NLRP3 promoter methylation as a predictive biomarker for glucocorticoid response in patients with inflammatory bowel disease.

Glucocorticoids are used for inflammatory bowel disease (IBD) therapy; however nearly 50 % of IBD patients exhibit resistance or dependence. This study evaluates the relationship between methylation level at two CpG sites (cg21991396 and cg00448525) within NLRP3 promoter and glucocorticoid response of 94 IBD pediatrics (39 with Crohn's disease (40.4 %)) and 47 IBD adults (26 with Crohn's disease (55.

Poultry litter extract as solid waste supplement for enhanced microalgal biomass production and wastewater treatment.

Wastewater and livestock waste can be used as a cheap source of nutrients for microalgae growth. In this work, a cocktail waste medium (CWM) was developed using 75% Chhalera municipal wastewater (C-MWW), 25% Parag dairy wastewater (P-DWW), and 15 g L of poultry litter extract (PLE-15) for low-cost cultivation of Chlorella sp. BRE4.

Lignin-enabled ultra-stretchable eutectic gels for multifunctional sensors.

Eutectic gels as important conductive polymers have promising practical applications in wearable electronic devices. However, the development of the ultra-stretchable and self-adhesive eutectic gel for multifunctional flexible sensors remains a challenge. Here, a lignin-enabled ultra-stretchable eutectic gel (LEG) integrating with excellent self-adhesion and high conductivity is prepared through polymerizable deep eutectic solvents (PDES) treated lignin followed by in-situ polymerization.

Cross-species comparison reveals that Hmga1 reduces H3K27me3 levels to promote cardiomyocyte proliferation and cardiac regeneration.

In contrast to adult mammalian hearts, the adult zebrafish heart efficiently replaces cardiomyocytes lost after injury. Here we reveal shared and species-specific injury response pathways and a correlation between Hmga1, an architectural non-histone protein, and regenerative capacity, as Hmga1 is required and sufficient to induce cardiomyocyte proliferation and required for heart regeneration. In addition, Hmga1 was shown to reactivate developmentally silenced genes, likely through modulation of H3K27me3 levels, poising them for a pro-regenerative gene program.

Alkhumra Hemorrhagic Fever Virus (AHFV): A Concise Overview.

Alkhumra fever is a viral disease caused by the Alkhumra hemorrhagic fever virus (AHFV). It belongs to family , genus . AHFV is primarily transmitted to humans through the bite of infected ticks, for example, .

An perspective on the role of methylation-related genes in wheat - interaction.

Unlabelled: Wheat ( L.), a vital cereal crop, provides over 20% of the total calories and protein in the human diet. However, , the pathogen responsible for Fusarium head blight (FHB), poses a significant threat to wheat production by contaminating grains with harmful mycotoxins.

Dengue virus transmission in Italy: historical trends up to 2023 and a data repository into the future.

Dengue virus circulation is on the rise globally, with increased epidemic activity in previously unaffected countries, including within Europe. In 2023, global dengue activity peaked, and Italy reported the highest number of dengue cases and local chains of transmission to date. By curating several sources of information, we introduce a novel data repository focused on dengue reporting in Italy.

Revolutionizing cotton cultivation: A comprehensive review of genome editing technologies and their impact on breeding and production.

Cotton (Gossypium hirsutum L.), a vital global cash crop, significantly impacts both the agricultural and industrial sectors, providing essential fiber for textiles and valuable byproducts such as cottonseed oil and animal feed. The cultivation of cotton supports millions of livelihoods worldwide, particularly in developing regions, making it a cornerstone of rural economies.

Repeated dosing of AAV-mediated liver gene therapy in juvenile rat and mouse models of Crigler-Najjar syndrome type I.

Crigler-Najjar syndrome is an ultra-rare monogenic recessive liver disease caused by gene mutations. Complete UGT1A1 deficiency results in severe unconjugated hyperbilirubinemia in newborns that, if not treated, may lead to brain damage and death. Treatment is based on intensive phototherapy, but its efficacy decreases with age, rendering liver transplantation the only curative option.

Optimization of tissue culture and Cas9 transgene expression in tomato: A step towards CRISPR/Cas9-based genetic improvement.

Tomato (Solanum lycopersicum L.) is an essential source of antioxidants and a prime candidate for bioengineering experiments. Many studies have aimed to improve tomatoes using CRISPR/Cas9 technology; however, the success rate is limited due to the lack of efficient regeneration and genetic modification techniques.

Tracing the intraspecies expansion of glyoxalase genes and their expanding roles across the genus Oryza.

The genus Oryza is of utmost importance to human civilization as two of its species became agronomically productive and widely cultivated, and also because wild rice is a treasure trove of beneficial alleles that can be used for crop improvement. Most of the wild rice genotypes are known for their stress tolerance several times more than the domesticated rice varieties. In this study, we aimed to carry out an exhaustive genomic survey to identify glyoxalase I (GLYI) and glyoxalase II (GLYII) genes across the 11 rice genomes sequenced so far.

Introduction of dengue virus serotype 3 in the Afar Region, Ethiopia.

The genetic analysis of the Dengue virus circulating in Ethiopia's Afar region, in 2023, identified three distinct introductions with spatiotemporal clustering linked to genomes from Asia and Italy. These findings are crucial for enhancing prevention and control strategies, reinforcing the necessity to provide sustainable tools for genomic epidemiology in Africa.

The Genomic SSR Millets Database (GSMDB): enhancing genetic resources for sustainable agriculture.

The global population surge demands increased food production and nutrient-rich options to combat rising food insecurity. Climate-resilient crops are vital, with millets emerging as superfoods due to nutritional richness and stress tolerance. Given limited genomic information, a comprehensive genetic resource is crucial to advance millet research.

Enhanced lipid accumulation in microalgae Scenedesmus sp. under nitrogen limitation.

Microalgae-based biofuel production is cost-effective only in a biorefinery, where valuable co-products offset high costs. Fatty acids produced by photosynthetic microalgae can serve as raw materials for bioenergy and pharmaceuticals. This study aims to understand the metabolic imprints of Scenedesmus sp.

Epigenetic processes in insect adaptation to environmental stress.

Insects exhibit remarkable adaptability to a wide range of environmental stressors, including temperature fluctuations, pathogens, and changes in diet. This adaptability is often driven by epigenetic processes, which regulate gene expression without altering the underlying DNA sequence. This review provides a comprehensive overview of these epigenetic processes in insect adaptation, highlighting their impact on development, behaviour, and stress resilience.

The divergent outcome of IL-4Rα signalling on Foxp3 T regulatory cells in listeriosis and tuberculosis.

Introduction: Forkhead box P3 (Foxp3) T regulatory cells are critical for maintaining self-tolerance, immune homeostasis, and regulating the immune system.

Methods: We investigated interleukin-4 receptor alpha (IL-4Rα) signalling on T regulatory cells (Tregs) during () infection using a mouse model on a BALB/c background, specifically with IL-4Rα knockdown in Tregs (Foxp3IL-4Rα).

Results: We showed an impairment of Treg responses, along with a decreased bacterial burden and diminished tissue pathology in the liver and spleen, which translated into better survival.

Axon guidance genes are regulated by TDP-43 and RGNEF through long-intron removal.

Rho guanine nucleotide exchange factor (RGNEF) is a guanine nucleotide exchange factor (GEF) mainly involved in regulating the activity of Rho-family GTPases. It is a bi-functional protein, acting both as a guanine exchange factor and as an RNA-binding protein. RGNEF is known to act as a destabilizing factor of neurofilament light chain RNA (NEFL) and it could potentially contribute to their sequestration in nuclear cytoplasmic inclusions.

Lung damage in SARS-CoV-2 patients: An autopsy study in the era of vaccination.

Aims: The contribution of SARS-CoV-2 infection on lung damage and the effect of vaccination on either containing the number of deaths or mitigating lung damage has not been systematically investigated.

Methods: Post-mortem analysis was performed among consecutive in-patients with COVID-19 deceased in the Province of Trieste (2020-2022). The outcomes of the study were (i) rates of in-hospital mortality, (ii) contribution of COVID-19 to death, (iii) histological extent of lung injury and (iv) impact of vaccination.

Sub-acute bisphenol A exposure induces proteomic alterations and impairs male reproductive health in mice.

Bisphenol A (BPA) is one of the most prevalent endocrine disrupting chemicals (EDCs) and there is widespread concern about the adverse effects of EDCs on human health. However, the exact mechanism of these toxicities has still not been fully deciphered. Additionally, studies have reported the toxicological effects at far low doses to the generally considered no-observed-adverse-effect level (NOAEL) dose.