Correlation Between Ki-67 Expression and Tumor Grade in Breast Cancer: A Cross-Sectional Study.

Introduction Ki-67 is a proliferation marker that may correlate with tumor grade in breast cancer. This study explores the association between Ki-67 expression levels and tumor grade in breast cancer patients. Materials and methods This cross-sectional study analyzed Ki-67 expression in tumor samples from 280 breast cancer patients.

Association Between the Treatment Modality of Pediatric Subcondylar Fractures and Functional Outcomes at the Six-Month Follow-Up: A Retrospective Pilot Study.

Background: The choice of treatment for subcondylar fractures in children and adolescents remains a controversial issue. The aim of this study was to evaluate the association between the treatment modality of subcondylar fractures and functional outcomes at the six-month follow-up.

Methods: This retrospective study examined a cohort of children and adolescents with unilateral or bilateral subcondylar fractures treated at a level 1 trauma center over a five-year period.

Perceptions of Orthopedic and Musculoskeletal Practice Among Primary Care Physicians in Japan: A Questionnaire Survey Analysis of Jichi Medical University Graduates.

Background Primary care physicians (PCPs) are expected to engage in comprehensive medical care, including orthopedic and musculoskeletal problems. This study aimed to assess perceptions and current status regarding orthopedic and musculoskeletal practice among PCPs in Japan. Methodology A cross-sectional survey was conducted among PCPs who graduated from Jichi Medical University (JMU) with opportunities to treat orthopedic and musculoskeletal problems.

Elucidating the dual roles of apoptosis and necroptosis in diabetic wound healing: implications for therapeutic intervention.

Wound healing is a complex and multistep biological process that involves the cooperation of various cell types. Programmed cell death, including apoptosis and necrotizing apoptosis, plays a crucial role in this process. Apoptosis, a controlled and orderly programmed cell death regulated by genes, helps eliminate unnecessary or abnormal cells and maintain internal environmental stability.

Exercise therapy facilitates neural remodeling and functional recovery post-spinal cord injury via PKA/CREB signaling pathway modulation in rats.

Background: Neuronal structure is disrupted after spinal cord injury (SCI), causing functional impairment. The effectiveness of exercise therapy (ET) in clinical settings for nerve remodeling post-SCI and its underlying mechanisms remain unclear. This study aims to explore the effects and related mechanisms of ET on nerve remodeling in SCI rats.

Multi-omics perspective: mechanisms of gastrointestinal injury repair.

In this review, we examine the significance of multi-omics technologies in understanding the plethora of intricate processes that activate gastrointestinal (GI) injury repair. Multi-omics, which includes genomics, transcriptomics, proteomics, and metabolomics, allows intricate mapping of cellular responses and molecular pathways involved in GI repair. We highlight the potential of multi-omics to discover previously unknown therapeutic targets or elucidate the molecular basis of the pathogenesis of GI.

Integration of single-cell transcriptomics and bulk transcriptomics to explore prognostic and immunotherapeutic characteristics of nucleotide metabolism in lung adenocarcinoma.

Background: Lung adenocarcinoma (LUAD) is a highly aggressive tumor with one of the highest morbidity and mortality rates in the world. Nucleotide metabolic processes are critical for cancer development, progression, and alteration of the tumor microenvironment. However, the effect of nucleotide metabolism on LUAD remains to be thoroughly investigated.

DLBWE-Cys: a deep-learning-based tool for identifying cysteine S-carboxyethylation sites using binary-weight encoding.

Cysteine S-carboxyethylation, a novel post-translational modification (PTM), plays a critical role in the pathogenesis of autoimmune diseases, particularly ankylosing spondylitis. Accurate identification of S-carboxyethylation modification sites is essential for elucidating their functional mechanisms. Unfortunately, there are currently no computational tools that can accurately predict these sites, posing a significant challenge to this area of research.

SidF, a dual substrate N5-acetyl-N5-hydroxy-L-ornithine transacetylase involved in siderophore biosynthesis.

Siderophore-mediated iron acquisition is essential for the virulence of , a fungus causing life-threatening aspergillosis. Drugs targeting the siderophore biosynthetic pathway could help improve disease management. The transacetylases SidF and SidL generate intermediates for different siderophores in .

Huanglian Ejiao Decoction Alleviates Ulcerative Colitis in Mice Through Regulating the Gut Microbiota and Inhibiting the Ratio of Th1 and Th2 Cells.

Background: Huanglian-ejiao decoction (HED) is a Chinese traditional medicinal formula evolved from the Shanghan Lun (Treatise on Febrile Diseases). However, HED ultimate mechanism of action remained indistinct. Therefore, this study aimed to investigate whether HED could exert anti-inflammatory effects on 2,4,6-Trinitrobenzenesulfonic acid (TNBS)-induced colitis (UC) model through the regulation of CD4T subsets and gut microbiota.

Injectable microspheres filled with copper-containing bioactive glass improve articular cartilage healing by regulating inflammation and recruiting stem cells.

Osteoarthritis (OA) is a frequent chronic illness in orthopedics that poses a major hazard to patient health. cell therapy is emerging as a therapeutic option, but its efficacy is influenced by both the inflammatory milieu and the amount of stem cells, limiting its use. In this study, we designed a novel injectable porous microsphere (PM) based on microfluidic technology that can support mesenchymal stem cells (MSCs) therapy by combining polylactic-glycolic acid copolymer, kartogenin, polydopamine, stromal cell-derived factor-1, and copper-doped bioactive glass (CuBG).

Application progress of artificial intelligence in tumor diagnosis and treatment.

The rapid advancement of artificial intelligence (AI) has introduced transformative opportunities in oncology, enhancing the precision and efficiency of tumor diagnosis and treatment. This review examines recent advancements in AI applications across tumor imaging diagnostics, pathological analysis, and treatment optimization, with a particular focus on breast cancer, lung cancer, and liver cancer. By synthesizing findings from peer-reviewed studies published over the past decade, this paper analyzes the role of AI in enhancing diagnostic accuracy, streamlining therapeutic decision-making, and personalizing treatment strategies.

SPEMix: a lightweight method via superclass pseudo-label and efficient mixup for echocardiogram view classification.

Introduction: In clinical, the echocardiogram is the most widely used for diagnosing heart diseases. Different heart diseases are diagnosed based on different views of the echocardiogram images, so efficient echocardiogram view classification can help cardiologists diagnose heart disease rapidly. Echocardiogram view classification is mainly divided into supervised and semi-supervised methods.

Efficacy of the Protocol for Trauma Team Activation in Taipei: A Retrospective Study.

Trauma triage is the use of trauma assessment for prioritizing patients for treatment or transport by injury severity. According to Taiwan Public Health Report, accidents and their adverse events were the sixth leading cause of death and accounted for over 7000 casualties in 2009. However, a lack of accuracy in identifying the severity of a patient's injury and their prehospital information can result in inappropriate triage.

Exploring the effect of gestational diabetes mellitus on retinal vascular morphology by PKSEA-Net.

Background: Gestational diabetes mellitus (GDM) is a temporary metabolic disorder in which small retinal vessels may have experience subtle changes before clinical lesions of the fundus retina appear. An innovative artificial intelligence image processing technology was applied to locate and analyze the small retinal vessel morphology and accurately evaluate the changes of the small retinal vessels in GDM patients and pregnant women with normal blood glucose and non-pregnant women with normal blood glucose.

Methods: The subjects were divided into three groups:GDM group, pregnant control group (PC), and normal control group (NC).

A scATAC-seq atlas of stasis zone in rat skin burn injury wound process.

Burn injuries often leave behind a "stasis zone", a region of tissue critically important for determining both the severity of the injury and the potential for recovery. To understand the intricate cellular and epigenetic changes occurring within this critical zone, we utilized single-cell assay for transposase-accessible chromatin sequencing (scATAC-seq) to profile over 31,500 cells from both healthy rat skin and the stasis zone at nine different time points after a burn injury. This comprehensive approach revealed 26 distinct cell types and the dynamic shifts in the proportions of these cell types over time.

A multi-modal multi-branch framework for retinal vessel segmentation using ultra-widefield fundus photographs.

Background: Vessel segmentation in fundus photography has become a cornerstone technique for disease analysis. Within this field, Ultra-WideField (UWF) fundus images offer distinct advantages, including an expansive imaging range, detailed lesion data, and minimal adverse effects. However, the high resolution and low contrast inherent to UWF fundus images present significant challenges for accurate segmentation using deep learning methods, thereby complicating disease analysis in this context.

Evaluation of autophagic and apoptotic markers during infection with animal virus causing hemorrhagic fever in rabbits.

Introduction: /GI.1 and GI.2 cause severe Rabbit Haemorrhagic Disease, and immune processes are among the important pathomechanisms of the disease.

Revolutionizing diagnosis of pulmonary based on CT: a systematic review of imaging analysis through deep learning.

Introduction: The mortality rate associated with (MTB) has seen a significant rise in regions heavily affected by the disease over the past few decades. The traditional methods for diagnosing and differentiating tuberculosis (TB) remain thorny issues, particularly in areas with a high TB epidemic and inadequate resources. Processing numerous images can be time-consuming and tedious.

Novel target and cofactor repertoire for the transcriptional regulator JTY_0672 from BCG.

(Mtb) is the pathogenic agent of tuberculosis (TB). Intracellular survival plays a central role in the pathogenesis of Mtb in a manner that is dependent on an array of transcriptional regulators for Mtb. However, the functionality of JTY_0672, a member of the TetR family of transcriptional regulators, remains unknown.

Comparison of the efficacy of LTCBDE and LCBDE for common bile duct stones: a systematic review and meta-analysis.

Background: The choice of surgical methods for common bile duct stones (CBDS) is controversial. The aim of this study was to compare the safety and efficacy of laparoscopic transcystic common bile duct exploration (LTCBDE) and laparoscopic common bile duct exploration (LCBDE).

Methods: Relevant literature published before March 30, 2023 in PubMed, Web of Science, Embase, and Cochrane was searched to screen studies comparing LTCBDE and LCBDE.

Exploring the Kidney-Brain Crosstalk: Biomarkers for Early Detection of Kidney Injury-Related Alzheimer's Disease.

Background: The phenomenon of "kidney-brain crosstalk" has stimulated scholarly inquiry into the correlations between kidney injury (KI) and Alzheimer's disease (AD). Nonetheless, the precise interactions and shared mechanisms between KI and AD have yet to be fully investigated. The primary goal of this study was to investigate the link between KI and AD, with a specific focus on identifying diagnostic biomarkers for KI-related AD.

A Proteomic Study Based on Home Quarantine Model Identifies NQO1 and Inflammation Pathways Involved in Adenoid Hypertrophy.

Background: Adenoid hypertrophy is a common disorder of childhood, and has an unclear pathogenesis. At the beginning of the COVID-19 pandemic, there was a significant reduction in the incidence of adenoid hypertrophy in children under long-term home quarantine, providing a rare research model to explore the pathogenesis and treatment targets of adenoidal hypertrophy in children.

Methodology: Before and during the home quarantine period, adenoids that underwent surgery were detected using label-free proteomics.

The Clinical Value of the Combined Detection of Systemic Immune-Inflammation Index (SII), Systemic Inflammation Response Index (SIRI), and Prognostic Nutritional Index (PNI) in Early Diagnosis of Gastric Cancer.

Objective: Gastric cancer (GC) is a common malignant tumor of the digestive tract. Accumulating studies suggest that inflammation is linked with the pathogenesis of GC. The study delves into novel hematological inflammatory markers, such as systemic immune-inflammation index (SII), systemic inflammation response index (SIRI), and prognostic nutritional index (PNI), to explore their potential applications in early diagnosis of GC.

New Insights into the Role of Inflammatory Pathways and Immune Cell Infiltration in Sleep Deprivation-Induced Atrial Fibrillation: An Integrated Bioinformatics and Experimental Study.

Background: The common occurrence of atrial fibrillation (AF) as a cardiac arrhythmia, along with its link to sleep deprivation (SD), is gaining more acknowledgment. Even with progress in comprehending the development of AF, the molecular connections between SD and AF are still not well-defined. The objective of this research was to pinpoint the shared molecular routes responsible for SD-induced AF and investigate possible treatment targets.