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Multi-culture label-free quantitative cell migration sensing with single-cell precision.
Biomed Opt Express
January 2025
Warsaw University of Technology, Institute of Micromechanics and Photonics, 8 Sw. A. Boboli St., 02-525 Warsaw, Poland.
A fair comparison of multiple live cell cultures requires examining them under identical environmental conditions, which can only be done accurately if all cells are prepared simultaneously and studied at the same time and place. This contribution introduces a multiplexed lensless digital holographic microscopy system (MLS), enabling synchronous, label-free, quantitative observation of multiple live cell cultures with single-cell precision. The innovation of this setup lies in its ability to robustly compare the behaviour, i.
View Article and Find Full Text PDFOptical tweezers in biomedical research - progress and techniques.
J Med Life
November 2024
Biophysics and Cellular Biotechnology Department, Carol Davila University of Medicine and Pharmacy, Bucharest, Romania.
Optical tweezers, which leverage the forces exerted by radiation pressure, have emerged as a pivotal technique for precisely manipulating and analyzing microscopic particles. Since Arthur Ashkin's ground-breaking work in the 1970s and the subsequent development of the single-beam optical trap in 1986, the capabilities of optical tweezers have expanded significantly, enabling the intricate manipulation of biological specimens at the micro- and nanoscale. This review elucidates the foundational principles of optical trapping and their extensive applications in the biomedical sciences.
View Article and Find Full Text PDFInvestigating the link between a layer thickness and surface relief depth in an azo poly(ether imide).
Sci Rep
January 2025
Faculty of Physics, Warsaw University of Technology, 75 Koszykowa Str., 00-662, Warszawa, Poland.
Surface relief grating formation in photo-responsive azo polymers under irradiation is a long-ago-found phenomenon, but all the factors governing its efficiency are still not fully recognized. Here, we report on the enormous impact of the polymer thickness on the possibility of fabrication of extremely high-amplitude surface deformations. We performed prolonged holographic recordings on the layers of the same azobenzene poly(ether imide), which had substantially different optical transmittances at the recording wavelength and revealed that the depths of the inscribed relief structures increased with the polymer thickness from a nondetectable value up to almost 2 µm, unaffected by the presence of a polymer-glass substrate interface in either sample.
View Article and Find Full Text PDFDesign of soft x-ray high diffraction efficiency and spectral flux Au/Ni bilayer coated laminar-type diffraction grating for objective soft x-ray flat-field spectrograph in a region of 250-550 eV.
Rev Sci Instrum
December 2024
Institute of Multidisciplinary Research for Advanced Materials (IMRAM), Tohoku University, Aoba-ku, Sendai, Miyagi 980-8577, Japan.
An objective soft x-ray flat-field spectrograph employing a laminar-type bilayer coated, varied-line-spacing, spherical grating was designed to improve the detection limit and sensitivity of soft x-ray flat-field spectrographs in a region of 250-550 eV. As a design criterion, spectral flux, SF, [Hatano et al., Appl.
View Article and Find Full Text PDFA Novel Approach in Cancer Diagnosis: Integrating Holography Microscopic Medical Imaging and Deep Learning Techniques - Challenges and Future Trends.
Biomed Phys Eng Express
December 2024
CSE, Islamic University of Science and Technology, Awantipora, Awantipora, Jammu and Kashmir, 192122, INDIA.
Medical imaging is pivotal in early disease diagnosis, providing essential insights that enable timely and accurate detection of health anomalies. Traditional imaging techniques, such as Magnetic Resonance Imaging (MRI), Computer Tomography (CT), ultrasound, and Positron Emission Tomography (PET), offer vital insights into three-dimensional structures but frequently fall short of delivering a comprehensive and detailed anatomical analysis, capturing only amplitude details. Three-dimensional holography microscopic medical imaging provides a promising solution by capturing the amplitude (brightness) and phase (structural information) details of biological structures.
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