Effects of epigallocatechin gallate on the development of matrix-rich biofilm.

In this study, we evaluated the impact of Epigalocatechin-3-gallate (EGCG) on biofilm development for 24 and 46 h using high-resolution confocal laser scanning microscopy. EGCG treatment led to the formation of interspaced exopolysaccharide (EPS)-microcolony complexes unevenly distributed on the surface of hydroxyapatite disc, forming a thinner and less complex biofilm structure with significantly reduced biomass, matrix volume, and thickness compared to the NaCl treated group (negative control). At 46 h, the biofilm of the EGCG-treatment group failed to form the bacterial-EPS superstructures which is characteristic of the biofilm in the negative control group.

Changes in Occlusal Contacts upon the Cementation of Zirconia Crowns with Different Cement Spacers.

Occlusion plays a crucial role in the long-term success and functionality of dental restorations. The purpose of this study was to investigate the changes in occlusal contacts upon the cementation of zirconia crowns with different cement spacer settings in computer-aided design and computer-aided manufacturing (CAD-CAM) software (3Shape Dental System version 2.102.

sp. nov., isolated from a human colon tumor adheres to colonic epithelial cells and induces IL-8 secretion.

Cancerous tissue is a largely unexplored microbial niche that provides a unique environment for the colonization and growth of specific bacterial communities, and with it, the opportunity to identify novel bacterial species. Here, we report distinct features of a novel species, sp. nov.

Dental Clinic Deserts in the US: Spatial Accessibility Analysis.

Importance: Little is known about the spatial accessibility to dental clinics across the US.

Objective: To map the spatial accessibility of dental clinics nationally and to examine the characteristics of counties and US Census block groups with dental care shortage areas.

Design, Setting, And Participants: Cross-sectional study of US dental clinics in 2023 using data from the IQVIA national practitioners' database, which includes 205 762 active dentists.

Effects of aqueous and ethanolic extracts of Chinese propolis on dental pulp stem cell viability, migration and cytokine expression.

Background: Propolis is a natural substance produced by honeybees that has various biological properties including, anti-inflammatory, antioxidant and antimicrobial properties. Although previous studies have evaluated the antimicrobial effects of propolis in dentistry, its effects on dental pulp stem cell (DPSC) viability, migration, and differentiation are yet not well understood. The objective of this study was to investigate the effects of Chinese propolis on viability/proliferation, migration, differentiation and cytokine expression in DPSCs.

Identifying Pain Subtypes in Patients With Craniofacial Lesions of Fibrous Dysplasia/McCune-Albright Syndrome.

Background: Fibrous dysplasia/McCune-Albright syndrome (FD/MAS) is a genetic disorder, marked by bone lesions, often affecting the craniofacial skeleton. Pain is a prevalent yet heterogeneous symptom reported by patients with craniofacial FD. Effective treatments are currently lacking, posing a significant clinical challenge to patient care.

Digital trifunctional intraoral appliance for centric relation recording: A dental technique.

A technique for fabricating a trifunctional intraoral appliance is described. This appliance streamlines the process of recording centric relation by combining the functions of an anterior deprogrammer, gothic arch tracer, and record base with occlusal rims into a single device. The use of digital tools in this technique offers greater convenience and efficiency compared with conventional methods.

Medical malpractice court cases involving treatment of the temporomandibular joint: analysis of prevalence, risk factors, and court rulings.

Objective: Limited research exists regarding malpractice in dentistry. Temporomandibular joint disorders (TMDs) include intra- and extra-articular conditions that are managed by general dentists, orofacial pain specialists, and oral and maxillofacial surgeons. In this study, we investigate the rate of malpractice court trials involving treatment of TMD by these specialists.

Canada's First National Oral Health Research Strategy (2024-2030).

Recent years have seen significant positive changes and developments in oral health-related policy and data on oral health and oral health care in Canada. Simultaneously, on the international stage, the momentum for oral health and related research continues to build. These changes have led to an initiative to create Canada's first National Oral Health Research Strategy (NOHRS), which was recently published by the Canadian Institutes of Health Research-Institute of Musculoskeletal Health and Arthritis (Allison and Rock 2024).

Investigating the Role of Primary Cilia and Bone Morphogenetic Protein Signaling in Periodontal Ligament Response to Orthodontic Strain In Vivo and In Vitro: A Pilot Study.

Periodontal ligament (PDL) cells are crucial for mechanosensation and mechanotransduction within the PDL, yet the role of primary cilia in orthodontic force transmission has not been examined. While bone morphogenetic protein (BMP) signaling significantly influences ciliary function, its effect on cellular responses to mechanical stress has not been investigated. This study aims to investigate whether primary cilia and BMP signaling are involved in the periodontal ligament's response to orthodontic tooth movement and the resultant mechanical strain.

Innovative Orthodontic-Restorative Treatment With Customized CAD/CAM Smartwires.

Background: The demand for discreet and low-compliance appliances has driven innovation in orthodontics, particularly with technological advances in artificial intelligence, robotics, and CAD/CAM technology. The evolution of Programmed Non-Sliding Mechanics for precise, automated tooth movement is the latest innovation.

Aim: In this article, we aimed to demonstrate the application of a novel orthodontic lingual appliance, The Gen 2 InBrace system (InBrace, Irvine, CA), in the orthodontic-restorative treatment of an adult patient with anterior tooth-size discrepancies, anterior openbite, and incisor proclination.

The Impact of Membrane Stabilization for Alveolar Ridge Preservation in Periodontally Compromised Extraction Sockets: A Non-Inferiority Randomized Controlled Trial.

Aim: The aim of this study was to investigate the non-inferiority of alveolar ridge preservation (ARP) with membrane stabilization compared with non-stabilization, focusing on changes of soft-tissue contour in periodontally compromised extraction sockets. Secondary outcomes included changes in hard-tissue contour, patient-reported outcomes and new bone formation.

Materials And Methods: Twenty-four patients with periodontally compromised teeth were randomly assigned to ARP with (test group) or without (control group) membrane stabilization.

Effect of CAD/CAM Abutment Morphology on the Outcomes of Implant Therapy: A Randomized Controlled Trial.

Objectives: Variations in transmucosal abutment contour design may affect the outcomes of implant therapy. This randomized controlled trial was primarily aimed at testing the effect that CAD/CAM zirconia abutments with either a concave or linear divergent transmucosal morphology have on peri-implant mucosal dynamics and indicators of peri-implant health at 1 year after final implant-supported prosthesis insertion in the anterior maxilla.

Materials And Methods: Following computer-guided implant placement and osseointegration, eligible subjects were randomized into either the experimental (concave morphology) or the control (linear divergent morphology) group.

A clinical comparative analysis using an optical tracking device versus conventional tracking device in the production of occlusal appliances.

Purpose: Optical tracking devices (OTDs) hold promise for enhancing patient-centered prostheses, but their efficacy remains underexplored. This clinical study aimed to comprehensively evaluate differences in static and dynamic occlusions among occlusal appliances fabricated using optical tracking, conventional tracking, and average articulator values (AAVs), providing insights into their efficacy in clinical and research-based practices.

Materials And Methods: Twelve dentate participants aged over 25 years, with Angle Class I and II occlusal relationships, were enrolled.

DNA and RNA Methylation in Periodontal and Peri-implant Diseases.

Periodontal and peri-implant diseases are primarily biofilm-induced pathologies in susceptible hosts affecting the periodontium and dental implants. Differences in disease susceptibility, severity, and patterns of progression have been attributed to immune regulatory mechanisms such as epigenetics. DNA methylation is an essential epigenetic mechanism governing gene expression that plays pivotal roles in genomic imprinting, chromosomal stability, apoptosis, and aging.

The Influence of Abutment Selection in the Tridimensional Implant Position: Considerations for Predictable Implant Restorative Long-Term Outcomes.

Objective: To explore the influence of abutment selection on 3D implant positioning, emphasizing the synergy between surgical and prosthetic considerations for achieving predictable long-term outcomes in implant-supported restorations.

Main Considerations: Implant dentistry has transitioned from a purely surgical approach to a prosthetically driven methodology that prioritizes implant-supported restoration (ISR). This shift has been bolstered by advancements in digital technologies and abutment designs, which allow for more precise implant positioning and better management of biological, mechanical, and esthetic outcomes.

Every bite counts to achieve oral health: a scoping review on diet and oral health preventive practices.

Objective: To examine the landscape of preventive strategies and interventions directed to achieve oral health equity, with particular emphasis on the interplay between dental caries prevention, individual behaviors, and population-level strategies across various demographic and geographic regions.

Methods: This scoping review was guided by Peters et al.'s framework, which incorporates four key concepts aimed at reducing caries: education for individuals and healthcare providers, behavioral modifications, addressing broader social determinants of health, and extending oral health education programs beyond traditional dental settings.

Latest update on the use of recombinant growth factors for periodontal regeneration: existing evidence and clinical applications.

Growth factors were introduced to increase predictability in periodontal regeneration and have since been widely applied in dentistry. This narrative review article highlights histological and latest findings of recombinant human platelet-derived growth factor-BB (rhPDGF-BB) and recombinant human fibroblast growth factor-2 (rhFGF-2) for periodontal regeneration. rhPDGF-BB enhances the proliferation and chemotaxis of periodontal ligament and alveolar bone cells.