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Multiphoton and Harmonic Imaging of Microarchitected Materials.
ACS Appl Mater Interfaces
January 2025
Laser Thermal Laboratory, Department of Mechanical Engineering, University of California, Berkeley, California 94720, United States.
Article Synopsis
- Microadditive manufacturing enables the creation of intricate nano- and microscale components, leading to advancements in various industries.
- This research explores two-photon and three-photon fluorescence imaging, along with third-harmonic generation microscopy, to analyze complex lattice structures produced by multiphoton lithography.
- The study reveals that multiphoton fluorescence imaging provides better depth penetration and nondestructive identification of internal modifications and defects, improving quality control in microadditively manufactured products.
Radiative corrections: from medium to high energy experiments.
Eur Phys J A Hadron Nucl
April 2024
Institute of Nuclear Physics, Johannes Gutenberg-Universität, 55099 Mainz, Germany.
Radiative corrections are crucial for modern high-precision physics experiments, and are an area of active research in the experimental and theoretical community. Here we provide an overview of the state of the field of radiative corrections with a focus on several topics: lepton-proton scattering, QED corrections in deep-inelastic scattering, and in radiative light-hadron decays. Particular emphasis is placed on the two-photon exchange, believed to be responsible for the proton form-factor discrepancy, and associated Monte-Carlo codes.
View Article and Find Full Text PDFRational Molecular Design for Balanced Locally Excited and Charge- Transfer Nature for Two-Photon Absorption Phenomenon and Highly Efficient TADF-Based OLEDs.
Angew Chem Int Ed Engl
November 2024
Center for Organic Photonics and Electronics Research (OPERA) and Department of Applied Chemistry, Kyushu University, 744 Motooka, Nishi, Fukuoka, 819-0395, Japan.
The pursuit of highly efficient thermally activated delayed fluorescence (TADF) emitters with two-photon absorption (2PA) character is hampered by the concurrent achievement of a small singlet-triplet energy gap (ΔE) and high photoluminescence quantum yield (Φ). Here, by introducing a terephthalonitrile unit into a sterically crowded donor-π-donor structure, inducing a hybrid electronic excitation character, we designed unique TADF emitters possessing 2PA ability. This rational molecular design was achieved through a main π-conjugated donor-acceptor-donor backbone in line with locally excited feature renders a large oscillator strength and transition dipole moment, maintaining a high 2PA cross-section value.
View Article and Find Full Text PDFSimultaneous assessment of NAD(P)H and flavins with multispectral fluorescence lifetime imaging microscopy at a single excitation wavelength of 750 nm.
J Biomed Opt
October 2024
Privolzhsky Research Medical University, Institute of Experimental Oncology and Biomedical Technologies, Novgorod, Russia.
Significance: Autofluorescence characteristics of the reduced nicotinamide adenine dinucleotide and oxidized flavin cofactors are important for the evaluation of the metabolic status of the cells. The approaches that involve a detailed analysis of both spectral and time characteristics of the autofluorescence signals may provide additional insights into the biochemical processes in the cells and biological tissues and facilitate the transition of spectral fluorescence lifetime imaging into clinical applications.
Aim: We present the experiments on multispectral fluorescence lifetime imaging with a detailed analysis of the fluorescence decays and spectral profiles of the reduced nicotinamide adenine dinucleotide and oxidized flavin under a single excitation wavelength aimed at understanding whether the use of multispectral detection is helpful for metabolic imaging of cancer cells.
A fast and responsive voltage indicator with enhanced sensitivity for unitary synaptic events.
Neuron
November 2024
Department of Bioengineering, Stanford University, Stanford, CA 94305, USA; Department of Neurobiology, Stanford University, Stanford, CA 94305, USA. Electronic address:
Article Synopsis
- Researchers developed a new genetically encoded voltage indicator (GEVI) called ASAP5, which offers improved detection of excitatory postsynaptic potentials (EPSPs) and action potentials (APs) with better signal quality compared to previous indicators.
- ASAP5 was able to detect both spiking and subthreshold neuronal activities in real-time, showing its effectiveness in both animal models and cultured human neurons, even capturing small EPSPs of about 1-mV.
- The study revealed that EPSP amplitudes decrease as they move away from the source, with further implications for using voltage imaging in studying neuronal dysfunction related to diseases, including those affecting human neurons.
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