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High-precision and non-invasive measurement of crestal bone level by optical coherence tomography.
J Dent Sci
January 2025
School of Dentistry, National Yang Ming Chiao Tung University, Taipei, Taiwan.
Background/purpose: Identifying crestal bone level (CBL) on the buccal and lingual aspects poses challenges in conventional dental radiographs. Given that optical coherence tomography (OCT) has the capability to non-invasively provide in-depth information about the periodontium, this in vitro study aimed to assess whether OCT can effectively identify periodontal landmarks and measure CBL in the presence of gingiva.
Materials And Methods: An in-house handheld scanning probe connected to a 1310-nm swept-source OCT (SS-OCT) system, along with self-developed algorithms were employed to measure the CBL in dental models with artificial gingiva.
Aim: Fixed retention is the method of choice for permanent stabilization of the treatment outcome. In recent years, CAD/CAM techniques have been developed to produce retainers with high precision and tension-free fit. The aim of this retrospective study was to evaluate the suitability of a semi-industrial retainer manufacturing process (office-based construction, external laboratory manufacturing) in terms of positioning accuracy and post-treatment changes.
View Article and Find Full Text PDFSurface-Sensitive Waveguide Imaging for In Situ Analysis of Membrane Protein Binding Kinetics.
Anal Chem
January 2025
Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
Ligand binding to membrane proteins initiates numerous therapeutic processes. Surface plasmon resonance (SPR), a popular method for analyzing molecular interactions, has emerged as a promising tool for in situ determination of membrane protein binding kinetics owing to its label-free detection, high surface sensitivity, and resistance to intracellular interference. However, the excitation of SPR relies on noble metal films, typically gold, which are biologically incompatible and can cause fluorescence quenching.
View Article and Find Full Text PDFTwo-Dimensional Liquid Chromatography-Isotope Ratio Mass Spectrometry Opens New Possibilities in the Field of Compound-Specific Stable Isotope Analysis.
Anal Chem
January 2025
Instrumental Analytical Chemistry, University of Duisburg-Essen, Universitätsstraße 5, 45141 Essen, Germany.
Compound-specific stable isotope analysis (CSIA) using liquid chromatography-isotope ratio mass spectrometry (LC-IRMS) is a powerful tool for determining the isotopic composition of carbon in analytes from complex mixtures. However, LC-IRMS methods are constrained to fully aqueous eluents. Previous efforts to overcome this limitation were unsuccessful, as the use of organic eluents in LC-IRMS was deemed impossible.
View Article and Find Full Text PDFEthiodized Oil Pickering Emulsion for Sustained Release of Anti-PD-L1 Antibodies.
J Vasc Interv Radiol
January 2025
Department of Diagnostic and Interventional Radiology, Osaka University Graduate School of Medicine. 2-2 Yamadaoka, Suita, Osaka, 565-0871, Japan.
Purpose: This research aimed to develop and assess a Lipiodol Pickering emulsion containing anti-Programmed cell Death Ligand 1 (PD-L1) antibodies through in vitro experiments.
Materials And Methods: The emulsion was created by combining Lipiodol with poly (lactic-co-glycolic acid) (PLGA) nanoparticles and anti-PD-L1 antibodies. Confocal laser microscopy was used to evaluate the encapsulation of the antibodies within the Pickering emulsion.
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