[The use of alternative sources of electrons in mono-oxygenase reactions catalyzed by cytochrome P450. Physico-chemical methods as factors acting on enzymatic activity of proteins].

Influence of physico-chemical methods on catalytic activities of enzymes, (cytochrome P450 in particular), has been reviewed. Authors discussed the properties of semisynthetic flavohemoproteins based on cytochrome P450. With emphasis on recovery of alternative electron sources for redox enzymes. As alternative electron sources we have discussed electrochemical methods which allow electrons to be used as a reagent for redox system and photoreduction of photosensitive molecules. The effects of the chemical modification, high pressure, temperature, organic solvents on enzyme activity have also been analysed.