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The potential function of chalcone isomerase (CHI) gene on flavonoid accumulation in Amomum tsao-ko fruit by transcriptome and metabolome.
Int J Biol Macromol
January 2025
Key Laboratory for Research and Utilization of Characteristic Biological Resources in Southern Yunnan, College of Biological and Agricultural Sciences, Honghe University, Mengzi 661199, Yunnan, China. Electronic address:
Flavonoids are the major medicinally active ingredients that exert potential effects in Amomum tsao-ko. In total, 277 flavonoid metabolites were identified in fresh and dried fruits of three different accessions of A. tsao-ko (Amomum tsao-ko), which could be classified into eight classes with more metabolites classified as flavonol.
View Article and Find Full Text PDFStructural characterization of complex tannins from Euryale ferox fruit peels and their inhibitory mechanisms against tyrosinase activity and melanogenesis.
Int J Biol Macromol
January 2025
College of Life Science, Yangtze University, Jingzhou, China. Electronic address:
Tyrosinase is a rate-limiting enzyme for melanogenesis and abnormal melanin production can be controlled by utilizing tyrosinase inhibitory substances. To develop potent and safe inhibitors of tyrosinase, complex tannins a narrowly distributed plant polyphenols were prepared from the fruit peel of Euryale ferox (EPTs) and then structurally characterized, as well as investigated for their inhibitory effects and the involved mechanisms against tyrosinase activity and melanogenesis. The structures of EPTs were established to consist of 63.
View Article and Find Full Text PDFPolysaccharides isolated from Cibotium barometz attenuate chronic inflammatory pain: Molecular chemical structure and role of phenylalanine.
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Department of Pain Management, Qilu Hospital of Shandong University, 107# West Wenhua Road, Jinan, Shandong 250012, China. Electronic address:
This investigation represents a pioneering effort to examine the therapeutic effects of PCB specifically in the context of CFA-induced mice, as well as to elucidate the underlying mechanisms that facilitate such effects. Our study utilized advanced methodologies, namely high-performance liquid chromatography coupled with mass spectrometry (HPLC-MS)-based metabolomics, alongside comprehensive multivariate data analysis, to identify a distinctive metabolic profile associated with acute inflammation. Through our analyses, we discovered that several potential metabolites were significantly implicated in a variety of critical metabolic pathways.
View Article and Find Full Text PDFConstruction of a fusant bacterial strain simultaneously degrading atrazine and acetochlor and its application in soil bioremediation.
Sci Total Environ
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Jilin Academy of Agricultural Sciences (Northeast Agricultural Research Center of Chin), Gongzhuling 136100, Jilin, China. Electronic address:
Application of herbicide-degrading bacteria is an effective strategy to remove herbicide in soil. However, the ability of bacteria to degrade a herbicide is often severely limited in the presence of other pesticide. In this study, the atrazine-degrading strain Klebsiella varicola FH-1 and acetochlor-degrading strain Bacillus Aryabhatti LY-4 were used as parent strains to construct the recombinant RH-92 strain through protoplast fusion technology.
View Article and Find Full Text PDFCrystal structure of the anti-CRISPR protein AcrIE7.
Biochem Biophys Res Commun
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Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, 300070, China; Department of Pharmacology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, 300070, China. Electronic address:
Bacterial adaptive immunity, driven by CRISPR-Cas systems, protects against foreign nucleic acids from mobile genetic elements (MGEs), like bacteriophages. The type I-E CRISPR-Cas system employs the Cascade (CRISPR-associated complex for antiviral defense) complex for target DNA cleavage, guided by crRNA. Anti-CRISPR (Acr) proteins, such as AcrIE7, counteract this defense by inhibiting Cascade activity.
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