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Hyperspectral Imaging for High Throughput Optical Spectroscopy of pL Droplets.
Anal Chem
January 2025
Nanophotonic Systems Laboratory, Department of Mechanical and Process Engineering, ETH Zurich, 8092 Zurich, Switzerland.
Droplet-based microfluidics is a powerful tool for high-throughput analysis of liquid samples with significant applications in biomedicine and biochemistry. Nevertheless, extracting content-rich information from single picolitre-sized droplets at high throughputs remains challenging due to the weak signals associated with these small volumes. Overcoming this limitation would be transformative for fields that rely on high-throughput screening, enabling broader multiparametric analysis.
View Article and Find Full Text PDFThis paper introduces an interferometer for single-shot areal quantitative phase imaging at two wavelengths simultaneously, suitable for use with low coherence sources. It operates in reflection geometry with on-axis illumination, so that it can be conveniently applied to surface texture measurements. The system consists of two identical 4f systems forming the reference and sample arm.
View Article and Find Full Text PDFWe demonstrate PaCMAN, a ptychography algorithm that can reconstruct high quality images with broadband illumination sources while being robust to shot, detector, and parasitic noise. We extend prior monochromatization work to improve accuracy, especially for discrete spectra, and also demonstrate how PaCMAN can be converted into Ms. PaCMAN, a multi-spectral variant that outperforms multi-spectral ePIE.
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View Article and Find Full Text PDFClassical and quantum states working in concert play an essential role in high-precision interferometry. In this regard, coherent combined with squeezed vacuum states are the most promising candidate. Here we complement this subject by comparing nonlinear and nonlinear-linear hybrid interferometers with homodyne detection as a readout strategy.
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