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Early ultrastructural damage in retina and optic nerve following intraocular pressure elevation.
Vision Res
January 2025
Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
Elevated intraocular pressure (IOP) is a significant risk factor for glaucoma, causing structural and functional damage to the eye. Increased IOP compromises the metabolic and structural integrity of retinal ganglion cell (RGC) axons, leading to progressive degeneration and influencing the ocular immune response. This study investigated early cellular and molecular changes in the retina and optic nerve (ON) following ocular hypertension (OHT).
View Article and Find Full Text PDFFatty acid methyl ester (FAME) profiling for species-specific characterization and detection of fungal pathogens that cause tree and grapevine trunk diseases.
Mycologia
January 2025
Crops Pathology and Genetics Research Unit, USDA-ARS Davis, Davis, California 95616.
Fungal trunk diseases are of major concern for tree fruit, nut, and grape growers throughout the world. These diseases include Eutypa dieback of grape, caused by , band canker of almond, caused by and , and twig and branch dieback of walnut, caused by , Botryosphaeria dieback of grape, caused by , and , and esca of grape, caused by and . Given the common occurrence of mixed infections, and the similar wood symptoms at the macroscopic level, species-specific detection tools are needed.
View Article and Find Full Text PDFMethyl Syringate Monoglucoside Is a Crucial Intermediate in Leptosperin Biosynthesis in (ma̅nuka).
J Agric Food Chem
January 2025
Faculty of Health and Nutrition, Shubun University, Ichinomiya, Aichi 491-0938, Japan.
Leptosperin (methyl syringate-4--β-d-gentiobioside) serves as a unique marker for ma̅nuka honey, derived from the ma̅nuka plant (). Despite its importance, the biosynthesis pathway of leptosperin remains unreported. This study investigates the molecular mechanism of leptosperin formation from its aglycone, methyl syringate (MSYR), in ma̅nuka plants.
View Article and Find Full Text PDFPolyfluorene-Enhanced Near-Infrared Electrochemiluminescence of Heptamethine Cyanine Dye for Coreactants-Free Bioanalysis.
Anal Chem
January 2025
Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
The near-infrared electrochemiluminescence (NIR-ECL) technique has received special attention in cell imaging and biomedical analysis due to its deep tissue penetration, low background interference, and high sensitivity. Although cyanine-based dyes are promising NIR-ECL luminophores, limited ECL efficiency and the need for exogenous coreactants have prevented their widespread application. In this work, poly[9,9-bis(3'-(-dimethylamino)propyl)-2,7-fluorene]--2,7-(9,9-dioctylfluorene)] (PFN) was innovatively developed to significantly invigorate the NIR-ECL performance of heptamethine cyanine dye IR 783 by the resonance energy transfer (RET) strategy.
View Article and Find Full Text PDFDynamic molecular architecture of the synaptonemal complex.
Sci Adv
January 2025
Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720-3200, USA.
During meiosis, pairing between homologous chromosomes is stabilized by the assembly of the synaptonemal complex (SC). The SC ensures the formation of crossovers between homologous chromosomes and regulates their distribution. However, how the SC regulates crossover formation remains elusive.
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