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Engineering thermostability of industrial enzymes for enhanced application performance.
Int J Biol Macromol
December 2024
Engineering Research Center of Ministry of Education on Food Synthetic Biotechnology and School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, China; Science Center for Future Foods, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, China. Electronic address:
Thermostability is a key factor for the industrial application of enzymes. This review categorizes enzymes by their applications and discusses the importance of engineering thermostability for practical use. It summarizes fundamental theories and recent advancements in enzyme thermostability modification, including directed evolution, semi-rational design, and rational design.
View Article and Find Full Text PDFVacuum suction catheter semi-rigid ureteroscopic laser lithotripsy for impacted upper ureteral stones: randomised controlled trial.
Int J Surg
December 2024
Department of Urology, Zhongnan Hospital of Wuhan University, Wuhan, China.
Purpose: To evaluate the effectiveness and safety of an upgraded integrated vacuum suction catheter in semi-rigid ureteroscopic laser lithotripsy (VC-URSL) compared to traditional methods for treating impacted upper ureteral stones.
Patients And Methods: This prospective, randomized controlled trial was conducted from September 2022 to March 2024 at a single center, enrolling 95 patients aged 18 to 70 years with a single radiopaque impacted upper ureteral stone. Participants were randomized into two groups: the VC-URSL group used an integrated vacuum suction catheter featuring a stainless steel stabilizing tube and a narrowed distal end to prevent obstruction, while the T-URSL group underwent standard ureteroscopic lithotripsy without vacuum assistance.
Caterpillar-Inspired Multi-Gait Generation Method for Series-Parallel Hybrid Segmented Robot.
Biomimetics (Basel)
December 2024
College of Mechanical and Electrical Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China.
The body structures and motion stability of worm-like and snake-like robots have garnered significant research interest. Recently, innovative serial-parallel hybrid segmented robots have emerged as a fundamental platform for a wide range of motion modes. To address the hyper-redundancy characteristics of these hybrid structures, we propose a novel caterpillar-inspired Stable Segment Update (SSU) gait generation approach, establishing a unified framework for multi-segment robot gait generation.
View Article and Find Full Text PDFPEDOT: PSS-Modified Organic Flexible and Implantable Microelectrode for Internal Bi-Directional Electrophysiology of Three-Dimensional Cardiomyocyte Spheroid.
ACS Sens
December 2024
Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Education Ministry, Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, China.
Three-dimensional (3D) cardiomyocyte spheroids are essential models to replicate cardiac structural and functional features in vitro. However, conventional planar and rigid microelectrode arrays (MEAs) suffer from low-quality electrophysiological recording of 3D cultures, due to limited contact areas and weak coupling between cells and MEA chips. Herein, we developed a PEDOT: PSS-modified organic flexible and implantable MEA (OFI-MEA) coupled with a self-developed integrated biosensing platform to achieve high-throughput, long-term, and stable bidirectional internal electrophysiology in 3D cardiomyocyte spheroids.
View Article and Find Full Text PDFTuning Fluorescence Properties of BIM Probes via Clay Integration: Targeting Rifampicin with improved selectivity.
Chem Asian J
December 2024
Birla Institute of Technology & Science Pilani - Hyderabad Campus, Chemistry department, Shameerpet, 500078, Hyderabad, INDIA.
The incorporation of photoactive organic dyes into layered inorganic materials enhances their optical and chemical properties, making them ideal for sensing applications. In this study, Bisindolyl methane (BIM)-based neutral probes were integrated with bentonite clay to explore their sensing capabilities. Probe 1 (unoxidized BIM) and Probe 2 (oxidized BIM) generally exhibited quenched luminescence in solution due to intramolecular rotations.
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