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A bioluminescent reporter bioassay for in-process assessment of chimeric antigen receptor lentiviral vector potency.
Antib Ther
January 2025
Research and Development, Promega Corp., Madison, WI 53711, United States.
Background: Chimeric antigen receptor (CAR)-T-cell therapy is a breakthrough in the field of cancer immunotherapy, wherein T cells are genetically modified to recognize and attack cancer cells. Delivery of the CAR gene is a critical step in this therapy and is usually achieved by transducing patient T cells with a lentiviral vector (LV). Because the LV is an essential component of CAR-T manufacturing, there is a need for simple bioassays that reflect the mechanism of action (MOA) of the LV and can measure LV potency with accuracy and specificity.
View Article and Find Full Text PDFTexture analysis combined with machine learning in radiographs of the knee joint: potential to identify tibial plateau occult fractures.
Quant Imaging Med Surg
January 2025
School of Management and Economics, University of Electronic Science and Technology of China, Chengdu, China.
Background: Missed or delayed diagnosis of occult fractures of tibial plateau may cause adverse effects on patients. The objective of this study was to evaluate the diagnostic performance of texture analysis (TA) of knee joint radiographs combined with machine learning (ML) in identifying patients at risk of tibial plateau occult fractures.
Methods: A total of 169 patients with negative fracture on knee X-ray films from 2018 to 2022 who were diagnosed with occult tibial plateau fractures or no fractures by subsequent magnetic resonance imaging (MRI) examination were retrospectively enrolled.
[Surveillance of the population density of adult in Guangdong Province from 2018 to 2023].
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi
August 2024
Institute of Disinfection and Vector Control, Guangdong Provincial Center for Disease Control and Prevention, Guangzhou, Guangdong 511430, China.
Objective: To investigate the fluctuations in the population density of and changes in the population density of in different geographical areas and different breeding habitats in Guangdong Province from 2018 to 2023, so as to provide insights into prevention and control of mosquito-borne infectious diseases in the province.
Methods: surveillance sites were assigned in 1 609 townships (streets) from 121 districts (counties) of 21 cities in Guangdong Province during the period between March and November from 2018 to 2023. The surveillance of the population density of was performed once a month in each surveillance site, and once a month in specific settings in cities where dengue were highly prevalent in Guangdong Province from December to February of the next year during the period from 2018 through 2023.
[Epidemiological and spatial distribution characteristics of human infections in Guangdong Province from 2016 to 2022].
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi
October 2024
Guangdong Provincial Center for Disease Control and Prevention; Guangdong Workstation for Emerging Infectious Disease Control and Prevention, Guangzhou, Guangdong 511430, China.
Objective: To investigate the epidemiological characteristics and spatial distribution characteristics of human infections in Guangdong Province from 2016 to 2022, so as to provide insights into formulation of the clonorchiasis control measures in the province.
Methods: Xinhui District of Jiangmen City, Longmen County of Huizhou City and Wengyuan County of Shaoguan City in Guangdong Province were selected as fixed surveillance sites for human clonorchiasis from 2016 to 2022, and additional 10% to 15% counties (districts) endemic for clonorchiasis were sampled from Guangdong Province as mobile surveillance sites each year from 2016 to 2022. A village (community) was randomly selected from each surveillance site according to the geographical orientations of east, west, south, north and middle, and subjects were randomly sampled from each village (community).
Comparative evaluation of several toric intraocular lens calculators for predicting postoperative refractive astigmatism in cataract surgery: a real-world study.
BMC Ophthalmol
January 2025
Department of Ophthalmology, Peking University Third Hospital, 49 North Garden Rd., Haidian District, Beijing, China.
Background: This study aims to comprehensively evaluate the predictive accuracy of six widely used toric intraocular lens (IOL) calculators in eyes undergoing cataract surgery with toric IOL implantation.
Methods: This retrospective study reviewed 53 eyes of 53 patients that underwent cataract extraction with toric IOL implantation using Zeiss 709 M. Six toric IOL calculators were evaluated: Barrett toric calculator (with predicted PCA, measured PCA, and TK), Kane formula (predicted PCA), and EVO 2.
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