester).

Preliminary effects of oral ANS-6637, an ALDH2 inhibitor, on cue-induced craving, safety and alcohol consumption among adults with alcohol use disorder: a proof-of-concept, randomized, human laboratory trial.

Aims: We evaluated the safety, efficacy, and patient adherence to oral ANS-6637, a selective, reversible inhibitor of aldehyde dehydrogenase 2 (ALDH2), for treating alcohol use disorder (AUD).

Methods: A 3-arm, double-blind, randomized, proof-of-concept human laboratory study embedded in a 5-week multisite clinical trial tested 200 mg and 600 mg daily doses of ANS-6637 compared to placebo in treatment-seeking adults with AUD. After 1 week of medication, participants completed an alcohol cue reactivity session.

Carbon-doped bimetallic oxide nanoflakes for simultaneous electrochemical analysis of ascorbic acid, uric acid, and acetaminophen in sweat.

Non-invasive continuous detection using tears or sweat as substitutes for blood samples has become an emerging method for real-time monitoring of human health. However, its development is limited by the low sample volume and low level of analytes. The simultaneous determination of multi-analytes with highly sensitive electrochemical sensing platforms has undoubtedly resulted in breakthrough innovations.

Low incidence of primary graft failure with bendamustine, fludarabine, and busulfan conditioning prior to haploidentical allogeneic hematopoietic cell transplantation.

The outcomes of haploidentical hematopoietic cell transplantation (haplo-HCT) have improved with the implication of new in vivo and ex vivo graft-versus-host disease (GVHD) prophylaxis regimens. However, primary graft failure is still reported more frequently in haplo-HCT compared to a matched donor HCT. We conducted a pilot study (NCT04942730) to evaluate the impact of adding bendamustine to fludarabine and busulfan conditioning on engraftment after haplo-HCT.

Comprehensive Analysis of m6A-Related Programmed Cell Death Genes Unveils a Novel Prognostic Model for Lung Adenocarcinoma.

Lung adenocarcinoma (LUAD) involves complex dysregulated cellular processes, including programmed cell death (PCD), influenced by N6-methyladenosine (m6A) RNA modification. This study integrates bulk RNA and single-cell sequencing data to identify 43 prognostically valuable m6A-related PCD genes, forming the basis of a 13-gene risk model (m6A-related PCD signature [mPCDS]) developed using machine-learning algorithms, including CoxBoost and SuperPC. The mPCDS demonstrated significant predictive performance across multiple validation datasets.

Tolterodine is a novel candidate for assessing CYP3A4 activity through metabolic volatiles to predict drug responses.

Cytochrome P450 (CYP) 3A4 plays a major role in drug metabolism. Its activity could be determined by non-invasive and cost-effective assays, such as breath analysis, for the personalised monitoring of drug response. For the first time, we identify an isotopically unlabelled CYP3A4 substrate, tolterodine that leads to the formation of a non-toxic volatile metabolite, acetone, which could potentially be applied to monitor CYP3A4 activity in humans.

Proteomic analysis reveals the roles of silicon in mitigating glyphosate-induced toxicity in Brassica napus L.

Glyphosate (Gly) is a widely used herbicide for weed control in agriculture, but it can also adversely affect crops by impairing growth, reducing yield, and disrupting nutrient uptake, while inducing toxicity. Therefore, adopting integrated eco-friendly approaches and understanding the mechanisms of glyphosate tolerance in plants is crucial, as these areas remain underexplored. This study provides proteome insights into Si-mediated improvement of Gly-toxicity tolerance in Brassica napus.

Investigation of dielectric and spectroscopic properties of pure, commercial, and sugar solution added honey with microwave X band and spectroscopy techniques.

The adulteration of honey is a globally growing issue due to its medicinal benefits and health-promoting properties. This problem primarily involves the addition of sugars and other substances. To address these concerns, a comparative study was conducted to investigate the dielectric and spectroscopic properties of pure, sugar solution added honey, and commercially available honey.

[Development and Validation of an Analytical Method for 2-Thiouracil, 4-Thiouracil, and 6-Methyl-2-thiouracil in Bovine Urine: A Method for Monitoring Inspections in Beef Exports to the European Union].

Thiouracil (2-thiouracil) is a thyrostat used to promote weight gain in cattle. However, its use is prohibited within the European Union (EU), necessitating the monitoring of its presence in bovine urine for beef exports to the EU. In this study, we present the development and validation of a quantitative method for the determination of 2-thiouracil, 4-thiouracil, and 6-methyl-2-thiouracil in bovine urine using liquid chromatography-tandem mass spectrometry (LC-MS/MS).

Insights into nitrogen metabolism and humification process in aerobic composting facilitated by microbial inoculation.

To enhance the retention of compost nutrients, specifically in nitrogen metabolism and humification, compound microbial agents were added during the aerobic composting of bagasse pith and buffalo manure. The introduction of the microbial agent successfully colonized the mixture, boosted the degradation capacity of organic matter, and facilitated the formation of nitrogenous substances and humic substances (HSs). The incorporation of a composite microbial inoculum led to a substantial rise in total Kjeldahl nitrogen (TKN) by 62.

Conformationally adaptive therapeutic peptides for diseases caused by intrinsically disordered proteins (IDPs). New paradigm for drug discovery: Target the target, not the arrow.

The traditional model of protein structure determined by the amino acid sequence is today seriously challenged by the fact that approximately half of the human proteome is made up of proteins that do not have a stable 3D structure, either partially or in totality. These proteins, called intrinsically disordered proteins (IDPs), are involved in numerous physiological functions and are associated with severe pathologies, e.g.

Semicircular-aperture illumination scanning transmission electron microscopy.

Scanning transmission electron microscopy (STEM) provides high-resolution visualization of atomic structures as well as various functional imaging modes utilizing phase contrasts. In this study we introduce a semicircular aperture in STEM bright field imaging, which gives a phase contrast transfer function that becomes complex and includes both lower and higher spatial frequency contrast transfer. This approach offers significant advantages over conventional phase plate methods, having no charge accumulation, degradation, or unwanted background noise, which are all problematic in the phase plate material.

New series of fluoroquinolone derivatives as potential anticancer Agents: Design, Synthesis, in vitro biological Evaluation, and Topoisomerase II Inhibition.

A series of fluoroquinolone analogs (II, III) derived from Ciprofloxacin hydrazide were designed, and synthesized. The NCI-60 Human Tumor Cell Line Screening assay indicated that compounds II, III, and III are the most potent among the series and were further selected for five-dose evaluation, where they exhibited potent cytotoxicity with mean GI values of 3.30, 2.

Unraveling Various Stacking Modes and Local Structures in Poly(triazine imide) Materials via Low-Dose Electron Microscopy.

Poly(triazine imide) (PTI) materials, a class of layered graphitic carbon nitrides, have garnered significant attention for their unique electronic, thermal, and catalytic properties. These properties can be adjusted through postsynthesis treatments. However, the influence of these treatments on the layer stacking modes and local structures within PTI remains largely unexplored.

Inhibition of diacylglycerol lipase α induced blood-brain barrier breach in female Sprague-Dawley rats.

The endocannabinoid system's significance in maintaining blood-brain barrier (BBB) integrity under physiological and pathological conditions is suggested by several reports, but the underlying molecular mechanisms are not well understood. In this paper, we investigated the effects of depletion of 2-arachidonoylglycerol (2-AG), one of the main endocannabinoids in the central nervous system, on BBB integrity using pharmacological tools. Female Sprague-Dawley rats were injected with the diacylglycerol lipase α (DAGLα) inhibitor LEI-106 (40 mg/kg, i.

Role of Prothrombin and Methylenetetrahydrofolate Reductase Gene Polymorphisms as well as Thrombophilia Markers, as Risk Factors for Unexplained Recurrent Miscarriage: A Case-Control Study.

Background: Unexplained recurrent miscarriage (RM) is still an unsolved reproductive health problem. Inherited thrombophilias have been one of the causes. Mutation in genes encoding coagulation proteins, including prothrombin (PT G20210A) and methylenetetrahydrofolate reductase (MTHFR) genes, increase tendency for venous thromboembolism.