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G-Quadruplex-Filtered Selective Ion-to-Ion Current Amplification for Non-Invasive Ion Monitoring in Real Time.
Adv Mater
October 2023
Department of Materials Science & Engineering, Pohang University of Science and Technology (POSTECH), Pohang, Gyeongbuk, 37673, South Korea.
Living cells efflux intracellular ions for maintaining cellular life, so intravital measurements of specific ion signals are of significant importance for studying cellular functions and pharmacokinetics. In this work, de novo synthesis of artificial K -selective membrane and its integration with polyelectrolyte hydrogel-based open-junction ionic diode (OJID) is demonstrated, achieving a real-time K -selective ion-to-ion current amplification in complex bioenvironments. By mimicking biological K channels and nerve impulse transmitters, in-line K -binding G-quartets are introduced across freestanding lipid bilayers by G-specific hexylation of monolithic G-quadruplex, and the pre-filtered K flow is directly converted to amplified ionic currents by the OJID with a fast response time at 100 ms intervals.
View Article and Find Full Text PDFCorrelation between Infrared Absorption and Lithium Sublattice Disorder in Magnesium-Doped Lithium Niobate.
Materials (Basel)
January 2023
Centro de Ciências e Tecnologias Nucleares, Instituto Superior Técnico, Universidade de Lisboa, Estrada Nacional 10, km 139.7, P-2695-066 Bobadela, Portugal.
Lithium niobate is a ferro- and piezoelectric material with excellent optical properties and a wide variety of applications. The defect structures of congruent and Mg-doped crystals are still under intense discussion. In this work, undoped lithium niobate and magnesium-doped lithium niobate grown from congruent melt with the addition of 0 to 9 mol% MgO were investigated by infrared absorption, establishing the dependence of the absorbance on the Mg-doping level in two bands related to OH- stretching vibrations.
View Article and Find Full Text PDFNb Texture Evolution and Interdiffusion in Nb/Si-Layered Systems.
ACS Appl Mater Interfaces
July 2021
Industrial Focus Group XUV Optics, MESA+ Institute for Nanotechnology, University of Twente, Enschede 7500 AE Enschede, The Netherlands.
In this paper, we present a detailed study on the microstructure evolution and interdiffusion in Nb/Si-layered systems. Interlayer formation during the early stages of growth in sputter-deposited Nb-on-Si and Si-on-Nb bilayer systems is studied using a high-sensitivity low-energy ion-scattering technique. An asymmetric intermixing behavior is observed, where the Si-on-Nb interface is ∼2× thinner than the Nb-on-Si interface, and it is explained by the surface-energy difference between Nb and Si.
View Article and Find Full Text PDFConsequences of supplying methyl donors during pregnancy on the methylome of the offspring from lactating and non-lactating dairy cattle.
PLoS One
January 2018
Blanca, Hostalets de Tost, Spain.
The aim of this study was to evaluate the potential effects of methyl donor supplementation of pregnant animals in the presence or absence of a concomitant lactation on the methylome of the offspring. Twenty Holstein cows, 10 nulliparous (non-lactating while pregnant) and 10 multiparous (lactating while pregnant) were blocked by parity and randomly assigned to an i.m.
View Article and Find Full Text PDFStructural and functional roles of the N- and C-terminal extended modules in channelrhodopsin-1.
Photochem Photobiol Sci
September 2015
Division of Pharmaceutical Sciences, Okayama University, Okayama, 700-8530, Japan.
Channelrhodopsins have become a focus of interest because of their ability to control neural activity by light, used in a technology called optogenetics. The channelrhodopsin in the eukaryote Chlamydomonas reinhardtii (CrChR-1) is a light-gated cation channel responsible for motility changes upon photo-illumination and a member of the membrane-embedded retinal protein family. Recent crystal structure analysis revealed that CrChR-1 has unique extended modules both at its N- and C-termini compared to other microbial retinal proteins.
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