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MAO-B-triggered reaction for an optical triple-signal assay and AND logic gate application based on PEI-functionalized silver nanoparticles.
Mikrochim Acta
January 2025
School of Pharmacy, Lanzhou University, Lanzhou, 730000, P. R. China.
A novel analytical method was designed and developed that exhibited ultraviolet-visible (UV-Vis), fluorescence (FL), and resonance Rayleigh scattering (RRS) signals for straightforward and comprehensive determination of monoamine oxidase B (MAO-B) using polyethylenimine-functionalized silver nanoparticles (PEI-Ag NPs). Through a facile one-step experiment, and NaOH assisted, in an aqueous solution of 100 ℃ for 40 min PEI reacted with AgNO to generate PEI-Ag NPs with a yellow color and weak blue fluorescence. Interestingly, phenylacetaldehyde (PAA), a specific product of MAO-B, causes significant enhancement of the three optical signals of UV-Vis, FL, and RRS.
View Article and Find Full Text PDFMicrovascular blood flow ultrasound imaging with microbubble-based H-Scan technology.
Med Biol Eng Comput
January 2025
Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Science, Suzhou, 215613, China.
Ultrasound blood flow imaging plays a crucial role in the diagnosis of cardiovascular and cerebrovascular diseases. Conventional ultrafast ultrasound plane-wave imaging techniques have limited capabilities in microvascular imaging. To enhance the quality of blood flow imaging, this study proposes a microbubble-based H-Scan ultrasound imaging technique.
View Article and Find Full Text PDFCoomassie Brilliant Blue Induces Coiled-Coil Aggregation in Lysozyme at pH 7.4 by Hydrophobic and Electrostatic Forces.
ACS Omega
January 2025
Bioinformatics Programming Lab, Department of Biotechnology, School of Bio Sciences and Technology, VIT, Vellore 632014, India.
Several neurodegenerative diseases are associated with the deposition of amyloid fibrils. Although these diseases are irreversible, knowing the aggregation mechanism is useful in developing drugs that can arrest or decrease the aggregation rate. In this study, we are interested in investigating the effect of Coomassie brilliant blue (CBB G-250) on the aggregation of hen egg white lysozyme (HEWL) at pH 7.
View Article and Find Full Text PDFOn-treatment dynamics of circulating extracellular vesicles in the first-line setting of patients with advanced non-small cell lung cancer: the LEXOVE prospective study.
Mol Oncol
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Department of Precision Medicine in Medical, Surgical and Critical Care (Me.Pre.C.C.), University of Palermo, Italy.
Extracellular vesicle (EV) monitoring can complement clinical assessment of cancer response. In this study, patients with advanced non-small cell lung cancer (NSCLC) undergoing osimertinib, alectinib, pembrolizumab or platinum-based chemotherapy ± pembrolizumab were enrolled. EVs were characterized using Bradford assay to quantify the circulating cell-free EV protein content (cfEV), and dynamic light scattering to assess Rayleigh ratio excess at 90°, z-averaged hydrodynamic diameter and polydispersity index.
View Article and Find Full Text PDFSelf-Supervised and Zero-Shot Learning in Multi-Modal Raman Light Sheet Microscopy.
Sensors (Basel)
December 2024
CeMOS Research and Transfer Center, Mannheim University of Applied Sciences, 68163 Mannheim, Germany.
Advancements in Raman light sheet microscopy have provided a powerful, non-invasive, marker-free method for imaging complex 3D biological structures, such as cell cultures and spheroids. By combining 3D tomograms made by Rayleigh scattering, Raman scattering, and fluorescence detection, this modality captures complementary spatial and molecular data, critical for biomedical research, histology, and drug discovery. Despite its capabilities, Raman light sheet microscopy faces inherent limitations, including low signal intensity, high noise levels, and restricted spatial resolution, which impede the visualization of fine subcellular structures.
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